Anti-apoptotic effect of umbilical cord cryoextract and its lyophilized form on L929 cells DOI Creative Commons
Anna Kaverinska, В. В. Лазуренко, O.Yu. Zhelezniakov

и другие.

Regulatory Mechanisms in Biosystems, Год журнала: 2024, Номер 15(4), С. 980 - 984

Опубликована: Ноя. 20, 2024

The umbilical cord cryoextract (UCC) and its lyophilized form (LUCC) contain numerous biologically active components, including growth factors, cytokines, other regulatory molecules, which may exert an anti-apoptotic effect on cells. This study aimed to determine whether these samples can reduce apoptosis levels in healthy cells, is particularly relevant scenarios where be induced by chemotherapeutic agents such as doxorubicin. To evaluate the potential protective effects of cryoextracts, L929 cell line treated with doxorubicin, a known inducer, was selected model for assessing properties UCC LUCC. In experiment, cells were cultured standard Dulbecco's Modified Eagle Medium (DMEM) addition Experimental results demonstrated that fetal bovine serum (FBS), UCC, LUCC significantly reduced compared group doxorubicin alone. LUCC, comparable those FBS group, indicating supportive both forms cryoextracts. These findings suggest serve effective reducing apoptosis, maintaining stability their components even post-lyophilization. support use cryoextracts chemotherapy involving mitigate toxic side effects. Further research needed elucidate mechanisms action extracts assess efficacy vivo, open new opportunities application oncology therapy medical fields.

Язык: Английский

Electrospun chitosan/polycaprolactone wound dressing containing henna extract and vitamin C: A natural composite for wound care DOI Open Access

Maryam Sadeghimanesh,

Elahe Poorazizi

Polymer Engineering and Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

Abstract The present study shows an incredibly promising wound dressing material based on electrospun chitosan (CS)/polycaprolactone (PCL) fibers that are enhanced with henna extract (HE) and vitamin C (VitC). incorporation of HE, a traditional medicinal herb known for its healing capabilities, Vit C, powerful antioxidant, into CS takes use CS's natural biocompatibility antibacterial characteristics. This combination requires to generate synergistic effect enhances provides protection against infection. scaffolds were created investigated determine their surface morphology, chemical composition, ability water swelling, strength, kill bacteria. results indicate the inclusion HE substantially improved absorbing water, which is essential encouraging attachment cells maintaining moisture, both critical wounds. Furthermore, containing shown significant efficacy Escherichia coli Staphylococcus aureus , demonstrating capacity prevent According in vitro evaluation, sample 1 wt% VitC exhibited exceptional cell adhesion L929 fibroblast line. introduces innovative has customized mixture substances. approach addresses gaps previous CS/PCL studies, introducing composite advanced care applications. Highlights Electrospun CS/PCL‐HE/VitC potential. improve antimicrobial properties. scaffold demonstrates moisture retention application. activity E. S. aureus. In studies show excellent cells.

Язык: Английский

Процитировано

0

Antibacterial and Biodegradable PLA-based Nanofibers Loaded with Natural Phenolic Monoterpenes for Sustainable Biomedical or Food Application DOI Creative Commons
Lucie Bartošová, Magda Janalí­ková, Jana Sedlaříková

и другие.

New Biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Antibacterial biodegradable PLA-based nanofibers loaded with phenolic monoterpenes - thymol, eugenol, carvacrol, and cinnamaldehyde, were prepared by electrospinning. The effect of bioactive molecule on the surface, thermal, morphological, biological properties has been investigated about potential pharmaceutical food processing applications. Fiber diameters ranged from 320nm for PLA fibrous mat up to 480nm membrane 6% thymol. All active exhibited hydrophobic surfaces a slightly decreasing contact angle after incorporation phenols. Antimicrobial testing proved strong efficiency against Escherichia coli Staphylococcus aureus, depending specific type content compound. A significant biofilm formation reduction was revealed tested microorganisms. Modification fibers molecules did not significantly affect biodegradation kinetics in comparison samples their absence. This study demonstrates high newly developed PLA-based/phenol nanofibrous membranes use as antibacterial antifouling systems applicable wound dressings packaging.

Язык: Английский

Процитировано

0

Blood Plasma, Fibrinogen or Fibrin Biomaterial for the Manufacturing of Skin Tissue-Engineered Products and Other Dermatological Treatments: A Systematic Review DOI Creative Commons
Álvaro Sierra-Sánchez, Raquel Sanabria-de la Torre, Ana Ubago-Rodríguez

и другие.

Journal of Functional Biomaterials, Год журнала: 2025, Номер 16(3), С. 79 - 79

Опубликована: Фев. 22, 2025

The use of blood plasma, fibrinogen or fibrin, a natural biomaterial, has been widely studied for the development different skin tissue-engineered products and other dermatological treatments. This systematic review reports preclinical clinical studies which it alone combined with biomaterials and/or cells treatment several conditions. Following PRISMA 2020 Guidelines, 147 have revealed that this biomaterial as wound dressing monolayer (one cell type) substitute are preferred strategies, mainly excisional surgical wounds. Moreover, plasma is used although its combination such agarose, polyethylene glycol collagen also reported to increase healing potential. However, most 17 reviewed evaluated severely burned patients bilayer (two types) substitute. Although number evaluating increased during last fifteen years, not correlated wide variety studies. Its safety potential proved; however, lack standard model presence approaches meant translation environment still limited. A higher should be carried out in coming years set strategy each disease.

