Frontiers in Hematology,
Год журнала:
2023,
Номер
2
Опубликована: Ноя. 16, 2023
Leukemic
B-cells
are
lodged
in
the
bone
marrow
[BM],
a
complex
organ
composed
of
many
cell
types
and
extracellular
matrix.
Determining
how
reciprocal
interactions
between
these
components
regulated
is
critical
to
our
understanding
factors
that
allow
leukemia
cells
survive,
multiply
withstand
treatment.
All
surrounded
by
glycocalyx,
glycan-rich
layer
high
complexity,
which
regulates
such
cell-cell
cell-matrix
interactions.
However,
structure
function
glycan
biomolecules
constitute
this
have
not
been
explored
much
detail.
Gaps
difficult
fill
due
technical
limitations
as
well
fact
composition
BM
health,
disease
aging
static.
This
also
applies
B-lineage
malignancies
develop
or
persist
B-cell
precursor
acute
lymphoblastic
Multiple
Myeloma,
effects
their
In
contrast,
proteomes
transcriptomes
different
human
studied
more
extensively.
A
combination
technologies
now
increasingly
allows
correlations
be
made
expression
glycosyltransferases
structures
lines,
could
extrapolated
RNAseq
data
from
primary
cells.
Glycopeptide
analysis
will
invaluable
providing
details
specific
occupancy
on
glycoproteins,
even
if
only
snapshot
time.
Functional
studies
CD19,
CD138/SDC1
BCMA/TNFRSF17
already
demonstrated
importance
glycosylation.
Additional
using
approaches
likely
find
other
instances
malignant
homeostasis
glycosylation,
lead
identification
new
targets
treat
malignancies.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Янв. 4, 2024
Abstract
N-
glycosylation
is
an
abundant
post-translational
modification
of
most
cell-surface
proteins.
glycans
play
a
crucial
role
in
cellular
functions
like
protein
folding,
localization,
cell–cell
signaling,
and
immune
detection.
As
different
tissue
types
display
glycan
profiles,
changes
compositions
occur
tissue-specific
ways
with
development
disease,
cancer.
However,
no
comparative
atlas
resource
exists
for
documenting
glycome
alterations
across
various
human
types,
particularly
comparing
normal
cancerous
tissues.
In
order
to
study
broad
range
N
-glycomes,
-glycan
targeted
MALDI
imaging
mass
spectrometry
was
applied
custom
formalin-fixed
paraffin-embedded
microarrays.
These
encompassed
fifteen
including
bladder,
breast,
cervix,
colon,
esophagus,
gastric,
kidney,
liver,
lung,
pancreas,
prostate,
sarcoma,
skin,
thyroid,
uterus.
Each
array
contained
both
tumor
cores
from
the
same
pathology
block,
selected
by
pathologist,
allowing
more
in-depth
comparisons
differences
between
types.
Using
established
MALDI-IMS
workflows
existing
databases,
present
each
core
were
spatially
profiled
peak
intensity
data
compiled
analyses.
Further
structural
information
determined
fucosylation
using
endoglycosidase
F3,
differentiation
sialic
acid
linkages
through
stabilization
chemistry.
Glycan
compared
oligomannose
levels,
branching
complexity,
presence
bisecting
acetylglucosamine,
fucosylation,
sialylation.
Collectively,
our
research
identified
that
significantly
increased
and/or
decreased
relative
abundance
cancer
type.
This
offers
valuable
on
wide
scale
tissues,
serving
as
reference
future
studies
potential
diagnostic
applications
MALDI-IMS.
Genes,
Год журнала:
2023,
Номер
14(10), С. 1886 - 1886
Опубликована: Сен. 28, 2023
In
the
brain,
cell
adhesion
molecules
(CAMs)
are
critical
for
neurite
outgrowth,
axonal
fasciculation,
neuronal
survival
and
migration,
synapse
formation
maintenance.
Among
CAMs,
IgLON
family
comprises
five
members:
Opioid
Binding
Protein/Cell
Adhesion
Molecule
Like
(OPCML
or
OBCAM),
Limbic
System
Associated
Membrane
Protein
(LSAMP),
neurotrimin
(NTM),
Neuronal
Growth
Regulator
1
(NEGR1),
IgLON5.
IgLONs
exhibit
three
N-terminal
C2
immunoglobulin
domains;
several
glycosylation
sites;
a
glycosylphosphatidylinositol
anchoring
to
membrane.
