Mitochondrial oxidative stress, calcium and dynamics in cardiac ischaemia‐reperfusion injury DOI Creative Commons

Emily Rozich,

Ulas Ozkurede,

Shanmugasundaram Pakkiriswami

и другие.

The Journal of Physiology, Год журнала: 2025, Номер unknown

Опубликована: Май 31, 2025

Abstract Ischaemia–reperfusion injury (IRI) is a major cause of cardiomyocyte damage and death from myocardial infarction. Oxidative stress, dysregulated calcium (Ca 2+ ) handling disrupted mitochondrial dynamics are all key factors in IRI can play role cell death. Mitochondria primary source oxidative which generated by electron leak the respiratory chain complexes oxidation accumulated succinate upon reperfusion. The permeability transition pore (mPTP), high conductance channel that forms following reperfusion ischaemic mitochondria, has been implicated reperfusion‐induced Although including Ca overload stress regulate mPTP opening have well characterized, composition still actively investigated. Clinically, complicate treatment Therefore, many possible therapeutics to reduce damaging effects under investigation. Antioxidants, pharmaceutical approaches, postconditioning synthetic polymers investigated for use IRI. Still, these interest shown mixed evidence underlying their preclinical clinical research. In this review we discuss our current understanding contributions IRI, where further clarification mechanisms needed identify potential therapeutic targets. image

Язык: Английский

The roles of mitochondria in global and local intracellular calcium signalling DOI
Benjamín Cartes-Saavedra, Arijita Ghosh, György Hajnóczky

и другие.

Nature Reviews Molecular Cell Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Язык: Английский

Процитировано

5

Mitochondrial Calcium Regulation of Cardiac Metabolism in Health and Disease DOI
Enrique Balderas, Sandra Lee, Neeraj Kumar Vasistha

и другие.

Physiology, Год журнала: 2024, Номер 39(5), С. 247 - 268

Опубликована: Май 7, 2024

Oxidative phosphorylation is regulated by mitochondrial calcium (Ca2+) in health and disease. In physiological states, Ca2+ enters via the uniporter rapidly enhances NADH ATP production. However, maintaining homeostasis critical: insufficient impairs stress adaptation, overload can trigger cell death. this review, we delve into recent insights further defining relationship between dynamics oxidative phosphorylation. Our focus on how such regulation affects cardiac function disease, including heart failure, ischemia-reperfusion, arrhythmias, catecholaminergic polymorphic ventricular tachycardia, cardiomyopathies, Barth syndrome, Friedreich's ataxia. Several themes emerge from data. First, critical for fuel substrate selection, metabolite import, matching of supply to demand. Second, regulates both production response reactive oxygen species (ROS), balance its pro- antioxidant effects key it contributes pathological states. Third, exerts localized electron transport chain (ETC), not through traditional allosteric mechanisms but rather indirectly. These hinge specific transporters, as or Na+/Ca2+ exchanger, may be noticeable acutely, contributing differently phenotypes depending whether transporters are acutely chronically modified. Perturbations these novel relationships during disease states either serve compensatory exacerbate impairments Consequently, targeting holds promise a therapeutic strategy variety diseases characterized contractile failure arrhythmias.

Язык: Английский

Процитировано

11

MCU genetically altered mice suggest how mitochondrial Ca2+ regulates metabolism DOI

Jiuzhou Huo,

Jeffery D. Molkentin

Trends in Endocrinology and Metabolism, Год журнала: 2024, Номер 35(10), С. 918 - 928

Опубликована: Апрель 29, 2024

Язык: Английский

Процитировано

4

Early Synapse-Specific Alterations of Photoreceptor Mitochondria in the EAE Mouse Model of Multiple Sclerosis DOI Creative Commons

Dalia Ibrahim,

Karin Schwarz,

Shweta Suiwal

и другие.

Cells, Год журнала: 2025, Номер 14(3), С. 206 - 206

Опубликована: Янв. 30, 2025

Multiple sclerosis (MS) is an inflammatory autoimmune disease of the central nervous system (CNS) linked to many neurological disabilities. The visual frequently impaired in MS. In previous studies, we observed early malfunctions rod photoreceptor ribbon synapses EAE mouse model MS that included alterations synaptic vesicle cycling and disturbances presynaptic Ca2+ homeostasis. Since these events are highly energy-demanding, analyzed whether mitochondria, which play a major role energy metabolism, might be involved at stage. Rod terminals contain single large mitochondrion next ribbon. present study, expression functionally relevant mitochondrial proteins (MIC60, ATP5B, COX1, PINK1, DRP1) by high-resolution qualitative quantitative immunofluorescence microscopy, immunogold electron microscopy Western blot experiments. We decreased mitochondria photoreceptors stage, suggesting dysfunctions important synapse pathology. Interestingly, were strongly compromised EAE, whereas extra-synaptic inner segments remained unchanged, demonstrating functional heterogeneity mitochondria.

