Functional ultrasound imaging reveals microvascular rarefaction, decreased cerebral blood flow, and impaired neurovascular coupling in a mouse model of paclitaxel-induced chemobrain DOI Creative Commons

Siva Sai Chandragiri,

Ádám Nyúl‐Tóth, Sharon Negri

и другие.

GeroScience, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Chemotherapy-induced cognitive impairment (CICI), often referred to as "chemobrain," significantly affects the quality of life in cancer survivors. Although traditionally attributed neuronal toxicity, emerging evidence suggests a key role cerebrovascular dysfunction its pathogenesis. We hypothesized that paclitaxel (PTX, Taxol) treatment induces long-term dysfunction, including microvascular rarefaction, impaired neurovascular coupling (NVC), and altered cerebral blood flow (CBF), which contribute CICI. Using clinically relevant PTX regimen non-tumor-bearing mice, we evaluated effects on health. Ultrasound localization microscopy (ULM) functional ultrasound imaging (fUS) were employed assess density, CBF, NVC. resulted significant reduction density cortex hippocampus, regions involved function. reduced velocity middle artery. Moreover, NVC responses, evidenced by diminished CBF increase response whisker stimulation, indicative reactive hyperemia. In conclusion, these findings demonstrate long-lasting NVC, dynamics, likely This study underscores critical health function highlights potential targeting pathways therapeutic approach for mitigating chemotherapy-induced deficits.

Язык: Английский

Functional ultrasound localization microscopy reveals brain-wide neurovascular activity on a microscopic scale DOI Creative Commons

Noémi Renaudin,

Charlie Demené, Alexandre Dizeux

и другие.

Nature Methods, Год журнала: 2022, Номер 19(8), С. 1004 - 1012

Опубликована: Авг. 1, 2022

The advent of neuroimaging has increased our understanding brain function. While most brain-wide functional imaging modalities exploit neurovascular coupling to map activity at millimeter resolutions, the recording responses microscopic scale in mammals remains privilege invasive electrophysiological or optical approaches, but is mostly restricted either cortical surface vicinity implanted sensors. Ultrasound localization microscopy (ULM) achieved transcranial cerebrovascular flow, up micrometre scales, by localizing intravenously injected microbubbles; however, long acquisition time required detect microbubbles within vessels so far ULM application mainly microvasculature structural imaging. Here we show how can be modified quantify hyperemia dynamically during activation reaching a 6.5-µm spatial and 1-s temporal resolution deep regions rat brain.

Язык: Английский

Процитировано

104
Super-resolution ultrasound microvascular imaging: Is it ready for clinical use? DOI Creative Commons
Pengfei Song, Jonathan M. Rubin, Matthew R. Lowerison

и другие.

Zeitschrift für Medizinische Physik, Год журнала: 2023, Номер 33(3), С. 309 - 323

Опубликована: Май 20, 2023

The field of super-resolution ultrasound microvascular imaging has been rapidly growing over the past decade. By leveraging contrast microbubbles as point targets for localization and tracking, pinpoints location microvessels measures their blood flow velocity. Super-resolution is first in vivo modality that can image micron-scale vessels at a clinically relevant depth without tissue destruction. These unique capabilities provide structural (vessel morphology) functional flow) assessments microvasculature on global local scale, which opens new doors many enticing preclinical clinical applications benefit from biomarkers. goal this short review to an update recent advancements imaging, with focus summarizing existing discussing prospects translating practice research. In review, we also brief introductions how works, does it compare other modalities, what are tradeoffs limitations audience who not familiar technology.

Язык: Английский

Процитировано

48
Transcranial volumetric imaging using a conformal ultrasound patch DOI
Sai Zhou,

Xiaoxiang Gao,

Geonho Park

и другие.

Nature, Год журнала: 2024, Номер 629(8013), С. 810 - 818

Опубликована: Май 22, 2024

Язык: Английский

Процитировано

27
Leptomeningeal collaterals regulate reperfusion in ischemic stroke and rescue the brain from futile recanalization DOI Creative Commons
Nadine Felizitas Binder, Mohamad El Amki, Chaim Glück

и другие.

