Unique Immune and Other Responses of Human Nasal Epithelial Cells Infected with H5N1 Avian Influenza Virus Compared to Seasonal Human Influenza A and B Viruses DOI Creative Commons

Tan Kai Sen,

Jing Liu, A. Alwin Prem Anand

и другие.

Emerging Microbes & Infections, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Highly pathogenic avian influenza (HPAI) virus (e.g. H5N1) infects the lower airway to cause severe infections, and constitute a prime candidate for emergence of disease X. The nasal epithelium is primary portal entry respiratory pathogens, serving as airway's physical immune barrier. While HPAI predominantly airway, not much known about its interactions with epithelium. Hence, we sought elucidate compare differential responses against infection that may contribute pathology, identify critical response markers. We infected human epithelial cells (hNECs) cultured at air-liquid interface from multiple healthy donors clinical isolates major seasonal viruses (H1N1, H3N2, B) H5N1. were subjected virologic, transcriptomic secretory protein analyses. less adapted infecting epithelium, H5N1 elicited unique host unlike influenza. Interestingly, hNECs induced indicative subdued antiviral activity reduced expression IFNβ, inflammasome mediators, IL-1α IL-1β); decreased wound healing; suppressed re-epithelialization; compromised barrier integrity; diminished oxidative stress; increased transmembrane solute ion carrier gene expression. These molecular changes in represent potential targets enhancing diagnostic therapeutic strategies better surveillance management humans.

Язык: Английский

Understanding the regulation of chronic wounds by tissue inhibitors of matrix metalloproteinases through mathematical modelling DOI Creative Commons
Sonia Dari, Reuben D. O’Dea, Nabil T. Fadai

и другие.

Journal of Theoretical Biology, Год журнала: 2025, Номер unknown, С. 112083 - 112083

Опубликована: Фев. 1, 2025

Understanding the biochemistry and pharmacodynamics of chronic wounds is key importance, due to millions people in UK affected significant cost NHS. Chronic are characterised by elevated concentrations matrix metalloproteinases (MMPs) that destroy surrounding extracellular (ECM). However, fibroblasts can produce tissue inhibitors MMPs (TIMPs) order regulate wound healing. Therefore, role TIMPs both acute needs be properly understood develop therapeutic treatments. In this work, we propose a reaction-diffusion system four partial differential equations describe interaction ECM, fibroblasts, MMPs, wound. We observe that, subject parameter sets corresponding healing, mathematical model gives rise travelling wave solutions. Using bifurcation analysis, demonstrate excessive degradation ECM results emergence wounds, reversal these prohibited for lower TIMP production values. These replicated within simplified obtained via sensitivity analysis. This further extended more realistic spatial domains where effectiveness hydrogel containing as treatment wounds.

Язык: Английский

Процитировано

0
Strain‐Dependent Differences in Inflammatory/Immune Activity in Cutaneous Wound Tissue Repair in Rats: The Significance of Body Mass/Proneness to Obesity DOI Creative Commons
Jelena Kulaš, Aleksandra Popov Aleksandrov, Dušanka Popović

и другие.

Mediators of Inflammation, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Inflammatory/immune cells and mediators are substantial for wound healing because they orchestrate biological activities in this complex process. Among factors that affect healing, obesity, metabolic diseases among the most significant, particularly of a relationship between obesity prediabetic state with immune reactivity. Using Dark Agouti (DA) Albino Oxford (AO) rats, which differ responses as well proneness to we examined impact these intrinsic on cutaneous healing. Dynamics process were monitored at days 3, 5, 7 post-wounding parallel both rat strains by analysis selected basic aspects repair (cytokine growth factor responses) granulation tissue. Strain-related differences extent reduction area shown, coincided differential proinflammatory immune-regulatory cytokines, response rats. Some seem related their dissimilarities obesity. Results study extended so far known inflammatory/immune variety stimuli AO DA rats showed, first time, immune-based body mass (BM) (under ad libitum feeding conditions normal rodent chow).

Язык: Английский

Процитировано

0
Cold Atmospheric Plasma as a Promising Medical Device for Wound Healing: Implications from Protein Perspective DOI
Priya Bhatt,

Reema Reema,

Kamatchi Sankaranarayanan

и другие.

Regenerative Engineering and Translational Medicine, Год журнала: 2025, Номер unknown

Опубликована: Апрель 11, 2025

Язык: Английский

Процитировано

0
The Modulatory Effect of Selol (Se IV) on Pro-Inflammatory Pathways in RAW 264.7 Macrophages DOI Open Access
Gwan Yong Lim, Emilia Grosicka-Maciąg,

Maria Szumiło

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 559 - 559

Опубликована: Янв. 10, 2025

Selol is a semi-synthetic mixture of selenized triglycerides. The results biological studies revealed that exhibits several anticancer effects. However, on its potential anti-inflammatory activity are scarce, and underlying signaling pathways unknown. aim our study was to investigate the ability exert effects in RAW 264.7 cell line model LPS (lipopolysaccharide)-induced inflammation. Cells were treated either with 5% (4 or 8 µg Se/mL) (1 µg/mL) alone given concomitantly LPS. parameters studied reactive oxygen species (ROS) production, glutathione thioredoxin (Txn) levels, nuclear factor kappa B (NF-κB) activation, as well nitric oxide/prostaglandin E2 (NO/PGE2) production. presented research also included effect and/or glucose (Glc) catabolism; for this purpose, levels key enzymes glycolysis pathway determined. showed exhibited pro-oxidative properties. It induced ROS generation significant increase level Txn; however, it did not affect reduced glutathione/oxidized (GSH/GSSG) ratio. moderately activated NF-κB but failed NO/PGE2 catabolism significant. simultaneous administration exerted statistically via decrease production pro-inflammatory mediators activation. Our result action cells, anaerobic increased, incubation caused partial reprogramming Glc metabolism towards aerobic metabolism. This may indicate different pharmacological molecular physiological pathological conditions.

