Cirsiliol Alleviates Experimental Inflammatory Bowel Disease via Restoration of Intestinal Barrier Integrity and Inhibition of NF-κB and MAPK Pathways DOI
Juan Gao, Haiyang Li, Zhenquan Wang

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

Abstract Inflammatory bowel disease (IBD) is a chronic gastrointestinal disorder characterized by intestinal inflammation and barrier dysfunction. This study investigated the therapeutic potential of Cirsiliol, natural flavonoid, in DNBS-induced murine colitis model..Mice were randomly divided into control, model, Cirsiliol treatment groups (10 mg/kg 30 mg/kg). Disease activity was assessed through body weight, colon length, index (DAI). Molecular mechanisms evaluated using immunohistochemistry, Western blot, biochemical assays. significantly ameliorated symptoms, reducing weight loss DAI scores while improving length. Mechanistically, suppressed pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), enhanced antioxidant defenses (increased GSH, decreased MDA), restored tight junction protein expression (Claudin-1, Occludin, E-cadherin). Furthermore, it modulated apoptosis-related proteins (reduced Bax, Caspase-3; increased Bcl-2) promoted epithelial migration.

Язык: Английский

Cirsiliol Alleviates Experimental Inflammatory Bowel Disease via Restoration of Intestinal Barrier Integrity and Inhibition of NF-κB and MAPK Pathways DOI
Juan Gao, Haiyang Li, Zhenquan Wang

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

Abstract Inflammatory bowel disease (IBD) is a chronic gastrointestinal disorder characterized by intestinal inflammation and barrier dysfunction. This study investigated the therapeutic potential of Cirsiliol, natural flavonoid, in DNBS-induced murine colitis model..Mice were randomly divided into control, model, Cirsiliol treatment groups (10 mg/kg 30 mg/kg). Disease activity was assessed through body weight, colon length, index (DAI). Molecular mechanisms evaluated using immunohistochemistry, Western blot, biochemical assays. significantly ameliorated symptoms, reducing weight loss DAI scores while improving length. Mechanistically, suppressed pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), enhanced antioxidant defenses (increased GSH, decreased MDA), restored tight junction protein expression (Claudin-1, Occludin, E-cadherin). Furthermore, it modulated apoptosis-related proteins (reduced Bax, Caspase-3; increased Bcl-2) promoted epithelial migration.

Язык: Английский

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