Microbial flora of the respiratory tract and skin of artisanal municipal solid waste handlers in Aba, Abia State, Nigeria

Access Microbiology, Год журнала: 2025, Номер 7(1)

Опубликована: Янв. 1, 2025

Municipal solid waste handling carries occupational risk for handlers due to exposure diverse microorganisms and hazardous substances that cause respiratory skin infections. A cross-sectional study was carried out, 150 respondents were recruited using a simple random sampling technique. The sociodemographic characteristics, health-related complaints, health-seeking behaviour the bacterial fungal microflora of tract artisanal municipal some controls in Aba, Nigeria, determined mixed methods research design, involving use interviewer-administered structured questionnaires conventional culture techniques. We analysed data IBM SPSS version 25. results are presented tables as frequencies percentages. majority Aba male (95%). Their mean age is 30 years, group 21–40 years constitutes highest proportion (65%) workforce. Health-related complaints higher (95%) among compared control subjects (4%). findings show high prevalence (57%), eye (34%) (87%) workers, 6%, 0% 2%, respectively, subjects. Seventy-eight (78%) indulge self-medication via over-the-counter (OTC) drugs; 17 (17%) access diagnostic laboratories only 4 (4%) visit hospitals treatment, subjects, who recorded 1 (2%) OTC drugs, 46 (92%) 2 (4 %) hospitals. Acquisition (15%) (3%) personal protective equipment (PPE) very low amongst handlers. total 704 isolates 191 organisms isolated from Among handlers, percentage distribution bacteria almost same at both sites: tract, 241 (49.9%) skin, 242 (50.1%), subjects’ 105 (47.5%) 116 (52.5%), which showed slight difference between sites. S. epidermidis (24%) B. cereus (13%) predominant whereas aureus (29%) (19%) predominated Similarly, (34.3%) (20.9%) while (37.9%) (18.1%) Candida spp. most fungus (81.4%) (42.9%) well (85%) controls. presence fungi increased proportions may be attributed through direct contact with waste, inhalation organic dust laden biological agents poor working conditions Health education improvements necessary mitigate challenges

Язык: Английский

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Klebsiella aerogenes ST117 causing folliculitis in men having sex with men, Belgium, February 2025

Eurosurveillance, Год журнала: 2025, Номер 30(20)

Опубликована: Май 22, 2025

Klebsiella aerogenes has recently been reported as a causative agent of folliculitis in men who have sex with (MSM). We present four cases MSM diagnosed Brussels, Belgium. Patients were aged between 25 and 50 years, all infected by single multilocus sequence type (ST117) strain. This strain carried the yersiniabactin siderophore genes. Following preliminary treatment sulfamethoxazole−trimethoprim for 7−14 days, patients experienced recurrence symptoms, necessitating an extended therapeutic regimen.

Язык: Английский

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Interplay between porin deficiency, fitness, and virulence in carbapenem-non-susceptible Pseudomonas aeruginosa and Enterobacteriaceae

PLoS Pathogens, Год журнала: 2025, Номер 21(2), С. e1012902 - e1012902

Опубликована: Фев. 7, 2025

The increasing resistance of Gram-negative bacteria to last resort antibiotics, such as carbapenems, is particularly concern it a significant cause global health threat. In this context, there an urgent need for better understanding underlying mechanisms leading antimicrobial in order limit its diffusion and develop new therapeutic strategies. review, we focus on the specific role porins carbapenem-resistance Enterobacteriaceae Pseudomonas aeruginosa , which are major human pathogens. Porins outer membrane proteins, play key bacterial permeability allow nutrients enter toxic waste leave. However, these channels also “Achilles’ heel” antibiotics can pass through them reach their target kill bacteria. After describing normal structures pathways regulating expression porins, discuss strategies implemented by access carbapenems cytoplasmic target. We further examine real impact changes susceptibility. Finally, decipher what effect fitness virulence. Our goal integrate all findings give overview how modify face antibiotic selective pressure trying not induce cost.

Язык: Английский

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In Silico Structural and Functional Characterization of Hypothetical Protein WP_000477209.1 from Hypervirulent Klebsiella aerogenes G7 Reveals a Potential Epigenetic Regulator

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Май 20, 2025

Hypothetical proteins (HPs) represent a substantial portion of prokaryotic genomes and may play critical roles in virulence, antibiotic resistance, epigenetic regulation. Klebsiella aerogenes strain G7, hypervirulent multidrug-resistant isolate belonging to the ST93 lineage, contains numerous uncharacterized that could serve as potential therapeutic targets. In this analysis, we performed comprehensive silico analysis structural functional characteristics hypothetical protein WP_000477209.1, selected from pool 472 HPs based on its conserved domain architecture predicted relevance. We evaluated physicochemical properties, subcellular localization, features using tools such ProtParam, CELLO, InterProScan, HHpred, PSIPRED, YASARA. The 294-amino-acid was be stable, hydrophilic cytoplasmic protein, with molecular weight 30.87 kDa theoretical pI 8.98. Domain indicated presence DpnI_C superfamily domain, associated Dam-replacing endonucleases are involved DNA methylation restriction-modification systems. Homology modeling produced high-confidence tertiary structure, 93.2% residues located favored regions Ramachandran plot, corroborated by strong QMEAN Verify3D scores. PrankWeb identified four ligand-binding sites, VICMpred suggested possible virulence-associated functions. Our findings suggest WP_000477209.1 likely acts an regulator stands out promising candidate for further experimental validation drug discovery. This enhances annotation K. aerogenes G7 proteome emphasizes significance characterizing pathogenic bacteria.