Язык: Английский

Процитировано

0
Development of Electrospun Nanofibrous Composite Membrane of Tannic Acid–Cellulose Acetate: Advancing Antimicrobial and Free Radical Scavenging Activity DOI Creative Commons
Hanlin Chen, Liying Lin,

Uyen Khanh Pham

и другие.

Carbohydrate Polymer Technologies and Applications, Год журнала: 2025, Номер 9, С. 100666 - 100666

Опубликована: Янв. 10, 2025

Язык: Английский

Процитировано

0
Chlorogenic acid-assisted dopamine‑sodium alginate composite nanofiber membranes for promoting wound healing DOI
Meng Zhang, Yang Wang,

Xueling Yin

и другие.

Carbohydrate Polymers, Год журнала: 2025, Номер 354, С. 123298 - 123298

Опубликована: Янв. 23, 2025

Язык: Английский

Процитировано

0
Inverse Oxide/Alloy‐Structured Nanozymes with NIR‐Triggered Enzymatic Cascade Regulation of ROS Homeostasis for Efficient Wound Healing DOI Open Access

Yongsen Zhao,

Shiqi Zhao, Yu Du

и другие.

Advanced Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 25, 2025

The precise spatiotemporal control of reactive oxygen species (ROS) generation and scavenging remains pivotal for infected wound healing. However, conventional nanozymes fail to adaptively regulate ROS dynamics across inflammatory proliferative phases. A near-infrared (NIR)-activated inverse oxide/alloy-structured nanozyme (Co7Fe3/ZnO@C) is developed, featuring enzymatic cascade activities tune homeostasis through synergistic chemodynamic (CDT), photodynamic (PDT), photothermal (PTT) therapies. orchestrates a self-regulated cascade: peroxidase (POD)-like activity initially generates bactericidal hydroxyl radicals in acidic wounds, while subsequent NIR triggers hot electron transfer from Co7Fe3 ZnO, facilitating synchronized superoxide dismutase (SOD)-like, catalase (CAT)-like radical antioxidant capacity (HORAC) scavenge residual ROS. This cascaded network dynamically balances production (POD) (NIR-driven SOD/CAT/HORAC), eradicating bacteria resolving inflammation. In vitro/vivo studies have shown that the proposed method maintaining can markedly enhance rate healing by regulation environment within injured tissue facilitation rapid re-epithelialization. work provides an intelligent platform simulates function natural enzymes constructs reaction strategy balance antibacterial anti-inflammatory demands microenvironment.

Язык: Английский

Процитировано

0
Microemulsions Improve the Cutaneous Co-Localization of Lipoic Acid and Quercetin and Antioxidant Effects in Cutaneous Cells and Tissue DOI

Maria Isabella da Silva,

Julia S. Passos, Regina G. Daré

и другие.

AAPS PharmSciTech, Год журнала: 2025, Номер 26(3)

Опубликована: Фев. 26, 2025

Язык: Английский

Процитировано

0
Death fuels growth: Emerging players bridging apoptosis and cell proliferation in Drosophila and beyond DOI Creative Commons

Caitlin Hounsell,

Yun Fan

Seminars in Cell and Developmental Biology, Год журнала: 2025, Номер 169, С. 103602 - 103602

Опубликована: Март 12, 2025

Tissue homeostasis relies on a delicate balance between cell death and proliferation. Apoptosis plays key role not only in removing damaged cells but also promoting tissue recovery through process known as apoptosis-induced proliferation (AiP). This review highlights how caspases, c-Jun N-terminal Kinase (JNK), Reactive Oxygen Species (ROS) bridge proliferation, revealed studies using Drosophila model organism. We compare these findings with advances other systems discuss their broader implications for regeneration tumorigenesis.

Язык: Английский

Процитировано

0
Bioactive hemostatic materials: a new strategy for promoting wound healing and tissue regeneration DOI Creative Commons
Zhengyuan Liu,

Junnan Xu,

Xing Wang

и другие.

MedComm, Год журнала: 2025, Номер 6(4)

Опубликована: Март 22, 2025

Abstract Wound healing remains a critical global healthcare challenge, with an annual treatment cost exceeding $50 billion worldwide. Over the past decade, significant advances in wound care have focused on developing sophisticated biomaterials that promote tissue regeneration and prevent complications. Despite these developments, there crucial need for multifunctional materials can effectively address complex, multiphase nature of repair while being effective easily applicable various clinical settings. This review systematically analyzes latest developments materials, examining their chemical composition, structural design, therapeutic mechanisms. We comprehensively evaluate bioactive components, including natural polymers, synthetic matrices, hybrid composites, along different forms, such as hydrogels, powders, smart dressings. Special attention is given to emerging strategies material design integrate multiple functions, sustained drug delivery, infection prevention, promotion. The insights provided this illuminate path toward next‐generation highlighting opportunities more solutions significantly improve patient outcomes reduce burden.

Язык: Английский

Процитировано

0