npj Biofilms and Microbiomes,
Год журнала:
2024,
Номер
10(1)
Опубликована: Ноя. 13, 2024
Microbiome
restoration
using
beneficial
microorganisms
for
corals
(BMCs)
comprise
a
promising
strategy
to
help
cope
with
anthropogenic
stressors.
However,
there
is
limited
knowledge
on
the
uptake
of
BMCs
by
nontarget
animals,
especially
sponges.
This
study
explores
whether
sponges
can
acquire
upon
direct
application
and
inoculations
affect
sponge
health.
A
4-week
field
experiment
applying
Stylissa
carteri
Callyspongia
crassa
assessed
three
conditions:
no
inoculation,
inoculation
once
thrice
week.
BMC-related
strains
were
naturally
present
in
seawater
microbiome
S.
carteri.
These
enriched
response
only
microbiome.
Microbiomes
both
restructured;
visually
healthy
efficiently
pumped
water
at
end
experiment.
results
suggest
that
be
strains,
BMC
nearby
unlikely
negatively
Environmental Microbiology,
Год журнала:
2025,
Номер
27(2)
Опубликована: Янв. 31, 2025
Corals
associate
with
a
diverse
community
of
prokaryotic
symbionts
that
provide
nutrition,
antioxidants
and
other
protective
compounds
to
their
host.
However,
the
influence
microbes
on
coral
thermotolerance
remains
understudied.
Here,
we
examined
microbial
communities
associated
colonies
Acropora
cf.
tenuis
exhibit
high
or
low
upon
exposure
33°C
(heated)
relative
29°C
(control).
Using
16S
rRNA
sequencing,
show
structure
all
A.
was
similar
each
at
control
temperature.
Thermotolerant
colonies,
however,
had
relatively
greater
abundance
Endozoicomonas,
Arcobacter,
Bifidobacterium
Lactobacillus.
At
elevated
temperature,
only
thermosensitive
showed
distinct
shift
in
microbiome,
an
increase
Flavobacteriales,
Rhodobacteraceae
Vibrio,
accompanying
marked
bleaching
response.
Functional
prediction
indicated
thermotolerant
corals
were
enriched
for
genes
related
metabolism,
while
microbiomes
cell
motility
antibiotic
compound
synthesis.
These
differences
may
contribute
variable
performance
under
thermal
stress.
Identification
taxa
correlated
provides
insights
into
beneficial
bacterial
groups
could
be
used
microbiome
engineering
support
reef
health
changing
climate.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Июнь 24, 2024
Abstract
The
application
of
beneficial
microorganisms
for
corals
(BMC)
decreases
the
bleaching
susceptibility
and
mortality
rate
corals.
BMC
selection
is
typically
performed
via
molecular
biochemical
assays,
followed
by
genomic
screening
traits.
Herein,
we
present
a
comprehensive
in
silico
framework
to
explore
set
six
putative
strains.
We
extracted
high-quality
DNA
from
coral
samples
collected
Red
Sea
PacBio
sequencing.
identified
traits
mechanisms
associated
with
each
strain
as
well
proposed
new
mechanisms,
such
chemotaxis
presence
phages
bioactive
secondary
metabolites.
prophages
two
studied
strains
suggests
their
possible
distribution
within
bacteria.
also
detected
various
metabolites,
terpenes,
ectoines,
lanthipeptides,
lasso
peptides.
These
metabolites
possess
antimicrobial,
antifungal,
antiviral,
anti-inflammatory,
antioxidant
activities
play
key
roles
health
reducing
effects
heat
stress,
high
salinity,
reactive
oxygen
species,
radiation.
Corals
are
currently
facing
unprecedented
challenges,
our
revised
can
help
select
more
efficient
use
studies
on
microbiome
rehabilitation,
resilience,
restoration.
New Phytologist,
Год журнала:
2024,
Номер
244(2), С. 364 - 376
Опубликована: Авг. 13, 2024
In
the
marine
environment,
seaweeds
(i.e.
macroalgae)
provide
a
wide
range
of
ecological
services
and
economic
benefits.
Like
land
plants,
do
not
these
in
isolation,
rather
they
rely
on
their
associated
microbial
communities,
which
together
with
host
form
seaweed
holobiont.
However,
there
is
poor
understanding
mechanisms
shaping
complex
seaweed-microbe
interactions,
evolutionary
processes
underlying
interactions.
Here,
we
identify
current
research
challenges
opportunities
field
holobiont
biology.
We
argue
that
identifying
key
partners,
knowing
how
are
recruited,
specific
function
relevance
across
all
life
history
stages
among
knowledge
gaps
particularly
important
to
address,
especially
context
environmental
threatening
seaweeds.
further
discuss
future
approaches
study
holobionts,
can
apply
concept
natural
or
engineered
ecosystems.
Coral
reefs
are
hotspots
of
marine
biodiversity,
which
results
in
the
synthesis
a
wide
variety
compounds
with
unique
molecular
scaffolds,
and
bioactivities,
rendering
an
ecosystem
interest.
The
chemodiversity
stems
from
intricate
relationships
between
inhabitants
reef,
as
chemistry
produced
partakes
intra-
interspecies
communication,
settlement,
nutrient
acquisition,
defense.
However,
coral
declining
at
unprecedented
rate
due
to
climate
change,
pollution,
increased
incidence
pathogenic
diseases.
Among
pathogens,
Vibrio
spp.
bacteria
key
players
resulting
high
mortality.
Thus,
alternative
strategies
such
application
beneficial
isolated
disease-resilient
species
being
explored
lower
burden
species.
Here,
we
apply
coculturing
coral-derived
leverage
recent
advancements
untargeted
metabolomics
discover
engineerable
traits.
By
chasing
chemical
change
coculture,
report
Microbulbifer
spp.-mediated
degradation
amphibactins,
by
sequester
iron.
Additional
biochemical
experiments
revealed
that
occurs
peptide
backbone
requires
enzyme
fraction
Microbulbifer.
A
reduction
iron
affinity
is
expected
loss
one
Fe(III)
binding
moiety.
Therefore,
hypothesize
this
shapes
community
behaviors
it
pertains
limiting
environment,
survival.
Furthermore,
sp.
suppressed
natural
product
bacteria.
Understanding
mechanisms
behind
these
interactions
will
enable
engineering
probiotic
capable
lowering
burdens
during
heat
waves
disease.
