Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 17, 2025
Abstract
Enzymatic
reductions
catalyzed
by
reductases,
such
as
aldo/ketoreductases
(AKRs),
imine
and
ene
reductases
generally
depend
on
the
regeneration
of
nicotinamide
cofactors
for
industrial
viability.
This
usually
involves
addition
a
cosubstrate
second
enzyme,
dehydrogenase,
e.g.
glucose
/
dehydrogenase.
For
commercial
viability
it
would
be
more
interesting
to
use
water
sacrificial
supply
necessary
hydrogen
atoms.
is
possible
using
photocatalytic
methods
involving
precious
metal,
Rh
Ru,
complexes
electron
mediators.
A
problem
associated
with
photobiocatalysis
in
general
weak
penetration
ultraviolet
or
visible
light
biological
systems.
Here
we
report
unprecedented
near
infrared
(NIR)
combination
hybrid
photoenzyme
based
IR
responsive
reductive
graphene
quantum
dots
(rGQDs)
that
are
non-toxic
AKRs.
We
envisaged
immobilization
AKR
close
proximity
rGQDs
could
enable
direct
transfer
atoms
from
prochiral
ketone
substrate
without
requiring
cofactor.
To
test
this
hypothesis,
immobilized
surface
cross-linked
AKR.
The
resulting
rGQD/AKR
photobiocatalyst
mediated
synthesis
pharmaceutical
intermediate,
(R)-1-[3,5-bis(trifluoromethyl)-phenyl]
ethanol
((R)-3,5-BTPE)
corresponding
ketone,
82%
yield
>
99.99%
ee
under
50
mW·cm−
2
illumination.
work
opens
new
avenues
create
artificial
photoenzymes
simplify
in
vitro
biocatalysis
coupling
renewable
solar
energy
sustainable
chemical
production.
Since
accounts
about
half
entire
spectrum
its
efficient
utilization
crucial
applications
sunlight.
have
demonstrated
our
can
effectively
utilize
catalyze
enzymatic
reduction
ketone.
these
photobiocatalysts
insoluble
solids
they
readily
recovered,
recycled
potentially
employed
continuous
flow
operation.
