Modular-designed engineered bacteria for precision tumor immunotherapy via spatiotemporal manipulation by magnetic field

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Март 23, 2023

Abstract Micro-nano biorobots based on bacteria have demonstrated great potential for tumor diagnosis and treatment. The bacterial gene expression drug release should be spatiotemporally controlled to avoid in healthy tissues undesired toxicity. Herein, we describe an alternating magnetic field-manipulated tumor-homing developed by genetically modifying engineered Escherichia coli with Fe 3 O 4 @lipid nanocomposites. After accumulating orthotopic colon tumors female mice, the paramagnetic nanoparticles enable receive convert signals into heat, thereby initiating of lysis proteins under control a heat-sensitive promoter. then lyse, releasing its anti-CD47 nanobody cargo, that is pre-expressed within bacteria. robust immunogenicity lysate cooperates activate both innate adaptive immune responses, generating antitumor effects against not only but also distal mice. magnetically constant field-controlled motion enhanced targeting increased therapeutic efficacy. Thus, behavior can manipulated vivo field, achieving tumor-specific CD47 blockage precision immunotherapy.

Язык: Английский

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Kalium channelrhodopsins are natural light-gated potassium channels that mediate optogenetic inhibition

Nature Neuroscience, Год журнала: 2022, Номер 25(7), С. 967 - 974

Опубликована: Июнь 20, 2022

Язык: Английский

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Two-photon calcium imaging of neuronal activity

Nature Reviews Methods Primers, Год журнала: 2022, Номер 2(1)

Опубликована: Сен. 1, 2022

Язык: Английский

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Recent Advances on High‐Speed and Holographic Two‐Photon Direct Laser Writing

Advanced Functional Materials, Год журнала: 2023, Номер 33(39)

Опубликована: Фев. 22, 2023

Abstract Two‐Photon Lithography, thanks to its very high sub‐diffraction resolution, has become the lithographic technique par excellence in applications requiring small feature sizes and complex 3D pattering. Despite this, fabrication times required for extended structures remain much longer than those of other competing techniques (UV mask lithography, nanoimprinting, etc.). Its low throughput prevents wide adoption industrial applications. To increase it, over years different solutions have been proposed, although their usage is difficult generalize may be limited depending on specific application. A promising strategy further opening a concrete window industry, lies combination with holography approaches: this way it possible generate dozens foci from single laser beam, thus parallelizing periodic structures, or engineer intensity distribution writing plane way, obtaining microstructures exposure. Here, fundamental concepts behind high‐speed Lithography are discussed, literary production recent that exploits such reviewed, contextualized according topic covered.

Язык: Английский

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Resolving the prefrontal mechanisms of adaptive cognitive behaviors: A cross-species perspective

Neuron, Год журнала: 2023, Номер 111(7), С. 1020 - 1036

Опубликована: Апрель 1, 2023

Язык: Английский

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The Roles of Optogenetics and Technology in Neurobiology: A Review

Frontiers in Aging Neuroscience, Год журнала: 2022, Номер 14

Опубликована: Апрель 19, 2022

Optogenetic is a technique that combines optics and genetics to control specific neurons. This usually uses adenoviruses encode photosensitive protein. The adenovirus may concentrate in neural region. By shining light on the target nerve region, protein encoded by controlled. Photosensitive proteins controlled can selectively allow ions inside outside cell membrane pass through, resulting inhibition or activation effects. Due high precision minimally invasive, optogenetics has achieved good results many fields, especially field of neuron functions circuits. Significant advances have also been made study clinical diseases. review focuses research neurobiology. These include how use cells, circuits, treat diseases changing state We hoped this will give comprehensive understanding progress

Язык: Английский

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Prediction-error neurons in circuits with multiple neuron types: Formation, refinement, and functional implications

Proceedings of the National Academy of Sciences, Год журнала: 2022, Номер 119(13)

Опубликована: Март 23, 2022

Significance An influential idea in neuroscience is that neural circuits do not only passively process sensory information but rather actively compare them with predictions thereof. A core element of this comparison prediction-error neurons, the activity which changes upon mismatches between actual and predicted stimuli. While it has been shown these neurons come different variants, largely unresolved how they are simultaneously formed shaped by highly interconnected networks. By using a computational model, we study circuit-level mechanisms give rise to variants neurons. Our results shed light on formation, refinement, robustness circuits, an important step toward better understanding predictive processing.

Язык: Английский

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Holographic stimulation of opposing amygdala ensembles bidirectionally modulates valence-specific behavior via mutual inhibition

Neuron, Год журнала: 2023, Номер 112(4), С. 593 - 610.e5

Опубликована: Дек. 11, 2023

Язык: Английский

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Whole-brain mapping of effective connectivity by fMRI with cortex-wide patterned optogenetics

Neuron, Год журнала: 2023, Номер 111(11), С. 1732 - 1747.e6

Опубликована: Март 30, 2023

Язык: Английский

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More than double the fun with two-photon excitation microscopy

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Март 26, 2024

Abstract For generations researchers have been observing the dynamic processes of life through lens a microscope. This has offered tremendous insights into biological phenomena that span multiple orders time- and length-scales ranging from pure magic molecular reorganization at membrane immune cells, to cell migration differentiation during development or wound healing. Standard fluorescence microscopy techniques offer glimpses such in vitro, however, when applied intact systems, they are challenged by reduced signal strengths signal-to-noise ratios result deeper imaging. As remedy, two-photon excitation (TPE) takes special place, because it allows us investigate vivo, their natural environment, even living animal. Here, we review fundamental principles underlying TPE aimed basic advanced users interested adopting for intravital We focus on applications neurobiology, present current trends towards faster, wider imaging, discuss combination with photon counting technologies metabolic imaging spectroscopy, as well highlight outstanding issues drawbacks application these methodologies.

Язык: Английский

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