Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Апрель 17, 2025

Culture platforms that closely mimic the spatial architecture, cellular diversity, and extracellular matrix composition of native tissues can serve as invaluable tools for a range scientific discovery biomedical applications. Organoids have emerged promising alternative to both traditional 2D cell culture animal models, offering physiologically relevant 3D system studying human biology. provide manipulable platform investigate organ development function well model patient-specific phenotypes. This mini review examines various methods used culturing organoids normal disease conditions in gastrointestinal tissues. We focus on how media formulations impact signaling, altering baseline phenotypes response perturbations. discuss future directions optimizing organoid improve basic translational potential.

Язык: Английский