Functional characterization of an epsilon glutathione S-transferase (SfGSTe9) associated with insecticide detoxification in Spodoptera frugiperda

Pesticide Biochemistry and Physiology, Год журнала: 2025, Номер 208, С. 106305 - 106305

Опубликована: Янв. 21, 2025

Язык: Английский

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Insect Resistance to Insecticides: Causes, Mechanisms, and Exploring Potential Solutions

Archives of Insect Biochemistry and Physiology, Год журнала: 2025, Номер 118(2)

Опубликована: Фев. 1, 2025

ABSTRACT Insecticides play a crucial role as the primary means of controlling agricultural pests, preventing significant damage to crops. However, misuse these insecticides has led development resistance in insect pests against major classes chemicals. The emergence poses serious threat, especially when alternative options for crop protection are limited farmers. Addressing this challenge and developing new, effective, sustainable pest management approaches is not merely essential but also critically important. In absence solutions, understanding root causes behind insects becomes critical necessity. Without understanding, formulation effective combat remains elusive. With playing vital global food security public health, mitigating paramount. Given growing concern over insecticides, review addresses research gap by thoroughly examining causes, mechanisms, potential solutions. examines factors driving resistance, such evolutionary pressure excessive pesticide use, provides detailed analysis including detoxifying enzyme overproduction target site mutations. Providing an it discusses integrated management, strategic insecticide rotation, use new control technologies biological agents. Emphasizing urgency multifaceted approach, concise roadmap guiding future applications.

Язык: Английский

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Nicotinamide deficiency promotes imidacloprid resistance via activation of ROS/CncC signaling pathway-mediated UGT detoxification in Nilaparvata lugens

The Science of The Total Environment, Год журнала: 2024, Номер 926, С. 172035 - 172035

Опубликована: Март 31, 2024

Язык: Английский

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Emerging technological developments to address pest resistance in Bt cotton

Journal of Cotton Research, Год журнала: 2024, Номер 7(1)

Опубликована: Сен. 3, 2024

Abstract Cotton plays a crucial role in shaping Indian economy and rural livelihoods. The cotton crop is prone to numerous insect pests, necessitating insecticidal application, which increases production costs. advent of the expression Bacillus thuringiensis (Bt) protein has significantly reduced burden pest without compromising environmental or human health. After introduction transgenic cotton, cultivated area expanded 22 million hectares, with 64% increase adoption by farmers worldwide. Currently, Bt accounts for 93% India. However, extensive use accelerated resistance development pests like pink bollworm. Furthermore, overreliance on broad-spectrum pesticides, favouring emergence secondary significant challenges. This emphasizes urgent necessity developing novel management strategies. high-dose refuge strategy was initially effective managing but its implementation India faced challenges due misunderstandings about non-Bt crops. Although gene pyramiding introduced as solution, combining mono toxin also led instances cross-resistance. Therefore, there need further exploration biotechnological approaches manage cotton. Advanced strategies, such sterile release, RNA interference (RNAi)-mediated silencing, stacking RNAi, genome editing using clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR-Cas), offer promising tools identifying genes insects. Additionally, CRISPR-mediated drives biopesticides present potential avenues cultivation. These innovative could enhance sustainability efficacy

Язык: Английский

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Intercropping-induced leaf metabolic changes increase plant resistance to herbivory

Plant and Soil, Год журнала: 2024, Номер unknown

Опубликована: Янв. 11, 2024

Язык: Английский

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Tetrahymena thermophila glutathione-S-transferase superfamily: an eco-paralogs gene network differentially responding to various environmental abiotic stressors and an update on this gene family in ciliates