Neuron, Год журнала: 2024, Номер 112(9), С. 1456 - 1472.e6

Опубликована: Фев. 26, 2024

Recanalization is the mainstay of ischemic stroke treatment. However, even with timely clot removal, many patients recover poorly. Leptomeningeal collaterals (LMCs) are pial anastomotic vessels yet-unknown functions. We applied laser speckle imaging, ultrafast ultrasound, and two-photon microscopy in a thrombin-based mouse model fibrinolytic treatment to show that LMCs maintain cerebral autoregulation allow for gradual reperfusion, resulting small infarcts. In mice poor LMCs, distal arterial segments collapse, deleterious hyperemia causes hemorrhage mortality after recanalization. silico analyses confirm relevance preserving perfusion region. Accordingly, undergoing thrombectomy, rapid reperfusion resulted hemorrhagic transformation unfavorable recovery. Thus, we identify as key components regulating preventing futile recanalization stroke. Future therapeutic interventions should aim enhance collateral function, allowing beneficial

Язык: Английский

Процитировано

25
Transthoracic ultrasound localization microscopy of myocardial vasculature in patients DOI Creative Commons
Jipeng Yan, B. K. Huang, Johanna Tonko

и другие.

Nature Biomedical Engineering, Год журнала: 2024, Номер 8(6), С. 689 - 700

Опубликована: Май 6, 2024

Myocardial microvasculature and haemodynamics are indicative of potential microvascular diseases for patients with symptoms coronary heart disease in the absence obstructive arteries. However, imaging structure flow within myocardium is challenging owing to small size vessels constant movement patient's heart. Here we show feasibility transthoracic ultrasound localization microscopy myocardial explanted pig hearts vivo. Through a customized data-acquisition processing pipeline cardiac phased-array probe, leveraged motion correction tracking reconstruct dynamics microcirculation. For four patients, two whom had impaired function, obtained super-resolution images vascular using data acquired breath hold. may facilitate understanding microcirculation management diseases.

Язык: Английский

Процитировано

21
Context-aware deep learning enables high-efficacy localization of high concentration microbubbles for super-resolution ultrasound localization microscopy DOI Creative Commons
YiRang Shin, Matthew R. Lowerison, Yike Wang

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 4, 2024

Abstract Ultrasound localization microscopy (ULM) enables deep tissue microvascular imaging by localizing and tracking intravenously injected microbubbles circulating in the bloodstream. However, conventional techniques require spatially isolated microbubbles, resulting prolonged time to obtain detailed maps. Here, we introduce LOcalization with Context Awareness (LOCA)-ULM, a learning-based microbubble simulation pipeline designed enhance performance high concentrations. In silico, LOCA-ULM enhanced detection accuracy 97.8% reduced missing rate 23.8%, outperforming methods up 17.4% 37.6% reduction. vivo rat brain imaging, revealed dense cerebrovascular networks adjacent microvessels undetected ULM. We further demonstrate superior of functional ULM (fULM) where significantly increased sensitivity fULM hemodynamic responses invoked whisker stimulations brain.

Язык: Английский

Процитировано

19
Probing the pressure dependence of sound speed and attenuation in bubbly media: Experimental observations, a theoretical model and numerical calculations DOI Creative Commons

Amin Jafari Sojahrood,

Q. Li,

Hossein Haghi

и другие.

Ultrasonics Sonochemistry, Год журнала: 2023, Номер 95, С. 106319 - 106319

Опубликована: Фев. 6, 2023

The problem of attenuation and sound speed bubbly media has remained partially unsolved. Comprehensive data regarding pressure-dependent changes the a medium are not available. Our theoretical understanding is limited to linear or semi-linear models, which accurate in regime large amplitude bubble oscillations. Here, by controlling size lipid coated bubbles (mean diameter ≈5.4μm), we report first time observation characterization simultaneous pressure dependence water below, at above microbubbles resonance (frequency range between 1-3 MHz). With increasing acoustic (between 12.5-100 kPa), frequency peak decreases while maximum minimum amplitudes increase. We propose nonlinear model for estimation dependent with good agreement experiments. calculations validated comparing models predictions. One major challenges previously developed significant overestimation higher void fractions (e.g. 0.005). addressed this incorporating bubble-bubble interactions results Influence increases pressure. Within examined exposure parameters, numerically show that, even low 5.1×10

Язык: Английский

Процитировано

41
Super-resolution ultrasound reveals cerebrovascular impairment in a mouse model of Alzheimer's Disease DOI Creative Commons
Matthew R. Lowerison, Nathiya Vaithiyalingam Chandra Sekaran, Zhijie Dong

и другие.

