Intraoperative
histology
is
essential
for
surgical
guidance
and
decision-making.
However,
frozen-sectioned
hematoxylin
eosin
(H&E)
staining
suffers
from
degraded
accuracy,
whereas
the
gold-standard
formalin-fixed
paraffin-embedded
(FFPE)
H&E
too
lengthy
intraoperative
use.
Stimulated
Raman
scattering
(SRS)
microscopy
has
shown
rapid
of
brain
tissue
with
lipid/protein
contrast
but
challenging
to
yield
images
identical
nucleic
acid-/protein-based
FFPE
stains
interpretable
pathologists.
Here,
we
report
development
a
semi-supervised
stimulated
CycleGAN
model
convert
fresh-tissue
SRS
using
unpaired
training
data.
Within
3
minutes,
virtual
(SRVH)
results
that
matched
perfectly
true
could
be
generated.
A
blind
validation
indicated
board-certified
neuropathologists
are
able
differentiate
histologic
subtypes
human
glioma
on
SRVH
hardly
conventional
images.
may
provide
diagnosis
superior
frozen
in
both
speed
extendable
other
types
solid
tumors.
Communications Biology,
Год журнала:
2023,
Номер
6(1)
Опубликована: Май 25, 2023
Abstract
Optical
microscopes
today
have
pushed
the
limits
of
speed,
quality,
and
observable
space
in
biological
specimens
revolutionizing
how
we
view
life
today.
Further,
specific
labeling
samples
for
imaging
has
provided
insight
into
functions.
This
enabled
label-based
microscopy
to
percolate
integrate
mainstream
science
research.
However,
use
labelfree
been
mostly
limited,
resulting
testing
bio-application
but
not
bio-integration.
To
enable
bio-integration,
such
need
be
evaluated
their
timeliness
answer
questions
uniquely
establish
a
long-term
growth
prospect.
The
article
presents
key
label-free
optical
discusses
integrative
potential
research
unperturbed
analysis
samples.
Advanced Optical Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 30, 2025
Abstract
Raman
scattering
is
characterized
by
the
inherently
weak
inelastic
of
photons,
influenced
molecular
vibrations
or
rotations.
Recent
advances
have
shifted
from
traditional
electromagnetic
enhancement
methods
to
chemically
enhanced
scattering,
offering
significant
advantages.
However,
these
advancements
typically
depended
on
indirect
and
empirical
models.
This
article
introduces
a
systematic
method
for
rational
design
engineering
chemical
scattering.
involves
identifying
promising
enhancers
optimizing
their
morphology
composition
elucidating
photochemical
properties
mapping
charge‐transfer
pathways
with
target
molecules
using
transient
absorption
spectroscopy
(TAS),
cyclic
voltammetry
(CV),
density
functional
theory
(DFT)
calculations.
Employing
this
method,
work
has
developed
series
rationally
designed
made
conducting
polymers
(CPs),
such
as
poly(3,4‐ethylenedioxythiophene)
(PEDOT),
optimized
morphological
traits
compositions.
These
significantly
improve
surface‐enhanced
(SERS),
achieving
reproducible
factor
up
10
6
,
boost
lasing,
remarkable
40‐fold
increase
in
energy
conversion
efficiency.
Nanomaterials,
Год журнала:
2025,
Номер
15(7), С. 563 - 563
Опубликована: Апрель 7, 2025
Stimulated
Raman
Scattering
(SRS)
nanoscopy
imaging
offers
unprecedented
insights
into
tissue
molecular
architecture
but
often
requires
stitching
multiple
high-resolution
tiles
to
capture
large
fields
of
view.
This
process
is
time-consuming
and
frequently
introduces
vignetting
artifacts—grid-like
intensity
fluctuations
that
degrade
image
quality
hinder
downstream
quantitative
analyses
processing
such
as
super-resolution
deconvolution.
We
present
VIgnetted
Stitched-Tile
Adjustment
using
Morphological
Adaptive
Processing
(VISTAmap),
a
simple
tool
corrects
these
shading
artifacts
directly
on
the
final
stitched
image.
VISTAmap
automatically
detects
tile
grid
configuration
by
analyzing
frequency
variations
then
applies
sequential
morphological
operations
homogenize
In
contrast
conventional
approaches
require
increased
overlap
or
pre-acquisition
background
sampling,
pragmatic,
post-processing
solution
without
need
for
separate
individual
images.
work
addresses
pressing
concerns
delivering
robust,
efficient
strategy
enhancing
mosaic
uniformity
in
modern
nanoscopy,
where
smallest
details
make
tremendous
impacts.
Light Science & Applications,
Год журнала:
2023,
Номер
12(1)
Опубликована: Июнь 5, 2023
Far-field
chemical
microscopy
providing
molecular
electronic
or
vibrational
fingerprint
information
opens
a
new
window
for
the
study
of
three-dimensional
biological,
material,
and
systems.
Chemical
provides
nondestructive
way
identification
without
exterior
labels.
However,
diffraction
limit
optics
hindered
it
from
discovering
more
details
under
resolution
limit.
Recent
development
super-resolution
techniques
gives
enlightenment
to
open
this
door
behind
far-field
microscopy.
Here,
we
review
recent
advances
that
have
pushed
boundary
in
terms
spatial
resolution.
We
further
highlight
applications
biomedical
research,
material
characterization,
environmental
study,
cultural
heritage
conservation,
integrated
chip
inspection.
GEN Biotechnology,
Год журнала:
2023,
Номер
2(3), С. 247 - 261
Опубликована: Июнь 1, 2023
Studies
have
shown
that
brain
lipid
metabolism
is
associated
with
biological
aging
and
influenced
by
dietary
genetic
manipulations;
however,
the
underlying
mechanisms
are
elusive.
High-resolution
imaging
techniques
propose
a
novel
potent
approach
to
understanding
metabolic
dynamics
in
situ.
Applying
deuterium
water
(D2O)
probing
stimulated
Raman
scattering
(DO-SRS)
microscopy,
we
revealed
activity
Drosophila
decreased
sex-dependent
manner.
Female
flies
showed
an
earlier
occurrence
of
turnover
decrease
than
males.
Dietary
restriction
(DR)
downregulation
insulin/IGF-1
signaling
(IIS)
pathway,
two
scenarios
for
lifespan
extension,
led
significant
enhancements
old
flies.
Combining
SRS
deuterated
bioorthogonal
probes
(deuterated
glucose
acetate),
discovered
that,
under
DR
treatment
IIS
shifted
use
acetate
as
major
carbon
source
synthesis.
For
first
time,
our
study
directly
visualizes
quantifies
spatiotemporal
alterations
at
single
organelle
(lipid
droplet)
level.
Our
not
only
demonstrates
new
studying
situ
but
also
illuminates
interconnection
aging,
dietary,
manipulations
on
regulation.