Frontiers in Genetics, Год журнала: 2025, Номер 16

Опубликована: Март 7, 2025

Glutathione S-transferases constitute a superfamily of enzymes involved mainly, but not exclusively, in the detoxification xenobiotic compounds that are considered environmental pollutants. In this work, an updated analysis putative cytosolic glutathione (cGST) from ciliate protozoa is performed although mainly focused on Tetrahymena thermophila . Among ciliates, genus has highest number (58 average) cGST genes. As mammals, Mu class present all analyzed ciliates and majority species. After occurrence GST domains T. , out 54 GSTs previously to be class, six them have been discarded as they do recognizable domains. addition, there one species-specific another GST-EF1G (elongation factor 1 gamma). A structural shown wide variety β-sheets/α-helix patterns, most abundant being canonical thioredoxin-folding pattern. Within categories bZIP C4 zinc finger transcription factors, potential binding sites for c-Jun c-Fos (32% average), along with GATA-1 (71% gene promoters. The alignment MAPEG (Membrane Associated Proteins Eicosanoid metabolism) protein sequences species shows family divided into two well-defined clans. phylogenetic cluster 19 Mu-class genes predecessors members omega, theta zeta classes. This means current model needs modified. Sixteen genes, together clusters including three each very high identity, selected qRT-PCR under stress eleven different stressors. revealed respond selectively and/or differentially stressor, independently which it belongs. Most more toxic metal(loid)s used (Cd or As).

Язык: Английский

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GSTD1 Mediates the Tolerance to Abamectin and Beta-Cypermethrin in the Fall Armyworm Spodoptera frugiperda

Insects, Год журнала: 2025, Номер 16(3), С. 299 - 299

Опубликована: Март 12, 2025

Glutathione S-transferase (GST) is a class of detoxifying enzymes in the second stage insect metabolism and plays key role insecticide resistance. In this study, based on transcriptome sequences S. frugiperda, full-length cDNA SfGSTD1 was cloned characterized. The temporal spatial expression pattern showed that highly expressed Malpighian tubules, which are excretion organs. Knocking down reduced frugiperd tolerance to abamectin beta-cypermethrin. overexpression enhanced viability Sf9 cell under beta-cypermethrin treatment. Furthermore, overexpressed Drosophila melanogaster using GAL4/UAS binary system, strain also less susceptible enzyme activity recombinant could be significantly inhibited by Taken together, our findings indicate might involved frugiperda. And these results provide theoretical foundations for understanding resistance mechanism frugiperda

Язык: Английский

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Establishing a fluorescence-based technique for ABC transporters functional analysis in metabolism of insecticides in a Lepidopteron

Environmental Technology & Innovation, Год журнала: 2024, Номер 35, С. 103719 - 103719

Опубликована: Июнь 15, 2024

The insecticide resistance development poses challenges to the pest control effectiveness. As phase III detoxification enzyme, ATP-binding cassette (ABC) transporters play a crucial role in conferring by facilitating elimination of insecticides. However, precise involvement ABCs insecticides metabolism remains unclear. In this study, competitive assay utilizing microinjection ABC substrate Texas Red (TR) was established Lepidoptera for first time investigate contribution process Cydia pomonella towards lambda-cyhalothrin (LCT) and abamectin (ABM). Results revealed that TR hindered inhibitor verapamil, LCT, or combination both, implying shared subsets involved transportation LCT. Moreover, application ABM alone led marked inhibition elimination, with verapamil not further exacerbating inhibitory effect, indicating exhibited highly characteristic has strong interaction them. Furthermore, silencing key associated genes through RNAi resulted increased mortality larvae under LCT exposure, affecting ATP hydrolysis. These findings imply are critical active transport function energized ATP, ultimately reducing tolerance conclusion, our only validated feasibility bioassay using fluorescent lepidopteran but also highlighted enhancing efflux capacity C. larvae, binding their metabolites.

Язык: Английский

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Elucidating the detoxification efficacy of Periplaneta americana delta glutathione S-transferase 1 (PaGSTd1) against organophosphates

Pesticide Biochemistry and Physiology, Год журнала: 2024, Номер 203, С. 106013 - 106013

Опубликована: Июль 4, 2024

Язык: Английский

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How to Identify Pesticide Targets?

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Pesticides are essential in contemporary agriculture, as they improve crop yields and quality while safeguarding against pests. However, long-term heavy use of traditional pesticides has led to increased pest resistance, these often toxic less selective, may also have adverse effects on the environment nontarget organisms. To solve this problem, it is important find new targets for pesticide develop more effective environmentally friendly alternatives. Therefore, exploring action become one current research focuses. In past years, efforts been made investigate possible strategies, work systematically summarizes criticizes most recently used ones. This contribution not only helps promote development but holds substantial implications promoting sustainable agriculture food security.

Язык: Английский

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