Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 4, 2025
Abstract
Ovary
is
a
vital
and
dynamic
reproductive
organ.
Ovarian
tissue
cryopreservation
(OTC)
plays
role
in
female
fertility
preservation,
especially
for
adolescent
cancer
patients.
Yet,
the
sensitive
cell
populations
cryoinjury
molecular
mechanisms
underlying
remain
elusive.
This
study
employs
single-cell
RNA
sequencing
spatial
transcriptomics
to
dissect
impacts
of
temperature
stress
cryoprotectant
toxicity
on
human
ovarian
cortex.
The
spatiotemporal
characteristics
eight
types
following
vitrification-thawing
had
been
comprehensively
characterized.
Granulosa,
perivascular
stromal
cells
are
identified
as
most
OTC
procedure.
Further
analysis
using
Smart-seq2
oocytes
highlights
alterations
in“cell
cycle”
“DNA
methylation”
pathways.
Notably,
FOS/AP-1
pathway
emerges
crucial
response
factor
during
cryopreservation.
Inhibition
this
with
T-5224
mitigates
vascular
damage
reduces
apoptosis
vitrification-thawed
ovaries.
These
findings
provide
insight
into
dynamics
vitrification
thawing,
aiding
prioritizing
therapeutic
strategies
pre-
post-cryopreservation
interventions.
The
ovary
is
a
crucial
gonadal
organ
that
supports
female
reproductive
and
endocrine
functions.
Ovarian
aging
can
result
in
decreased
fertility
dysfunction
across
multiple
organs.
Research
has
demonstrated
cellular
senescence
various
cell
types
within
the
trigger
decline
ovarian
function
through
distinct
stress
responses,
resulting
aging.
This
review
explores
how
may
contribute
to
failure.
Additionally,
we
discuss
factors
cause
senescence,
including
accumulation
of
advanced
glycation
end
products,
oxidative
stress,
mitochondrial
dysfunction,
DNA
damage,
telomere
shortening,
exposure
chemotherapy.
Furthermore,
six
types,
oocytes,
granulosa
cells,
theca
immune
surface
epithelium,
endothelial
inside
explore
their
contribution
accelerated
Lastly,
describe
potential
senotherapeutics
for
treatment
offer
novel
strategies
longevity.
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 3, 2025
Cellular
aging
is
a
multifactorial
and
intricately
regulated
physiological
process
with
profound
implications.
The
interaction
between
cellular
senescence
cancer
complex
multifaceted,
can
both
promote
inhibit
tumor
progression
through
various
mechanisms.
M6A
methylation
modification
regulates
the
of
cells
tissues
by
modulating
senescence-related
genes.
In
this
review,
we
comprehensively
discuss
characteristics
senescence,
signaling
pathways
regulating
biomarkers
mechanisms
anti-senescence
drugs.
Notably,
review
also
delves
into
interactions
cancer,
emphasizing
dual
role
senescent
microenvironment
in
initiation,
progression,
treatment.
Finally,
thoroughly
explore
function
mechanism
m6A
revealing
its
critical
gene
expression
maintaining
homeostasis.
conclusion,
provides
comprehensive
perspective
on
molecular
biological
significance
offers
new
insights
for
development
strategies.
Human Reproduction Update,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 20, 2025
Ovarian
aging
occurs
earlier
than
the
of
many
other
organs
and
has
a
lasting
impact
on
women's
overall
health
well-being.
However,
effective
interventions
to
slow
ovarian
remain
limited,
primarily
due
an
incomplete
understanding
underlying
molecular
mechanisms
drug
targets.
Recent
advances
in
omics
data
resources,
combined
with
innovative
computational
tools,
are
offering
deeper
insight
into
complexities
aging,
paving
way
for
new
opportunities
discovery
development.
This
review
aims
synthesize
expanding
multi-omics
data,
spanning
genome,
transcriptome,
proteome,
metabolome,
microbiome,
related
from
both
tissue-level
single-cell
perspectives.
We
will
specially
explore
how
analysis
these
emerging
datasets
can
be
leveraged
identify
novel
targets
guide
therapeutic
strategies
slowing
reversing
aging.
conducted
comprehensive
literature
search
PubMed
database
using
range
relevant
keywords:
age
at
natural
menopause,
premature
insufficiency
(POI),
diminished
reserve
(DOR),
genomics,
transcriptomics,
epigenomics,
DNA
methylation,
RNA
modification,
histone
proteomics,
metabolomics,
lipidomics,
single-cell,
genome-wide
association
studies
(GWAS),
whole-exome
sequencing,
phenome-wide
(PheWAS),
Mendelian
randomization
(MR),
epigenetic
target,
machine
learning,
artificial
intelligence
(AI),
deep
multi-omics.
The
was
restricted
English-language
articles
published
up
September
2024.
Multi-omics
have
uncovered
key
driving
including
damage
repair
deficiencies,
inflammatory
immune
responses,
mitochondrial
dysfunction,
cell
death.