Язык: Английский

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Population genomics uncovers global distribution, antimicrobial resistance, and virulence genes of the opportunistic pathogen Klebsiella aerogenes

Cell Reports, Год журнала: 2024, Номер 43(8), С. 114602 - 114602

Опубликована: Авг. 1, 2024

Klebsiella aerogenes is an understudied and clinically important pathogen. We therefore investigate its population structure by genome analysis aligned with metadata. sequence 130 non-duplicated K. clinical isolates identify two inter-patient transmission events. then retrieve all publicly available genomes (n = 1,026, accessed January 1, 2023) analyze them our genomes. develop a core-genome multi-locus sequence-typing scheme. find that species complex comprising four phylogroups undergoing evolutionary divergence, likely forming three species. delineate remarkable clonal diversity worldwide-distributed carbapenemase-encoding clusters, representing high-risk lineages. uncover has open equipped large arsenal of antimicrobial resistance genes. genetic regions specific for aerogenes, encoding type VI secretion system flagella/chemotaxis motility, respectively, both contributing to the virulence. These results provide much-needed insights into pan-genomes aerogenes.

Язык: Английский

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Clerodendranthus spicatus [Orthosiphon aristatus (Blume) Miq.] maintains uric acid homeostasis via regulating gut microbiota and restrains renal inflammation in hyperuricemic nephropathy

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 25, 2024

The kidney damage caused by the deposition of uric acid in kidneys is urgent need for new treatment drugs due to its complex pathogenesis.

Язык: Английский

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Molecular Epidemiology of Carbapenem-Resistant Klebsiella aerogenes in Japan

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(8), С. 4494 - 4494

Опубликована: Апрель 19, 2024

Information regarding Klebsiella aerogenes haboring carbapenemase in Japan is limited. A comprehensive nationwide survey was conducted from September 2014 to December 2022, and 67 non-duplicate strains of carbapenem-resistant K. were isolated 57 healthcare facilities Japan. Through genetic testing whole-genome sequencing, six found possess carbapenemases, including imipenemase (IMP)-1, IMP-6, New Delhi metallo-β-lactamase (NDM)-1, NDM-5. The strain harboring blaNDM-5 the novel ST709, which belongs clonal complex predominant ST4 China. integron containing blaIMP-1 featured oxacillinase-101 gene, a previously unreported structure, with an IncN4 plasmid type. However, integrons possessing blaIMP-6, most commonly identified, matched those reported domestically pneumoniae, suggesting prevalence identical integrons. Transposons blaNDM are similar or transposon structure Japan, that same type could have been transmitted This investigation analyzed mobile elements, such as transposons, understand spread highlighting growing challenge Enterobacterales underscoring critical need for ongoing surveillance control these pathogens.

Язык: Английский

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Investigation of the Resistance of Klebsiella Bacteria to Antibiotics and the Synthesis of Virulence Factors Isolated from Various Pathogenic Infections

Journal La Lifesci, Год журнала: 2024, Номер 5(4), С. 295 - 310

Опубликована: Авг. 16, 2024

In this study,75 clinical samples, including 32 samples of wounds, fifteen burn 17 sputum and eleven urine were collected from patients afflicted with different infections some hospitals in Baghdad city, during the period October to March 2023. It was found that 22 isolates Klebsiella pneumonia after culturing on MacConkey agar, blood Eosin methylene blue medium, addition studying morphologic appearance, biochemical tests, confirmation diagnosis using VITEKA2 system. The antibiotic sensitivity test showed all (100%) multi-drug resistance 7 antibiotics Cefotaxime, Ampicillin, Chloramphenicol, Gentamicin, Rifampicin, while most sensitive Azithromycin. investigation certain virulence factors bacteria revealed encased a capsule incapable producing hemolysin enzyme, but they able produce urease enzyme form biofilm. produced bacteriocin at rate 12%.

Язык: Английский

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Detection of Bacteria with Potential to Cause Hospital-Associated Infections in a Small-Species Veterinary Hospital in Mexico

Microbiology Research, Год журнала: 2024, Номер 15(3), С. 1758 - 1771

Опубликована: Авг. 31, 2024

Hospital-Associated Infections (HAIs) are caused by microorganisms that not present before patients admitted to healthcare facilities, and usually have multidrug resistance profiles. There is ample information active research in human medicine create preventive control measures, but there been fewer efforts animal medicine, studies only a few countries examining how this problem presents veterinary hospitals. In Mexico, no on the presence of multidrug-resistant bacteria associated with HAIs medicine. Therefore, surfaces inanimate objects equipment university hospital for small species were sampled search potential cause HAIs. After isolation, molecular identification tests carried out. One bacterial strain was found be resistant carbapenems, third-generation cephalosporines, penicillin/β-lactamase inhibitors. Additionally, other susceptible genera identified as nosocomial pathogens humans animals. The confirmed. Further should conducted determine isolate’s origin its relationship reported clinical genotypes. This type study highlights importance epidemiological surveillance need underestimate risk posed microorganisms.

Язык: Английский

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In vivo emergence of resistance to ceftazidime/avibactam through modification of chromosomal AmpC β-lactamase in Klebsiella aerogenes

Antimicrobial Agents and Chemotherapy, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 6, 2024

We describe the

Язык: Английский

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