Enabling neighbour labelling: using synthetic biology to explore how cells influence their neighbours
Development,
Год журнала:
2024,
Номер
151(1)
Опубликована: Янв. 1, 2024
ABSTRACT
Cell-cell
interactions
are
central
to
development,
but
exploring
how
a
change
in
any
given
cell
relates
changes
the
neighbour
of
that
can
be
technically
challenging.
Here,
we
review
recent
developments
synthetic
biology
and
image
analysis
helping
overcome
this
problem.
We
highlight
opportunities
presented
by
these
advances
discuss
limitations
applying
them
developmental
model
systems.
Язык: Английский
Live MSCs Characterizer Displays Stemness and Differentiation Using Colorful LV-cp Biosensors
ACS Sensors,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
Mesenchymal
stem
cells
(MSCs)
have
garnered
significant
attention
in
biomedical
research
due
to
their
accessibility
and
remarkable
differentiation
potential.
However,
the
lack
of
efficient
convenient
living
cell
monitoring
methods
limits
widespread
application
tissue
engineering
therapy.
Therefore,
we
present
progress
development
a
novel
series
fluorescent
protein
(FP)
sensors
based
on
turn-on
biosensors
(Turn-on
FPBs),
termed
LV-cp
biosensor
system
(novel
live
permuted
biosensors).
Utilizing
phage
display
technology
screen
for
affinity
peptides
specifically
targeting
MSCs
chondrocytes,
were
engineered
by
subcloning
these
into
proteins,
thereby
integrating
fluorescence
activation
mechanism
with
achieving
highly
accurate
detection
identification
two
types
using
as
"fluorescence
keys."
This
provides
simplified,
nontoxic
method
replace
traditional
antibody
kits,
strong
signals
can
be
obtained
through
various
devices.
In
addition,
enabled
dynamic
observation
chondrocytes
changes
colors.
Язык: Английский
Exploring stem cell frontiers: definitions, challenges, and perspectives for regenerative medicine
Biology Open,
Год журнала:
2024,
Номер
13(4)
Опубликована: Апрель 9, 2024
Each
year,
the
European
Summer
School
on
Stem
Cell
Biology
and
Regenerative
Medicine
(SCSS)
attracts
early-career
researchers
actively
practicing
clinicians
who
specialise
in
stem
cell
regenerative
biology.
The
16th
edition
of
this
influential
course
took
place
from
12th
to
19th
September
2023
charming
Greek
island
Spetses.
Focusing
important
concepts
recent
advances
cells,
distinguished
faculty
included
experts
spanning
spectrum
fundamental
research
clinical
trials
market-approved
therapies.
Alongside
an
academically
intensive
programme
that
bridges
various
contexts
research,
delegates
were
encouraged
critically
address
relevant
questions
biology
medicine,
including
broader
societal
implications.
Here,
we
present
a
comprehensive
overview
key
highlights
SCSS
2023.
Язык: Английский
An expanded view of cell competition
Development,
Год журнала:
2024,
Номер
151(22)
Опубликована: Ноя. 15, 2024
ABSTRACT
Cell
competition
arises
in
heterogeneous
tissues
when
neighbouring
cells
sense
their
relative
fitness
and
undergo
selection.
It
has
been
a
challenge
to
define
contexts
which
cell
is
physiologically
relevant
phenomenon
understand
the
cellular
features
that
underlie
sensing.
Drawing
on
examples
across
range
of
length
scales,
we
illuminate
molecular
could
diverse
tissue
types
processes
promote
reinforce
long-term
maintenance
function.
We
propose
by
broadening
scope
how
defined
circumstances
can
occur,
field
unlock
potential
as
lens
through
heterogeneity
its
role
fundamental
principles
complex
organisation
be
understood.
Язык: Английский