Rootstock Selection for Resisting Cucumber Fusarium Wilt in Hainan and Corresponding Transcriptome and Metabolome Analysis
Plants,
Год журнала:
2025,
Номер
14(3), С. 359 - 359
Опубликована: Янв. 24, 2025
Soilborne
diseases
are
important
problems
in
modern
agricultural
production.
Fusarium
oxysporum
f.
sp.
cucumerinum
(FOC)
is
one
of
the
predominant
soilborne
pathogens
threatening
cucumber
cultivation,
especially
Hainan,
China.
This
study
assessed
FOC-resistant
rootstocks
using
incidence
rate,
disease
severity
index
(DSI),
and
area
under
curve
(AUDRC),
revealing
“JinJiaZhen
(Mc-4)”
as
resistant
“JinGangZhuan
1901
(Mc-18)”
susceptible.
Comprehensive
transcriptome
metabolome
analyses
were
conducted
to
investigate
defense
mechanisms
these
rootstocks,
key
pathways,
such
mitogen-activated
protein
kinase
(MAPK)
signaling
pathway,
starch
sucrose
metabolism,
phenylpropanoid
biosynthesis,
which
crucial
for
plant
resistance.
Additionally,
compared
resistance
two
other
Mc-4
Mc-18,
against
FOC
infection
through
transcriptomic
metabolomic
analyses.
exhibited
a
higher
number
differentially
expressed
genes
(DEGs)
related
biosynthesis
Mc-18.
Untargeted
metabolomics
identified
4093
metabolites,
with
isoquinoline
alkaloid
porphyrin
metabolism
primary
annotated
pathways.
On
sixth
day
post-inoculation,
when
DEGs
accumulated
metabolites
(DAMs)
was
highest,
emerged
pathway
Mc-4,
37
8
DAMs
identified.
Notably,
showed
upregulated
expression
encoding
enzymes
involved
increased
accumulation
coniferyl-aldehyde,
coniferyl
alcohol,
acetate.
These
findings
highlight
differential
between
sensitive
provide
insights
into
plant–pathogen
interactions.
study’s
results
will
contribute
development
better
disease-free
varieties,
promoting
sustainable
agriculture.
Язык: Английский
Enhancing saline-alkali tolerance in cucumber seedlings: The role of exogenous melatonin in redox homeostasis and stomatal function
Shuchao Huang,
Peng Wu,
Xiting Yang
и другие.
Plant Stress,
Год журнала:
2025,
Номер
unknown, С. 100789 - 100789
Опубликована: Март 1, 2025
Язык: Английский
Genome-wide identification of the CYP82 gene family in cucumber and functional characterization of CsCYP82D102 in regulating resistance to powdery mildew
PeerJ,
Год журнала:
2024,
Номер
12, С. e17162 - e17162
Опубликована: Март 28, 2024
The
cytochrome
P450
(CYP450)
gene
family
plays
a
vital
role
in
basic
metabolism,
hormone
signaling,
and
enhances
plant
resistance
to
stress.
Among
them,
the
CYP82
is
primarily
found
dicots,
they
are
typically
activated
response
various
specific
environmental
stresses.
Nevertheless,
their
roles
remain
considerably
obscure,
particularly
within
context
of
cucumber.
In
present
study,
12
subfamily
genes
were
identified
cucumber
genome.
Bioinformatics
analysis
included
structure,
conserved
motif,
cis-acting
promoter
element,
so
on.
Subcellular
localization
predicted
that
all
located
endoplasmic
reticulum.
results
cis
element
showed
CYP82s
may
significantly
affect
stress,
hormones,
light
exposure.
Expression
patterns
characterized
by
mining
available
RNA-seq
data
followed
qRT-PCR
(quantitative
real-time
polymerase
chain
reaction)
analysis.
Members
display
expression
profiles
different
tissues,
PM
abiotic
stresses
this
CsCYP82D102
,
member
family,
was
investigated.
upregulation
powdery
mildew
(PM)
infection
treatment
with
methyl
jasmonate
(MeJA)
or
salicylic
acid
(SA)
demonstrated.
Further
research
transgenic
plants
overexpressing
heightened
against
PM.