Язык: Английский

Процитировано

0

Fabrication of curcumin‑incorporated poly glycerol sebacate/poly lactide acid (PGS/PLA) hydrogel to enhance full‑thickness wound healing in diabetic rats DOI
Nahed S. Alharthi

Tissue and Cell, Год журнала: 2025, Номер unknown, С. 102856 - 102856

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Chitosan/poly (β‐amino ester) hydrogel by controlled release of Centella Asiatica promoted wound healing through improved collagen expression and antibacterial and anti‐inflammatory properties DOI Open Access

Shima Rashedi,

Parisa Heydari, Anousheh Zargar Kharazi

и другие.

Polymer Engineering and Science, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Abstract Burn wound healing presents several challenges, including impaired collagen synthesis, delayed closure, and disrupted inflammatory responses. This study investigates the potential of a novel UV‐crosslinked hydrogel made chitosan (CS)/poly(β‐amino ester) (PβAE) Centella Asiatica extract (CA) for second‐degree burn treatment. Among various formulations, monomer ratio 1.5:1 5 wt% 2,2‐dimethoxy‐2‐phenyl‐acetophenone DMPA crosslinker concentration were found optimal. The achieved ~74% controlled release CA over 72 h showed significant enhancement in fibroblast viability (123.66 ± 9% at days). Scratch assay demonstrated ~99.5% closure within 24 h. Collagen type I expression was increased by ~1.7‐fold compared with control. Additionally, exhibited remarkable anti‐inflammatory properties, effectively modulating response. It significantly reduced pro‐inflammatory markers such as IL‐6 TNF‐α while simultaneously enhancing levels cytokines, IL‐10 TGF‐β. dual action not only mitigates inflammation but also fosters regenerative environment, further supporting process promoting tissue recovery. antibacterial properties inhibition zones ~19.1 mm E. coli ~12.4 S. aureus . These results suggest that this biocompatible, biodegradable is promising therapeutic candidate wounds. Highlights Developed chitosan/PβAE burns. Optimized releases Boosts viability, migration, 1.7x. Reduces IL‐6, TNF‐α, IL‐10, Strong against aureus.

Язык: Английский

Процитировано

0

Advancements in nanotechnology for biomedical and wearable applications DOI
Nagender Singh, Amandeep Kaur, Amit Madhu

и другие.

Next Materials, Год журнала: 2025, Номер 8, С. 100658 - 100658

Опубликована: Апрель 18, 2025

Язык: Английский

Процитировано

0

Oxygen-generating and antibacterial xanthan gum/PLA aerogels loaded with dexamethasone for potential wound healing DOI

Nika Atelšek Hozjan,

Gabrijela Horvat, Matjaž Finšgar

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143314 - 143314

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Preparation and characterization of an antibacterial CMC/PCL hydrogel films containing CIP/Cur: In vitro and in vivo evaluation of wound healing activity DOI

Maryam Mojahedi,

Parisa Heydari, Anousheh Zargar Kharazi

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 282, С. 136570 - 136570

Опубликована: Окт. 15, 2024

Язык: Английский

Процитировано

2

Anti-apoptotic effect of umbilical cord cryoextract and its lyophilized form on L929 cells DOI Creative Commons
Anna Kaverinska, В. В. Лазуренко, O.Yu. Zhelezniakov

и другие.

Regulatory Mechanisms in Biosystems, Год журнала: 2024, Номер 15(4), С. 980 - 984

Опубликована: Ноя. 20, 2024

The umbilical cord cryoextract (UCC) and its lyophilized form (LUCC) contain numerous biologically active components, including growth factors, cytokines, other regulatory molecules, which may exert an anti-apoptotic effect on cells. This study aimed to determine whether these samples can reduce apoptosis levels in healthy cells, is particularly relevant scenarios where be induced by chemotherapeutic agents such as doxorubicin. To evaluate the potential protective effects of cryoextracts, L929 cell line treated with doxorubicin, a known inducer, was selected model for assessing properties UCC LUCC. In experiment, cells were cultured standard Dulbecco's Modified Eagle Medium (DMEM) addition Experimental results demonstrated that fetal bovine serum (FBS), UCC, LUCC significantly reduced compared group doxorubicin alone. LUCC, comparable those FBS group, indicating supportive both forms cryoextracts. These findings suggest serve effective reducing apoptosis, maintaining stability their components even post-lyophilization. support use cryoextracts chemotherapy involving mitigate toxic side effects. Further research needed elucidate mechanisms action extracts assess efficacy vivo, open new opportunities application oncology therapy medical fields.

Язык: Английский

Процитировано

0