Transcriptomic and Proteomic Analysis of Monkeypox Virus A5L-Expressing HEK293T Cells
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(1), С. 398 - 398
Опубликована: Янв. 5, 2025
Monkeypox
(MPOX)
is
a
zoonotic
viral
disease
caused
by
the
virus
(MPXV),
which
has
become
most
significant
public
health
threat
within
Orthopoxvirus
genus
since
eradication
of
Variola
(VARV).
Despite
extensive
attention
MPXV
garnered,
little
known
about
its
clinical
manifestations
in
humans.
In
this
study,
high-throughput
RNA
sequencing
(RNA-seq)
and
liquid
chromatography-tandem
mass
spectrometry
(LC-MS/MS)
approach
was
employed
to
investigate
transcriptional
metabolic
responses
HEK293T
cells
A5L
protein.
RNA-seq
analysis
identified
total
1473
differentially
expressed
genes
(DEGs),
comprising
911
upregulated
562
downregulated
genes.
Additionally,
LC-MS/MS
revealed
185
cellular
proteins
with
significantly
altered
abundance
ratios
that
interact
Here,
we
perform
Gene
Ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
transcriptome
proteome
signatures
A5L-expressing
gain
insights
into
proteins-host
interplay.
Transcriptomic
transfection
protein
modulated
primarily
associated
cell
cycle,
ribosome,
DNA
replication.
Proteomic
indicated
predominantly
interacted
host
ribosomal
cytoskeletal
proteins.
The
combination
transcriptomic
proteomic
offers
new
perspectives
for
understanding
interaction
between
pathogens
hosts.
Our
research
emphasizes
role
facilitating
internalization
assembly,
as
well
impact
on
host’s
translation
system.
Язык: Английский
A Historical to Future Perspective of Monkeypox Virus and Future Immunotherapeutics
Опубликована: Ноя. 6, 2024
Monkeypox
virus
(MPXV)
isolation
occurred
in
1958,
with
previously
non–endemic
areas
initially
announced
2003,
the
second
epidemic
occurring
2022.
The
third
announcement
2024
was
confirmed
by
World
Health
Organisation
(WHO).
MPXV
outbreak
found
to
be
a
lineage
derived
from
clade
IIb
named
B1
strain;
however,
between
2023-2024,
I
and
II
strains
are
known
have
sub–lineages
potential
apparence
of
co–circulation.
Historically,
now
extinct
variola
(VARV)
used
occur
two
characterised
types
(minor
/
major),
latter
causal
agent
smallpox
disease
severity.
also
into
clades
(clade
I/II),
further
lineages
similar
differential
infection
fatality
rate
(IFR)
previously.
Existing
therapeutics
were
evaluated
since
1796
considered
this
review
as
prophylactic
or
post–exposure
prophylactics.
These
investigated
through
homology
proteins
both
VARV
eradicated.
This
has
three
objectives.
Firstly,
an
analysis
epidemiology
current
outbreak,
context
other
poxviruses.
encompasses
historical
development
above
two,
whilst
spans
future
cancer
arising
investigational
new
drug
developmental
research
vaccinia
(VACV)
complete
genome
sequencing
1990s.
Язык: Английский