Genomic Insights into SARS-CoV-2 Evolution: Nucleocapsid Gene Mutations in West Sumatra, Indonesia DOI Open Access

Linosefa Linosef,

Hasmiwati Hasmiwati,

Jamsari Jamsari

и другие.

International Journal of Research and Review, Год журнала: 2024, Номер 11(5), С. 607 - 613

Опубликована: Июнь 10, 2024

The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic has necessitated a comprehensive understanding of viral mutations, particularly in the nucleocapsid protein, which plays crucial role virus's life cycle and interaction with host immune system. This study aims to analyze (N) gene mutations SARS-CoV-2 variants isolated from West Sumatra during pandemic. A dataset 352 sequences local COVID-19 cases was examined identify N assess their prevalence across different variants. primary identified were R203K G204R ancestral Omicron However, D63G D377Y mutation is present Delta variant. These are implicated enhancing infectivity potential evasion. Notably, variant showed additional deletions (P13L, E31-, R32-, S33-, S413R) that harm protein expansion for structural stability functionality protein. conservation specific suggests fundamental replication interaction. Variant-specific may represent adaptive responses pressure or other environmental factors, significant implications transmissibility pathogenicity. Understanding impact provides critical insights into evolutionary dynamics aids developing effective vaccines therapeutics. Continued surveillance detailed functional studies essential manage prepare future outbreaks. Keywords: SARS-CoV-2, gene, evolution.

Язык: Английский

Diverse effects of coronavirus-defective viral genomes on the synthesis of IFNβ and ISG15 mRNAs and coronavirus replication DOI Creative Commons

Hsuan-Wei Hsu,

L.A. Chang,

Chun-Chun Yang

и другие.

Virology Journal, Год журнала: 2025, Номер 22(1)

Опубликована: Фев. 14, 2025

The mechanism by which coronavirus-defective viral genomes (DVGs) affect coronavirus and host cells during infection remains unclear. A variety of DVGs with different RNA structures can be synthesized from coronavirus-infected cells, these also encode proteins. Consequently, in the present study, we first dissected effects individual on synthesis IFNβ ISG15 mRNAs at RNA, protein combined levels, then examined whether coronavirus-DVGs have replication both individually collectively under conditions. To dissect DVG 2.2 5.1, were previously identified their mutants constructed followed transfection. Western blot RT‒qPCR used to detect quantify mRNAs, respectively. conditions, naturally occurring selected transfection after or before hemagglutination assay. These results suggested that (i) levels (ii) inhibit least partly through interferon signaling (iii) Coronavirus regulated diverse coronavirus-derived innate immunity. Such regulation may contribute pathogenesis coronavirus. populations ability are expected potential resources for identification antivirals level combination, methods current study as a platform this purpose.

Язык: Английский

Процитировано

0
A glimpse into viral warfare: decoding the intriguing role of highly pathogenic coronavirus proteins in apoptosis regulation DOI Creative Commons

Leyi Cheng,

Yajuan Rui, Yanpu Wang

и другие.

Journal of Biomedical Science, Год журнала: 2024, Номер 31(1)

Опубликована: Июль 13, 2024

Coronaviruses employ various strategies for survival, among which the activation of endogenous or exogenous apoptosis stands out, with viral proteins playing a pivotal role. Notably, highly pathogenic coronaviruses such as SARS-CoV-2, SARS-CoV, and MERS-CoV exhibit greater array non-structural compared to low-pathogenic strains, facilitating their ability induce via multiple pathways. Moreover, these are adept at dampening host immune responses, thereby bolstering replication persistence. This review delves into intricate interplay between apoptosis, systematically elucidating molecular mechanisms underpinning induction by proteins. Furthermore, it explores potential therapeutic avenues stemming from inhibition antiviral agents utilization apoptosis-inducing modalities. These insights not only shed light on pathogenesis but also offer novel perspectives cancer therapy.

Язык: Английский

Процитировано

3
Detrimental Effects of Anti-Nucleocapsid Antibodies in SARS-CoV-2 Infection, Reinfection, and the Post-Acute Sequelae of COVID-19 DOI Creative Commons

Emi E. Nakayama,

Tatsuo Shioda

Pathogens, Год журнала: 2024, Номер 13(12), С. 1109 - 1109

Опубликована: Дек. 15, 2024

Antibody-dependent enhancement (ADE) is a phenomenon in which antibodies enhance subsequent viral infections rather than preventing them. Sub-optimal levels of neutralizing individuals infected with dengue virus are known to be associated severe disease upon reinfection different serotype. For Severe Acute Respiratory Syndrome Coronavirus type-2 infection, three types ADE have been proposed: (1) Fc receptor-dependent infection cells expressing receptors, such as macrophages by anti-spike antibodies, (2) receptor-independent epithelial and (3) cytokine production anti-nucleocapsid antibodies. This review focuses on the induced examining its potential role COVID-19 during contribution post-acute sequelae COVID-19, i.e., prolonged symptoms lasting at least months after acute phase disease. We also discuss protective effects recently identified that neutralize Omicron variants.

Язык: Английский

Процитировано

2
Genomic Insights into SARS-CoV-2 Evolution: Nucleocapsid Gene Mutations in West Sumatra, Indonesia DOI Open Access

Linosefa Linosef,

Hasmiwati Hasmiwati,

Jamsari Jamsari

и другие.

International Journal of Research and Review, Год журнала: 2024, Номер 11(5), С. 607 - 613

Опубликована: Июнь 10, 2024

The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic has necessitated a comprehensive understanding of viral mutations, particularly in the nucleocapsid protein, which plays crucial role virus's life cycle and interaction with host immune system. This study aims to analyze (N) gene mutations SARS-CoV-2 variants isolated from West Sumatra during pandemic. A dataset 352 sequences local COVID-19 cases was examined identify N assess their prevalence across different variants. primary identified were R203K G204R ancestral Omicron However, D63G D377Y mutation is present Delta variant. These are implicated enhancing infectivity potential evasion. Notably, variant showed additional deletions (P13L, E31-, R32-, S33-, S413R) that harm protein expansion for structural stability functionality protein. conservation specific suggests fundamental replication interaction. Variant-specific may represent adaptive responses pressure or other environmental factors, significant implications transmissibility pathogenicity. Understanding impact provides critical insights into evolutionary dynamics aids developing effective vaccines therapeutics. Continued surveillance detailed functional studies essential manage prepare future outbreaks. Keywords: SARS-CoV-2, gene, evolution.

Язык: Английский

Процитировано

0