Journal of Neuroscience, Год журнала: 2024, Номер unknown, С. e1251232024 - e1251232024

Опубликована: Янв. 22, 2024

Increasing evidence has suggested a link between cerebrovascular disease and the cognitive impairment associated with Alzheimer's Disease. However, detailed descriptions of microvascular changes across brain regions how they relate to other more traditional pathology have been lacking. Additionally, efforts elucidate interplay cerebral function Disease progression are complicated by necessity probing deep-brain structures since early-stage typically involves hippocampal pathology. The purpose this study was examine in dynamics mouse model using cohorts that were age-matched wild-type controls. Data from both sexes included study. Super-resolution ultrasound localization microscopy revealed functional structural features throughout whole depth visualize quantify. We found decreases entorhinal flow velocity preceded derangements regional vascular density. Co-registered histological sectioning confirmed regionalized perfusion deficits seen on imaging, which co-localized amyloid beta plaque deposition. In addition providing global quantifications deep high local resolution, technology also permitted velocity-profile analysis individual vessels and, some cases, allowed for decoupling arterial venous contributions. These data suggest is an early pervasive feature may represent novel therapeutic target disease. Significance statement Studies impact difficulty imaging fidelity. demonstrate microscopy, super-resolution acoustic technique, capable cerebrovasculature entire at scale. This applied 5xFAD Disease, where it animals significant impairments cortex hippocampus compared age matched controls 3-month timepoint. Structural only observed 6-month-old timepoint, maintained function. findings occurs cascade.

Язык: Английский

Процитировано

12
ULTRA-SR Challenge: Assessment of Ultrasound Localization and TRacking Algorithms for Super-Resolution Imaging DOI
Marcelo Lerendegui, Kai Riemer, Γεώργιος Παπαγεωργίου

и другие.

IEEE Transactions on Medical Imaging, Год журнала: 2024, Номер 43(8), С. 2970 - 2987

Опубликована: Апрель 12, 2024

With the widespread interest and uptake of super-resolution ultrasound (SRUS) through localization tracking microbubbles, also known as microscopy (ULM), many algorithms have been developed. ULM can image centimeters into tissue in-vivo track microvascular flow non-invasively with sub-diffraction resolution. In a significant community effort, we organized challenge, Ultrasound Localization TRacking Algorithms for Super-Resolution (ULTRA-SR). The aims this paper are threefold: to describe challenge organization, data generation, winning algorithms; present metrics methods evaluating entrants; report results findings evaluation. Realistic datasets containing different clinical frequencies were simulated, using vascular physics, acoustic field simulation nonlinear bubble dynamics simulation. Based on these datasets, 38 submissions from 24 research groups evaluated against ground truth an evaluation framework six metrics, three tracking. In-vivo mouse brain human lymph node provided, performance assessed by expert panel. Winning described discussed. publicly available defined both valuable resource researchers benchmark software, identify optimized methods/software their data, provide insight current limits field. conclusion, Ultra-SR has provided benchmarking tools well direct comparison insights number state-of-the art algorithms.

Язык: Английский

Процитировано

11
Functional ultrasound imaging of human brain activity through an acoustically transparent cranial window DOI
Claire Rabut, Sumner L. Norman, Whitney S. Griggs

и другие.

Science Translational Medicine, Год журнала: 2024, Номер 16(749)

Опубликована: Май 29, 2024

Visualization of human brain activity is crucial for understanding normal and aberrant function. Currently available neural recording methods are highly invasive, have low sensitivity, cannot be conducted outside an operating room. Functional ultrasound imaging (fUSI) emerging technique that offers sensitive, large-scale, high-resolution imaging; however, fUSI performed through the adult skull. Here, we used a polymeric skull replacement material to create acoustic window compatible with monitor in single individual. Using vitro cerebrovascular phantom mimic vasculature vivo rodent cranial defect model, first, evaluated signal intensity signal-to-noise ratio polymethyl methacrylate (PMMA) implants different thicknesses or titanium mesh implant. We found rat could recorded high sensitivity PMMA implant using dedicated pulse sequence. then designed custom ultrasound-transparent patient undergoing reconstructive surgery after traumatic injury. showed record awake In video game “connect dots” task, demonstrated mapping decoding task-modulated cortical this guitar-strumming mapped additional task-specific responses. Our proof-of-principle study shows can as (200 μm) functional modality measuring acoustically transparent window.

Язык: Английский

Процитировано

11