Combating Pathogenic Immune Evasion: Sialidase-Activated Thermally Delayed Fluorescence for Probing and Modulating Host–Pathogen Interactions

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Innate immunity represents the primary defense against invasive pathogens with phagocytosis playing a central role in host and mediating immune inflammatory responses. However, such as Clostridium perfringens have developed strategies to overcome phagocytic clearance. Developing molecular tools identify target key factors pathogenic evasion can deepen our understanding of host–pathogen interactions aid exploring novel therapeutic strategies. As enzyme sialylation process C. perfringens, virulence factor sialidase is potential for investigating evasion. Herein, "turn-on" thermally activated delayed fluorescent probe SA-HBT-PXZ highly selective sensitive sensor, enabling time-resolved fluorescence imaging live bacterial cells, tissue sections, even infected mice. Furthermore, successfully employed screen inhibitors based on prompt emissions. The identified lead compounds effectively inhibit activity sialidases from leading an increased level differentiation macrophages into M1 subtype. This, turn, enhances ultimately suppresses escape bacteria. Our study provides infections.

Язык: Английский

Universal Immune Learning Ability in Immune and Non-Immune Cells (A Review)

Russian Journal of Bioorganic Chemistry, Год журнала: 2024, Номер 50(6), С. 2209 - 2218

Опубликована: Дек. 1, 2024

Язык: Английский