By
integrating
researchers
critical
regulatory
factors
across
various
biological
levels,
leading
potential
Notable
examples
include
genetic
such
as
BRCA2
TERT,
like
Tet
FTO,
metabolic
sirtuins
CD38+,
protein
BIN2
PDGF-BB,
transcription
FOXP1.
advent
cutting-edge
technologies,
especially
technologies
spatial
provided
valuable
insights
guiding
treatment
decisions
become
powerful
tool
aimed
mitigating
or
As
technology
advances,
integration
AI
models
holds
more
accurately
predict
candidate
convergence
offers
promising
avenues
personalized
medicine
precision
therapies,
tailored
Not
applicable.
Tendons
and
ligaments
are
crucial
connective
tissues
linking
bones
muscles,
yet
achieving
full
functional
recovery
after
injury
remains
challenging.
We
investigated
the
characteristics
of
tendon
stem/progenitor
cells
(TSPCs)
by
focusing
on
declining
repair
capacity
with
growth.
Using
single-cell
RNA
sequencing
Achilles
from
2-
6-week-old
mice,
we
identified
Cd55
Cd248
as
novel
surface
antigen
markers
for
TSPCs.
Combining
single-nucleus
ATAC
analyses
revealed
that
positive
fractions
in
tissue
TSPCs,
this
population
decreasing
at
1
weeks.
also
candidate
upstream
transcription
factors
regulating
these
fractions.
Functional
isolated
CD55/CD248
demonstrated
high
clonogenic
potential
differentiation
capacity,
forming
tendon-like
vitro
.
This
study
establishes
CD55
CD248
TSPC
antigens,
potentially
advancing
regenerative
medicine
contributing
to
development
new
treatment
strategies
ligament
injuries.
Tendons
and
ligaments
are
crucial
connective
tissues
linking
bones
muscles,
yet
achieving
full
functional
recovery
after
injury
remains
challenging.
We
investigated
the
characteristics
of
tendon
stem/progenitor
cells
(TSPCs)
by
focusing
on
declining
repair
capacity
with
growth.
Using
single-cell
RNA
sequencing
Achilles
from
2-
6-week-old
mice,
we
identified
Cd55
Cd248
as
novel
surface
antigen
markers
for
TSPCs.
Combining
single-nucleus
ATAC
analyses
revealed
that
positive
fractions
in
tissue
TSPCs,
this
population
decreasing
at
1
weeks.
also
candidate
upstream
transcription
factors
regulating
these
fractions.
Functional
isolated
CD55/CD248
demonstrated
high
clonogenic
potential
differentiation
capacity,
forming
tendon-like
vitro
.
This
study
establishes
CD55
CD248
TSPC
antigens,
potentially
advancing
regenerative
medicine
contributing
to
development
new
treatment
strategies
ligament
injuries.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 3, 2025
Abstract
Mouse
is
a
tractable
model
for
human
ovarian
biology,
however
its
utility
limited
by
incomplete
understanding
of
how
transcription
and
signaling
differ
interspecifically
with
age.
We
compared
ovaries
between
species
using
3D-imaging,
single-cell
transcriptomics,
functional
studies.
In
mice,
we
mapped
declining
follicle
numbers
oocyte
competence
during
aging;
in
ovaries,
identified
cortical
pockets
density
changes.
Oocytes
had
species-specific
gene
expression
patterns
growth
that
converged
toward
maturity.
Age-related
transcriptional
changes
were
greater
oocytes
than
granulosa
cells
across
species,
although
mature
change
more
humans.
sympathetic
nerves
glia;
nerve
increased
aged
and,
when
ablated
perturbed
folliculogenesis.
This
comparative
atlas
defines
shared
hallmarks
biology.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2471 - 2471
Опубликована: Март 10, 2025
Prenatal
stress
(PNS)
impairs
offspring
ovarian
development
by
exerting
negative
long-term
effects
on
postnatal
function
and
folliculogenesis.
FKBP51
is
a
stress-responsive
protein
that
inhibits
glucocorticoid
progesterone
receptors.
We
hypothesize
contributes
to
impaired
folliculogenesis
induced
PNS.
Timed-pregnant
Fkbp5+/+
(wild-type)
Fkbp5−/−
(knockout)
mice
were
randomly
assigned
either
the
undisturbed
(nonstress)
or
PNS
group,
with
exposure
maternal
restraint
from
embryonic
days
8
18.
Ovaries
harvested
stained,
follicles
counted
according
their
stages.
Ovarian
expressions
of
evaluated
immunohistochemistry
Fkbp5
steroidogenic
enzymes
qPCR.
Compared
controls,
had
increased
peripubertal
primordial
follicle
atresia
fewer
total
in
adult
middle-aged
groups.
In
offspring,
elevated
levels
granulosa
cells
primary
tertiary
follicles.
Our
results
suggest
administration
levels,
depleted
reserve,
dysregulated
steroid
synthesis.
However,
these
tolerated
mice,
supporting
conclusion
reduced
reserve