Wild-type
(WT)
leaves
exhibited
average
lesion
areas
approximately
29.7%
at
7
dpi
upon
inoculation.
contrast,
two
independent
overexpression
lines
(OE#1
OE#3)
displayed
reduced
necrotic
areas,
13.4%
5.7%.
Additionally,
enhanced
associated
elevated
related
SA/MeJA
signaling
pathway
plants.
This
study
provides
theoretical
basis
for
further
on
biological
functions
Язык: Английский
Synergistic role of rootstock and grafting in boosting growth, yield, and quality of cucumber cultivation
Plant Science Today,
Год журнала:
2024,
Номер
11(sp4)
Опубликована: Дек. 28, 2024
Cucumber
(Cucumis
sativus
L.)
is
a
widely
grown
and
highly
valued
cucurbitaceous
vegetable
crop,
commanding
premium
prices
in
the
market.
However,
intensive
cultivation
monocropping
practices
have
led
to
significant
challenges
cucumber
production,
particularly
greenhouses,
due
root-knot
nematode
soil-borne
diseases.
Grafting
has
emerged
as
an
alternative
approach
boost
abiotic
stress
tolerance
mitigate
root
diseases
caused
by
pathogens,
thereby
improving
crop
productivity.
A
study
was
conducted
during
2023-2024
on
farmer’s
field
explore
interactive
effects
of
various
grafting
techniques
hybrid
scion
onto
local
rootstocks.
The
experiment
evaluated
performance
Emistar,
grafted
four
rootstocks:
fig-leaf
gourd
(Cucurbita
ficifolia),
pumpkin
pepo),
ash
(Benincasa
hispida),
bottle
(Lagenaria
siceraria).
Two
techniques-hole
insertion
splice
grafting,
were
tested
using
different
scion-rootstock
combinations.
growth,
yield,
fruit
quality
under
controlled
environmental
conditions.
results
indicated
that
rootstock
outperformed
other
rootstocks,
when
hole
techniques.
This
combination
achieved
earliest
flowering
(26.33
days),
longest
plant
growth
(455.77
cm),
highest
yield
(8.07
kg).
In
contrast,
resulted
slower
lower
yields.
Additionally,
produced
superior
characteristics,
including
fruits
(16.43
largest
diameter
(9.1cm),
weight
(147.24
g),
TSS
content
(2.85ºBrix).
Correlation
principal
component
analysis
(PCA)
highlighted
strong
positive
relationships
between
traits,
emphasizing
superiority
for
grafting.
showed
vigorous
rootstocks
like
can
significantly
enhance
through
improved
nutrient
uptake,
hormone
translocation,
optimized
Язык: Английский
Influence of cucurbitaceae rootstocks on growth, yield and quality of grafted cucumber
Journal of Horticultural Science & Technology,
Год журнала:
2024,
Номер
unknown, С. 38 - 42
Опубликована: Июнь 30, 2024
Cucumber
(Cucumis
sativus
L.)
is
among
the
most
widely
cultivated
vegetables
globally
and
ranks
top
second
commercially
essential
vegetables.
However,
its
commercial
greenhouse
production
constrained
by
various
biotic
abiotic
stresses
incompatibility
between
scions
rootstocks.
This
study
evaluated
qualitative
quantitative
attributes
of
cucumber
grafted
onto
different
cucurbit
The
variety
‘Yayla’
was
used
as
scion,
while
four
rootstocks
(37100,
37103,
pumpkin,
bottle
gourd)
were
tested.
Wedge
grafting
performed
15
days
after
seedling
emergence,
parameters
recorded,
including
plant
height,
number
leaves
per
plant,
leaf
area,
fruit
length,
diameter,
weight,
total
soluble
solids
(TSS)
pH
juice.
results
demonstrated
that
on
rootstock
37100
exhibited
superior
performance,
with
highest
values
recorded
for
height
(86.25
mm),
area
(19.66
cm²),
diameter
(33.46
weight
(93.03
g),
length
(16.91
cm),
TSS
(3.11
°Brix),
(6.57).
Conversely,
lowest
these
observed
in
plants
pumpkin
gourd
concluded
significantly
improves
growth,
yield,
quality
attributes,
making
it
a
promising
approach
enhancing
production.
Язык: Английский