Spontaneous Hollow Coacervate Transition of Silk Fibroin via Dilution and Its Transition to Microcapsules
Biomacromolecules,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 10, 2025
Polymeric
microcapsules
are
useful
for
drug
delivery,
microreactors,
and
cargo
transport,
but
traditional
fabrication
methods
require
complex
processes
harsh
conditions.
Coacervates,
formed
by
liquid–liquid
phase
separation
(LLPS),
offer
a
promising
alternative
microcapsule
fabrication.
Recent
studies
have
shown
that
coacervates
can
spontaneously
form
hollow
cavities
under
specific
Here,
we
investigate
the
spontaneous
coacervate
transition
of
silk
fibroin
(SF).
SF
coacervates,
induced
mixing
with
dextran,
calcium
ions,
copper
to
upon
dilution.
Adding
ethylenediaminetetraacetic
acid
(EDTA)
further
transforms
them
into
vesicle-like
capsule
which
solidify
microcapsules.
As
proof-of-concept,
successfully
loaded
high-molecular-weight
polymer
cavity
bioactive
enzyme
layer
simply
solution.
Our
results
demonstrate
facile,
organic-solvent-free
approach
fabricating
SF-based
provide
insight
mechanisms
driving
formation.
Язык: Английский
The mechanobiology of biomolecular condensates
Biophysics Reviews,
Год журнала:
2025,
Номер
6(1)
Опубликована: Март 1, 2025
The
central
goal
of
mechanobiology
is
to
understand
how
the
mechanical
forces
and
material
properties
organelles,
cells,
tissues
influence
biological
processes
functions.
Since
first
description
biomolecular
condensates,
it
was
hypothesized
that
they
obtain
are
tuned
their
functions
inside
cells.
Thus,
represent
an
intriguing
playground
for
mechanobiology.
idea
condensates
exhibit
diverse
adaptive
highlights
need
different
states
respond
external
whether
these
responses
linked
physiological
roles
within
cell.
For
example,
liquids
buffer
dissipate,
while
solids
store
transmit
stress,
relaxation
time
a
viscoelastic
can
act
as
frequency
filter.
Hence,
liquid-solid
transition
condensate
in
force
transmission
pathway
determine
signals
transduced
in-between
affecting
differentiation,
neuronal
network
dynamics,
behavior
stimuli.
Here,
we
review
our
current
understanding
molecular
drivers
rigidity
phase
transitions
set
forth
complex
cellular
environment.
We
will
then
summarize
technical
advancements
were
necessary
insights
into
rich
fascinating
finally,
highlight
recent
examples
connection
specific
Our
provide
comprehensive
summary
field
on
cells
harness
regulate
mechanics
achieve
Язык: Английский
Sustainable Spinning of Artificial Spider Silk Fibers with Excellent Toughness and Inherent Potential for Functionalization
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 26, 2024
Abstract
Despite
impressive
progress
in
the
field,
there
are
still
several
major
bottlenecks
producing
fibers
from
recombinantly
produced
spider‐silk‐like
proteins
to
replicate
extraordinary
mechanical
properties
of
spider
ampullate
silk.
The
conventional
artificial
fiber
spinning
processes
rely
primarily
on
organic
solvents
coagulate
into
and
require
complex
post‐treatments
obtain
with
valuable
properties.
This
is
due
challenges
obtaining
soluble
silk
proteins,
but
also
because
native
process
leading
hierarchical
organization
not
fully
understood
hard
a
manner
applicable
industrial
settings.
Here,
recombinant
spider‐silk
fusion
efficiently
processed
as‐spun
toughness
modulus
120
MJ
m
−3
extensibility
255%
using
solely
aqueous
solutions.
assemble
similar
that
reported
for
silk:
they
phase
separate
induced
by
salting
out,
followed
alignment
secondary
structure
transition
triggered
shear
forces
dehydration.
Finally,
design
enables
straightforward
functionalization
under
mild
all‐aqueous
conditions
via
simple
biomolecular
click
reaction
both
pre‐
post‐spinning.
Язык: Английский
Recombinant silk protein condensates show widely different properties depending on sample background
Journal of Materials Chemistry B,
Год журнала:
2024,
Номер
12(46), С. 11953 - 11967
Опубликована: Янв. 1, 2024
Background
molecules
such
as
DNA
affect
the
formation
and
properties
of
recombinant
spider
silk
protein
condensates.
Язык: Английский