Revealing microRNA regulation in single cells DOI Creative Commons
Ranjan Kumar Maji, Matthias S. Leisegang, Reinier A. Boon

и другие.

Trends in Genetics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

MicroRNAs (miRNAs) are key regulators of gene expression and control cellular functions in physiological pathophysiological states. miRNAs play important roles disease, stress, development, now being investigated for therapeutic approaches. Alternative processing during biogenesis results the generation miRNA isoforms (isomiRs) which further diversify regulation. Single-cell RNA-sequencing (scsRNA-seq) technologies, together with computational strategies, enable exploration miRNAs, isomiRs, interacting RNAs at level. By integration other miRNA-associated single-cell modalities, can be resolved different stages In this review we discuss (i) experimental assays that measure isomiR abundances, (ii) methods their analysis to investigate mechanisms post-transcriptional

Язык: Английский

Revealing microRNA regulation in single cells DOI Creative Commons
Ranjan Kumar Maji, Matthias S. Leisegang, Reinier A. Boon

и другие.

Trends in Genetics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

MicroRNAs (miRNAs) are key regulators of gene expression and control cellular functions in physiological pathophysiological states. miRNAs play important roles disease, stress, development, now being investigated for therapeutic approaches. Alternative processing during biogenesis results the generation miRNA isoforms (isomiRs) which further diversify regulation. Single-cell RNA-sequencing (scsRNA-seq) technologies, together with computational strategies, enable exploration miRNAs, isomiRs, interacting RNAs at level. By integration other miRNA-associated single-cell modalities, can be resolved different stages In this review we discuss (i) experimental assays that measure isomiR abundances, (ii) methods their analysis to investigate mechanisms post-transcriptional

Язык: Английский

Процитировано

2