Interactions
as
homo-
heterodimers
in
cis
trans,
well
binding
other
molecules,
appear
their
functions.
Shedding
by
metalloproteases
generates
soluble
factors
interacting
with
cellular
receptors
activating
signal
transduction.
The
aim
of
this
review
was
analyse
available
data
implicating
role
neuropsychiatric
disorders.
Starting
from
identification
pathological
antibodies
against
IgLON5
an
autoimmune
neurodegenerative
disease
poorly
understood
mechanism
action,
accumulating
evidence
links
disorders,
albeit
still
undefined
mechanisms
which
will
require
future
thorough
investigations.
Molecular Neurobiology,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 3, 2024
Perineuronal
nets
(PNNs)
are
a
type
of
extracellular
matrix
(ECM)
that
play
significant
role
in
synaptic
activity
and
plasticity
interneurons
health
disease.
We
researched
PNNs'
regional
laminar
representation
molecular
composition
using
immunohistochemistry
transcriptome
analysis
Brodmann
areas
(BA)
9,
14r,
24
25
human
postmortem
brains
aged
13-82
years.
The
numbers
VCAN-
NCAN-expressing
PNNs,
relative
to
the
total
number
neurons,
were
highest
cortical
layers
I
VI
while
WFA-binding
(WFA
Communications Biology,
Год журнала:
2025,
Номер
8(1)
Опубликована: Март 27, 2025
Generating
condition-specific
metabolic
models
by
mapping
gene
expression
data
to
genome-scale
(GEMs)
is
a
routine
approach
elucidate
disease
mechanisms
from
perspective.
On
the
other
hand,
integrating
variants
that
perturb
enzyme
functionality
same
RNA-seq
may
enhance
GEM
accuracy,
offering
insights
into
genome-wide
pathology.
Our
study
pioneers
extraction
of
both
transcriptomic
and
genomic
reconstruct
personalized
models.
We
map
genes
with
significantly
higher
load
pathogenic
in
Alzheimer's
(AD)
onto
human
together
data.
Comparative
analysis
resulting
patient
control
shows
enhanced
accuracy
detecting
AD-associated
pathways
compared
case
where
only
mapped
on
GEM.
Besides,
several
otherwise
would-be
missed
are
annotated
AD
considering
effect
variants.
The
authors
used
iMAT
algorithm
associated
Disease
(GEMs).
Chemical Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
glycoPATH
integrates
RNAseq
transcriptomic
and
LC-MS/MS
glycomic
data,
providing
a
platform
to
identify
genes
implicated
in
diseases
involving
N
-glycan
biosynthesis
enabling
the
development
of
targeted
therapeutics
for
these
pathways.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(10), С. 4699 - 4699
Опубликована: Май 14, 2025
Aging
and
aging-related
neurodegenerative
disorders,
such
as
Alzheimer's
disease,
are
characterized
by
chronic
inflammation
that
progressively
damages
nervous
tissue
within
the
central
system
(CNS).
In
addition
to
cytokines,
lectin-like
carbohydrate
recognition
molecules
play
a
critical
role
in
modifying
cellular
communication
during
inflammation.
Among
these,
galectins-particularly
anti-inflammatory
galectin-1
pro-inflammatory
galectin-3-stand
out
due
their
immunological
functions
specificity
for
N-acetyllactosamine
structures.
Almost
every
cell
type
CNS
can
express
recognize
galectins,
influencing
various
essential
functions.
N-acetyllactosamines,
ligand
structures
recognized
found
beneath
sialylated
termini
protein-linked
oligosaccharides.
During
aging,
oligosaccharide
become
shorter,
exposing
N-acetyllactosamines
on
protein
surfaces,
which
enhances
availability
binding
sites
galectins.
Genomic
studies
indicate
number
of
galectin-1-
galectin-3-expressing
microglial
cells
increases
with
age-
or
age-related
disease
(Alzheimer's
disease),
reflecting
an
aging-associated
rise
galectin
concentrations
CNS.
This
increase
parallels
free
N-acetyllactosamine-like
ligands,
suggesting
galectin-N-acetyllactosamine
interactions
gain
prominence
more
significant
disorders.
Understanding
these
molecular
implications
offers
potential
avenues
targeted
therapeutic
strategies
combating
neurodegeneration.
