Abstract
The
coffee
industry
holds
importance,
providing
livelihoods
for
millions
of
farmers
globally
and
playing
a
vital
role
in
the
economies
coffee-producing
countries.
Environmental
conditions
such
as
drought
temperature
fluctuations
can
adversely
affect
quality
yield
crops.Carotenoid
cleavage
oxygenases
(CCO)
enzymes
are
essential
plants
they
help
break
down
carotenoids
contributing
to
growth
stress
resistance.
However,
knowledge
about
CCO
gene
family
Coffee
arabica
was
limited.
In
this
study
identified
21
genes
(C.
arabica)
revealing
two
subfamilies
carotenoid
dioxygenases
(
CCDs)
9-cis-epoxy
NCED
)
through
phylogenic
analysis.
These
exhibited
distribution
patterns
terms
structure,
domains,
motifs.
CaCCO
genes,
comprising
5
16
CCD
were
found
across
chromosomes.
Promoter
sequencing
analysis
revealed
cis-elements
that
likely
interact
with
plant
stress-responsive,
growth-related,
phytohormones,
like
auxin
abscisic
acid.
A
comprehensive
genome-wide
comparison,
between
C.
A.
thaliana
conducted
understand
characteristics
genes.
RTqPCR
data
indicated
CaNCED5,
CaNCED6,
CaNCED12,
CaNCED20
target
involved
leading
increased
crop
yield,
conditions,
limited
water
availability.
This
reveals
CCOs
responding
abiotic
identifies
potential
useful
breeding
stress-resistant
varieties.
BIO Web of Conferences,
Год журнала:
2025,
Номер
155, С. 01015 - 01015
Опубликована: Янв. 1, 2025
Climate
change-induced
heat
stress
poses
a
significant
threat
to
global
soybean
production.
This
study
investigated
the
impact
of
on
growth
and
physiological
parameters
18
Indonesian
varieties
at
seedling
stage.
Treatments
involved
exposing
two-week-old
seedlings
temperatures
25,
30,
35,
40
45°C
for
four
hours
daily
over
consecutive
days.
The
assessed
were
survival
rate,
root
length,
shoot
total
plant
weight,
relative
water
content.
Results
revealed
variability
in
tolerance
among
varieties.
While
all
showed
optimal
25°C,
increasing
from
25
30°C
led
an
increase
growth.
A
critical
threshold
was
observed
between
35
40°C,
where
most
experienced
sharp
performance
decline.
Notably,
Demas
1,
Gepak
Kuning,
Agromulyo
consistently
outperformed
others
even
other
couldn’t
survive,
maintaining
higher
rates
better
under
severe
conditions.
provides
valuable
insights
into
mechanisms
identifies
promising
genetic
resources
breeding
heat-resilient
cultivars
Indonesia.
Our
findings
contribute
development
strategies
mitigate
impacts
climate
change
production,
crucial
ensuring
food
security
face
rising
temperatures.
Agronomy,
Год журнала:
2025,
Номер
15(3), С. 604 - 604
Опубликована: Фев. 28, 2025
The
DIR
gene
family,
which
encodes
Dirigent
proteins,
plays
a
crucial
role
in
plant
development
and
stress
responses.
However,
the
functions
mechanisms
of
this
family
maize
remained
underexplored.
This
study
identified
ZmDIR11,
member
protein
explored
its
drought
tolerance.
ZmDIR11
shared
conserved
regions
with
homologous
proteins
wheat,
rice,
Arabidopsis.
RT-qPCR
analysis
revealed
that
expression
is
upregulated
leaves
under
PEG
stress,
while
subcellular
localization
confirmed
presence
endoplasmic
reticulum.
Virus-induced
silencing
(VIGS)
EMS
mutagenesis
demonstrated
or
mutation
significantly
reduces
tolerance
seedlings,
indicating
positive
regulatory
role.
Silencing
led
to
decreased
growth
parameters
(plant
height,
root
length,
fresh
weight,
dry
chlorophyll
content)
alongside
reduced
antioxidant
capacity,
as
evidenced
by
increased
levels
MDA
ROS
activities
SOD,
CAT,
POD.
Furthermore,
impaired
synthesis
ABA
zeatin,
downregulating
key
genes
(ZmABA1,
ZmNCED3,
ZmSnRK2.6)
zeatin
(ZmIPT4,
ZmCKO5,
ZmCKO4b)
pathways.
Drought-responsive
(ZmRD20,
ZmRD22,
ZmDREB2A)
lignan
biosynthesis
(Zm4CL,
ZmC3H,
ZmCAD1)
are
also
downregulated
mutants.
In
conclusion,
enhances
regulating
synthesis,
metabolism.
provides
new
insights
into
offers
potential
genetic
resource
for
breeding
drought-resistant
varieties.