Язык: Английский

Процитировано

0

Chimeric Cell Therapy Transfers Healthy Donor Mitochondria in Duchenne Muscular Dystrophy DOI Creative Commons
Maria Siemionow, Katarzyna Bocian,

Katarzyna Bożyk

и другие.

Stem Cell Reviews and Reports, Год журнала: 2024, Номер 20(7), С. 1819 - 1829

Опубликована: Июль 17, 2024

Duchenne muscular dystrophy (DMD) is a severe X-linked disorder characterized by dystrophin gene mutations and mitochondrial dysfunction, leading to progressive muscle weakness premature death of DMD patients. We developed human Dystrophin Expressing Chimeric (DEC) cells, created the fusion myoblasts from normal donors patients, as foundation for DT-DEC01 therapy DMD. Our preclinical studies on mdx mouse models revealed enhanced expression functional improvements in cardiac, respiratory, skeletal muscles after systemic intraosseous DEC administration. The current study explored feasibility transfer within which crucial developing new therapeutic strategies Following staining with MitoTracker Deep Red Green dyes, was assessed Flow cytometry (FACS) confocal microscopy. PEG-mediated healthy (MB

Язык: Английский

Процитировано

3

Effects of aging on calcium channels in skeletal muscle DOI Creative Commons
Maolong Dong, Andrés D. Maturana

Frontiers in Molecular Biosciences, Год журнала: 2025, Номер 12

Опубликована: Март 19, 2025

In skeletal muscle, calcium is not only essential to stimulate and sustain their contractions but also for muscle embryogenesis, regeneration, energy production in mitochondria, fusion. Different ion channels contribute achieving the various functions of muscles. Muscle contraction initiated by releasing from sarcoplasmic reticulum through ryanodine receptor gated mechanically four dihydropyridine receptors T-tubules. The influx store-operated sustains stimulates regeneration. Mitochondrial uniporter allows entry into mitochondria oxidative phosphorylation. Aging alters expression activity these different channels, resulting a reduction force generation regeneration capacity. Regular physical training bioactive molecules nutrients can prevent effects aging on channels. This review focuses current knowledge muscles’

Язык: Английский

Процитировано

0

Identification of VDAC1 as a mitochondria-related target of Duchenne muscular dystrophy based on bioinformatics analysis and in vitro experiments DOI

Wenjuan Wu,

Xueqin Song, Baoguang Li

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 158, С. 114836 - 114836

Опубликована: Май 12, 2025

Язык: Английский

Процитировано

0

Mitochondrial oxidative stress, calcium and dynamics in cardiac ischaemia‐reperfusion injury DOI Creative Commons

Emily Rozich,

Ulas Ozkurede,

Shanmugasundaram Pakkiriswami

и другие.

The Journal of Physiology, Год журнала: 2025, Номер unknown

Опубликована: Май 31, 2025

Abstract Ischaemia–reperfusion injury (IRI) is a major cause of cardiomyocyte damage and death from myocardial infarction. Oxidative stress, dysregulated calcium (Ca 2+ ) handling disrupted mitochondrial dynamics are all key factors in IRI can play role cell death. Mitochondria primary source oxidative which generated by electron leak the respiratory chain complexes oxidation accumulated succinate upon reperfusion. The permeability transition pore (mPTP), high conductance channel that forms following reperfusion ischaemic mitochondria, has been implicated reperfusion‐induced Although including Ca overload stress regulate mPTP opening have well characterized, composition still actively investigated. Clinically, complicate treatment Therefore, many possible therapeutics to reduce damaging effects under investigation. Antioxidants, pharmaceutical approaches, postconditioning synthetic polymers investigated for use IRI. Still, these interest shown mixed evidence underlying their preclinical clinical research. In this review we discuss our current understanding contributions IRI, where further clarification mechanisms needed identify potential therapeutic targets. image

Язык: Английский

Процитировано

0