Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy

Neuron, Год журнала: 2020, Номер 106(3), С. 369 - 387

Опубликована: Май 1, 2020

Язык: Английский

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Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Июль 4, 2019

Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural molecular information from intact tissues. However, current microscopes imposed constraints on the size, shape, number of specimens, compatibility with various protocols. Here we present a multi-immersion open-top microscope that enables simple mounting multiple specimens processed variety protocols, which will facilitate wide adoption by preclinical researchers clinical laboratories. In particular, geometry provides unsurpassed versatility interface range accessory technologies future.

Язык: Английский

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Light-sheet microscopy of cleared tissues with isotropic, subcellular resolution

Nature Methods, Год журнала: 2019, Номер 16(11), С. 1109 - 1113

Опубликована: Окт. 31, 2019

Язык: Английский

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A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics

eLife, Год журнала: 2020, Номер 9

Опубликована: Ноя. 12, 2020

We present an oblique plane microscope (OPM) that uses a bespoke glass-tipped tertiary objective to improve the resolution, field of view, and usability over previous variants. Owing its high numerical aperture optics, this achieves lateral axial resolutions are comparable square illumination mode lattice light-sheet microscopy, but in user friendly versatile format. Given performance, we demonstrate high-resolution imaging clathrin-mediated endocytosis, vimentin, endoplasmic reticulum, membrane dynamics, Natural Killer-mediated cytotoxicity. Furthermore, image biological phenomena would be otherwise challenging or impossible perform traditional geometry, including cell migration through confined spaces within microfluidic device, subcellular photoactivation Rac1, diffusion cytoplasmic rheological tracers at volumetric rate 14 Hz, large view neurons, developing embryos, centimeter-scale tissue sections.

Язык: Английский

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Rapid image deconvolution and multiview fusion for optical microscopy

Nature Biotechnology, Год журнала: 2020, Номер 38(11), С. 1337 - 1346

Опубликована: Июнь 29, 2020

Язык: Английский

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Enhanced Data Security of Communication System Using Combined Encryption and Steganography

International Journal of Interactive Mobile Technologies (iJIM), Год журнала: 2021, Номер 15(16), С. 144 - 144

Опубликована: Авг. 23, 2021

<h2>Data security has become a paramount necessity and more obligation in daily life. Most of our systems can be hacked, it causes very high risks to confidential files inside the systems. Therefore, for various reasons, we use methods save as much possible on this data, regardless its different forms, texts, pictures, videos, etc. In paper, mainly rely storing basic image which should protected another after changing formal composites using DWT wavelet transform. The process zeroing sites their contents technique is used carry components main image. Then them mathematically by exponential function. result obtain fully encrypted required from detection discrimination hidden behind proposed system contains two algorithms. first algorithm encryption hiding, but second designed returning decoding original state with efficiently.</h2>

Язык: Английский

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A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues

Nature Methods, Год журнала: 2022, Номер 19(5), С. 613 - 619

Опубликована: Май 1, 2022

Язык: Английский

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Harnessing non-destructive 3D pathology

Nature Biomedical Engineering, Год журнала: 2021, Номер 5(3), С. 203 - 218

Опубликована: Фев. 15, 2021

Язык: Английский

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Tissue clearing

Nature Reviews Methods Primers, Год журнала: 2021, Номер 1(1)

Опубликована: Дек. 16, 2021

Язык: Английский

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Stitching and registering highly multiplexed whole-slide images of tissues and tumors using ASHLAR

Bioinformatics, Год журнала: 2022, Номер 38(19), С. 4613 - 4621

Опубликована: Авг. 16, 2022

Abstract Motivation Stitching microscope images into a mosaic is an essential step in the analysis and visualization of large biological specimens, particularly human animal tissues. Recent approaches to highly multiplexed imaging generate high-plex data from sequential rounds lower-plex imaging. These methods promise yield precise molecular single-cell information on cellular neighborhoods tissue architecture. However, attaining with accuracy requires robust image stitching registration capabilities that are not met by existing methods. Results We describe development testing ASHLAR, Python tool for coordinated 103 or more individual accurate whole-slide mosaics. ASHLAR reads formats most commercial microscopes slide scanners, we show it performs better than open-source software. outputs standard OME-TIFF ready other tools recently developed pipelines. Availability implementation written available under MIT license at https://github.com/labsyspharm/ashlar. The newly published underlying this article Sage Synapse https://dx.doi.org/10.7303/syn25826362; availability previously re-analyzed described Supplementary Table S4. An informational website user guides test https://labsyspharm.github.io/ashlar/. Bioinformatics online.

Язык: Английский

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