Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(51)
Опубликована: Окт. 12, 2023
Abstract
To
interrogate
neural
circuits
and
crack
their
codes,
in
vivo
brain
activity
imaging
must
be
combined
with
spatiotemporally
precise
stimulation
three
dimensions
using
genetic
or
pharmacological
specificity.
This
challenge
requires
deep
penetration
focusing
as
provided
by
infrared
light
multiphoton
excitation,
has
promoted
two‐photon
photopharmacology
optogenetics.
However,
three‐photon
remains
to
demonstrated.
We
report
the
regulation
of
neuronal
zebrafish
larvae
excitation
a
photoswitchable
muscarinic
agonist
at
50
pM,
billion‐fold
lower
concentration
than
used
for
uncaging,
mid‐infrared
1560
nm,
longest
reported
photoswitch
wavelength.
Robust,
physiologically
relevant
photoresponses
allow
modulating
wild‐type
animals
spatiotemporal
precision.
Computational
calculations
predict
that
azobenzene‐based
ligands
have
high
absorption
cross‐section
can
directly
pulsed
light.
The
expansion
pharmacology
will
deeply
impact
basic
neurobiology
neuromodulation
phototherapies.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Март 23, 2023
Abstract
Micro-nano
biorobots
based
on
bacteria
have
demonstrated
great
potential
for
tumor
diagnosis
and
treatment.
The
bacterial
gene
expression
drug
release
should
be
spatiotemporally
controlled
to
avoid
in
healthy
tissues
undesired
toxicity.
Herein,
we
describe
an
alternating
magnetic
field-manipulated
tumor-homing
developed
by
genetically
modifying
engineered
Escherichia
coli
with
Fe
3
O
4
@lipid
nanocomposites.
After
accumulating
orthotopic
colon
tumors
female
mice,
the
paramagnetic
nanoparticles
enable
receive
convert
signals
into
heat,
thereby
initiating
of
lysis
proteins
under
control
a
heat-sensitive
promoter.
then
lyse,
releasing
its
anti-CD47
nanobody
cargo,
that
is
pre-expressed
within
bacteria.
robust
immunogenicity
lysate
cooperates
activate
both
innate
adaptive
immune
responses,
generating
antitumor
effects
against
not
only
but
also
distal
mice.
magnetically
constant
field-controlled
motion
enhanced
targeting
increased
therapeutic
efficacy.
Thus,
behavior
can
manipulated
vivo
field,
achieving
tumor-specific
CD47
blockage
precision
immunotherapy.
Advanced Functional Materials,
Год журнала:
2023,
Номер
33(39)
Опубликована: Фев. 22, 2023
Abstract
Two‐Photon
Lithography,
thanks
to
its
very
high
sub‐diffraction
resolution,
has
become
the
lithographic
technique
par
excellence
in
applications
requiring
small
feature
sizes
and
complex
3D
pattering.
Despite
this,
fabrication
times
required
for
extended
structures
remain
much
longer
than
those
of
other
competing
techniques
(UV
mask
lithography,
nanoimprinting,
etc.).
Its
low
throughput
prevents
wide
adoption
industrial
applications.
To
increase
it,
over
years
different
solutions
have
been
proposed,
although
their
usage
is
difficult
generalize
may
be
limited
depending
on
specific
application.
A
promising
strategy
further
opening
a
concrete
window
industry,
lies
combination
with
holography
approaches:
this
way
it
possible
generate
dozens
foci
from
single
laser
beam,
thus
parallelizing
periodic
structures,
or
engineer
intensity
distribution
writing
plane
way,
obtaining
microstructures
exposure.
Here,
fundamental
concepts
behind
high‐speed
Lithography
are
discussed,
literary
production
recent
that
exploits
such
reviewed,
contextualized
according
topic
covered.
Frontiers in Aging Neuroscience,
Год журнала:
2022,
Номер
14
Опубликована: Апрель 19, 2022
Optogenetic
is
a
technique
that
combines
optics
and
genetics
to
control
specific
neurons.
This
usually
uses
adenoviruses
encode
photosensitive
protein.
The
adenovirus
may
concentrate
in
neural
region.
By
shining
light
on
the
target
nerve
region,
protein
encoded
by
controlled.
Photosensitive
proteins
controlled
can
selectively
allow
ions
inside
outside
cell
membrane
pass
through,
resulting
inhibition
or
activation
effects.
Due
high
precision
minimally
invasive,
optogenetics
has
achieved
good
results
many
fields,
especially
field
of
neuron
functions
circuits.
Significant
advances
have
also
been
made
study
clinical
diseases.
review
focuses
research
neurobiology.
These
include
how
use
cells,
circuits,
treat
diseases
changing
state
We
hoped
this
will
give
comprehensive
understanding
progress
Proceedings of the National Academy of Sciences,
Год журнала:
2022,
Номер
119(13)
Опубликована: Март 23, 2022
Significance
An
influential
idea
in
neuroscience
is
that
neural
circuits
do
not
only
passively
process
sensory
information
but
rather
actively
compare
them
with
predictions
thereof.
A
core
element
of
this
comparison
prediction-error
neurons,
the
activity
which
changes
upon
mismatches
between
actual
and
predicted
stimuli.
While
it
has
been
shown
these
neurons
come
different
variants,
largely
unresolved
how
they
are
simultaneously
formed
shaped
by
highly
interconnected
networks.
By
using
a
computational
model,
we
study
circuit-level
mechanisms
give
rise
to
variants
neurons.
Our
results
shed
light
on
formation,
refinement,
robustness
circuits,
an
important
step
toward
better
understanding
predictive
processing.
Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Март 26, 2024
Abstract
For
generations
researchers
have
been
observing
the
dynamic
processes
of
life
through
lens
a
microscope.
This
has
offered
tremendous
insights
into
biological
phenomena
that
span
multiple
orders
time-
and
length-scales
ranging
from
pure
magic
molecular
reorganization
at
membrane
immune
cells,
to
cell
migration
differentiation
during
development
or
wound
healing.
Standard
fluorescence
microscopy
techniques
offer
glimpses
such
in
vitro,
however,
when
applied
intact
systems,
they
are
challenged
by
reduced
signal
strengths
signal-to-noise
ratios
result
deeper
imaging.
As
remedy,
two-photon
excitation
(TPE)
takes
special
place,
because
it
allows
us
investigate
vivo,
their
natural
environment,
even
living
animal.
Here,
we
review
fundamental
principles
underlying
TPE
aimed
basic
advanced
users
interested
adopting
for
intravital
We
focus
on
applications
neurobiology,
present
current
trends
towards
faster,
wider
imaging,
discuss
combination
with
photon
counting
technologies
metabolic
imaging
spectroscopy,
as
well
highlight
outstanding
issues
drawbacks
application
these
methodologies.