Characterisation of Pseudomonas aeruginosas metal-responsive TonB-dependent transporters DOI Creative Commons

Manon Ferry,

Hortense Ferriz,

Connor Sharp

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Abstract Pseudomonas aeruginosa , a versatile bacterium, relies on several TonB-dependent transporters (TBDTs) for nutrient acquisition (such as iron-siderophore complexes) and adaptation to various environments. While some TBDTs are well characterized, significant number remain unexplored despite their potential role in pathogenicity. In this study, we developed fluorescent reporter plasmids investigate TBDT promoter activity. Initially, characterized activity commonly used laboratory conditions, revealing diverse expression patterns among all TBDTs. Subsequently, classified the into distinct metal-responsive groups based stress-responsive behaviour, shedding light functional roles. Additionally, show that these constructs can be powerful tool siderophore detection. Finally, single cell analysis of during coculture with an enterobactin-producing Klebsiella pneumoniae strain shows homogenous key P. . Our findings provide valuable insights profiles diversity complex reaching from lab media co-culturing conditions.

Язык: Английский

Xenosiderophores: bridging the gap in microbial iron acquisition strategies DOI
Ravinsh Kumar, Ashutosh Singh, Amrita Srivastava

и другие.

World Journal of Microbiology and Biotechnology, Год журнала: 2025, Номер 41(2)

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1
Natural sideromycins and siderophore-conjugated natural products as inspiration for novel antimicrobial agents DOI

Zhiying Miao,

Jing Lin, Wei‐Min Chen

и другие.

European Journal of Medicinal Chemistry, Год журнала: 2025, Номер unknown, С. 117333 - 117333

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Structure of the Outer Membrane Transporter FemA and Its Role in the Uptake of Ferric Dihydro-Aeruginoic Acid and Ferric Aeruginoic Acid in Pseudomonas aeruginosa DOI Creative Commons
Virginie Will, L. Moynié,

Elise Si Ahmed Charrier

и другие.

ACS Chemical Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Iron is essential for bacterial growth, and Pseudomonas aeruginosa synthesizes the siderophores pyochelin (PCH) pyoverdine to acquire it. PCH contains a thiazolidine ring that aids in iron chelation but prone hydrolysis, leading formation of 2-(2-hydroxylphenyl)-thiazole-4-carbaldehyde (IQS). Using mass spectrometry, we demonstrated undergoes hydrolysis oxidation solution, resulting aeruginoic acid (AA). This study used proteomic analyses fluorescent reporters show AA, dihydroaeruginoic (DHA), induce expression femA, gene encoding ferri-mycobactin outer membrane transporter P. aeruginosa. Notably, induction by AA DHA was observed only strains unable produce pyoverdine, suggesting their weaker iron-chelating ability compared pyoverdine. 55Fe uptake assays both AA-Fe DHA-Fe complexes are transported via FemA; however, no PCH-Fe through this transporter. Structural studies revealed FemA able bind AA2-Fe or DHA2-Fe complexes. Key interactions conserved between these two complexes, with specificity primarily driven one siderophore molecules. Interestingly, although noted FemA, also binds similar manner. These findings under moderate deficiency, when produced aeruginosa, degradation products enhance inducing femA facilitating transport FemA. provides new insights into pathogen's strategies homeostasis.

Язык: Английский

Процитировано

0
Characterisation of Pseudomonas aeruginosas metal-responsive TonB-dependent transporters DOI Creative Commons

Manon Ferry,

Hortense Ferriz,

Connor Sharp

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Abstract Pseudomonas aeruginosa , a versatile bacterium, relies on several TonB-dependent transporters (TBDTs) for nutrient acquisition (such as iron-siderophore complexes) and adaptation to various environments. While some TBDTs are well characterized, significant number remain unexplored despite their potential role in pathogenicity. In this study, we developed fluorescent reporter plasmids investigate TBDT promoter activity. Initially, characterized activity commonly used laboratory conditions, revealing diverse expression patterns among all TBDTs. Subsequently, classified the into distinct metal-responsive groups based stress-responsive behaviour, shedding light functional roles. Additionally, show that these constructs can be powerful tool siderophore detection. Finally, single cell analysis of during coculture with an enterobactin-producing Klebsiella pneumoniae strain shows homogenous key P. . Our findings provide valuable insights profiles diversity complex reaching from lab media co-culturing conditions.

Язык: Английский

Процитировано

0