Salivary microbiome and periodontopathogen/denitrifying bacteria associated with gingivitis and periodontitis in people with type 2-diabetes
F1000Research,
Год журнала:
2025,
Номер
14, С. 297 - 297
Опубликована: Март 14, 2025
Background
Despite
diabetes
mellitus
and
periodontal
diseases
are
mutually
exclusive,
little
is
known
about
particular
types
of
bacteria
that
may
have
exacerbated
the
development
diabetics’
inflammation.
This
study’s
aim
was
to
compare
salivary
microbiomes
individuals
with
type
2
(20–40
years
old)
who
had
gingivitis
or
periodontitis
those
did
not.
Additionally,
we
evaluated
relationship
between
number
periodontopathogens
amount
nitrate-reducing
in
their
microbiome.
Methods
Saliva
collected,
DNA
isolated,
entire
16S
ribosomal
RNA
gene
amplified,
sample
libraries
were
prepared
accordance
Oxford
Nanopore
MinION
Technology
procedure.
The
relative
abundance
bacterial
diversity
saliva
samples
pooled
according
three
groups;
T2DM
patients
without
disease
(G1),
(G2),
(G3),
measured
using
bioinformatic
methods.
relationships
periodontopathic
(Porphyromonas
gingivalis,
Treponema
denticola,
Tannerella
forsythia,
Fusobacterium
spp.)
denitrifying
community
(Haemophilus,
Neisseria,
Rothia,
Veillonella)
assessed.
Results
Alpha-diversity
analysis
revealed,
G1
group
significantly
lower
than
groups
G2
G3
(p<
0.0001).
However,
microbiota
profiles
diabetic
patient
comparable.
Using
receiver
operating
characteristic
(ROC)
analysis,
potential
biomarkers
for
differentiating
discovered.
Areas
under
curve
(AUC)
spp.
Neisseria
found
be
0.94
(p
=
0.43),
while
AUC
P.
gingivalis
Rothia
not
significant
(0.84,
p
0.08).
Conclusion
People
also
exhibit
different
These
features
might
essential
indicators
early
identification
treatment
order
prevent
periodontitis.
Язык: Английский
Glycemic Control, Inflammatory Mediators, and Periodontal Health: A Cross-Sectional Study in Patients with Diabetes
Journal of Clinical Medicine,
Год журнала:
2025,
Номер
14(8), С. 2847 - 2847
Опубликована: Апрель 21, 2025
Background/Objectives:
The
bidirectional
relationship
between
diabetes
mellitus
(DM)
and
periodontal
disease
(PD)
has
garnered
increasing
attention
due
to
shared
inflammatory
mechanisms
mutual
exacerbation.
In
Romania,
despite
a
high
prevalence
of
PD,
integration
oral
health
into
care
remains
limited.
This
study
aimed
investigate
the
association
glycemic
control,
salivary
biomarkers
(IL-1β,
IL-6,
MMP-8),
status
in
diabetic
patients.
Additionally,
it
evaluated
patients’
awareness
risks
their
communication
with
healthcare
providers
regarding
care.
Methods:
A
cross-sectional,
observational
was
conducted
May
December
2024,
involving
79
adult
patients
confirmed
type
1
or
2
DM.
Periodontal
examinations
assessed
probing
pocket
depth
(PPD),
clinical
attachment
level
(CAL),
plaque
index
(PI),
bleeding
on
(BOP).
Salivary
samples
were
collected
quantify
IL-1β,
MMP-8.
Participants
also
completed
structured
questionnaire
symptoms,
hygiene
practices,
diabetes–periodontitis
link.
Correlation
t-test
analyses
used
explore
associations
clinical,
biochemical,
self-reported
variables.
Results:
Most
participants
had
advanced
periodontitis
(65.8%
Stage
IV;
72.2%
Grade
C).
IL-1β
IL-6
positively
correlated
(r
=
0.34,
p
<
0.01),
while
MMP-8
PI
0.28)
BOP
0.26).
Inflammatory
markers
showed
weak
correlation
HbA1c.
Notably,
higher
knowledge
reported
worse
indices,
suggesting
increased
symptom
rather
than
preventive
effectiveness.
Conclusions:
reinforces
link
DM
PD
highlights
need
for
integrated
models.
screening
education
should
be
embedded
within
management,
particularly
high-risk
populations.
Язык: Английский