Опубликована: Апрель 14, 2025

Abstract Biomolecular networks can dynamically encode information, generating time-varying patterns of activity in response to an input. Here we find that dynamic encoding also be performed by individual proteins. BcLOV4 is optogenetic protein uniquely displays pulsatory activation a step input light, and dynamics shaped both light temperature. However, how the generates this step-to-pulse not understood. combined live cell imaging simulations pulse results from intramolecular incoherent feedforward loop (IFFL) implemented competitive interactions between domains separately respond or We identified these light- temperature-sensitive regions found they implement IFFL competitively caging region. Structural sequence analysis revealed temperature-responsive which allowed experimental tuning suggested has occurred throughout evolution. These findings enabled generation more thermostable tools modular thermosensitive domain endowed thermogenetic control over unrelated Our uncover principles combinatorial signal processing proteins will fuel development sophisticated compact synthetic systems. One-Sentence Summary Individual information through their component domains, revealing for complex natural engineered

Язык: Английский