Long-term co-circulation of multiple arboviruses in southeast Australia revealed by xeno-monitoring and viral whole genome sequencing DOI Creative Commons
Carla Júlia da Silva Pessoa Vieira, Michael B. Onn, Martin A. Shivas

и другие.

Virus Evolution, Год журнала: 2024, Номер 10(1), С. 0 - 0

Опубликована: Янв. 1, 2024

Abstract Arbovirus surveillance of wild-caught mosquitoes is an affordable and sensitive means monitoring virus transmission dynamics at various spatial-temporal scales, emergence re-emergence during epidemic interepidemic periods. A variety molecular diagnostics for arbovirus screening (known as xeno-monitoring) are available, but most provide limited information about diversity. Polymerase chain reaction (PCR)-based coupled with RNA sequencing increasingly pipeline integrating complete viral genome into programs. This enables large-scale, high-throughput from diverse samples. We collected in CO2-baited light traps five urban parks Brisbane March 2021 to May 2022. Mosquito pools ≤200 specimens were screened alphaviruses flaviviruses using genus-specific primers reverse transcription quantitative PCR (qRT-PCR). subset virus-positive samples was then processed a mosquito-specific ribosomal depletion method sequenced on the Illumina NextSeq. Overall, 54,670 representing 26 species 382 pools. Thirty detections arboviruses made 28 Twenty these positive further characterized generating 18 full-length genomes. These sequences belonged four medically relevant arboviruses: Barmah Forest, Ross River, Sindbis-like, Stratford viruses. Phylogenetic evolutionary analyses revealed progression lineages over last 100 years, demonstrating that different epidemiological, immunological, processes may actively shape evolution Australian arboviruses. results underscore need more genomic data explore complex pressures acting our findings highlight effectiveness methodology, which can be applied broadly enhance ecological contexts improve understanding dynamics.

Язык: Английский

Long-term co-circulation of multiple arboviruses in southeast Australia revealed by xeno-monitoring and viral whole genome sequencing DOI Creative Commons
Carla Júlia da Silva Pessoa Vieira, Michael B. Onn, Martin A. Shivas

и другие.

Virus Evolution, Год журнала: 2024, Номер 10(1), С. 0 - 0

Опубликована: Янв. 1, 2024

Abstract Arbovirus surveillance of wild-caught mosquitoes is an affordable and sensitive means monitoring virus transmission dynamics at various spatial-temporal scales, emergence re-emergence during epidemic interepidemic periods. A variety molecular diagnostics for arbovirus screening (known as xeno-monitoring) are available, but most provide limited information about diversity. Polymerase chain reaction (PCR)-based coupled with RNA sequencing increasingly pipeline integrating complete viral genome into programs. This enables large-scale, high-throughput from diverse samples. We collected in CO2-baited light traps five urban parks Brisbane March 2021 to May 2022. Mosquito pools ≤200 specimens were screened alphaviruses flaviviruses using genus-specific primers reverse transcription quantitative PCR (qRT-PCR). subset virus-positive samples was then processed a mosquito-specific ribosomal depletion method sequenced on the Illumina NextSeq. Overall, 54,670 representing 26 species 382 pools. Thirty detections arboviruses made 28 Twenty these positive further characterized generating 18 full-length genomes. These sequences belonged four medically relevant arboviruses: Barmah Forest, Ross River, Sindbis-like, Stratford viruses. Phylogenetic evolutionary analyses revealed progression lineages over last 100 years, demonstrating that different epidemiological, immunological, processes may actively shape evolution Australian arboviruses. results underscore need more genomic data explore complex pressures acting our findings highlight effectiveness methodology, which can be applied broadly enhance ecological contexts improve understanding dynamics.

Язык: Английский

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