Frontiers in Cell and Developmental Biology,
Год журнала:
2020,
Номер
8
Опубликована: Окт. 6, 2020
Time-lapse
imaging
is
an
essential
tool
to
study
dynamic
biological
processes
that
cannot
be
discerned
from
fixed
samples
alone.
However,
cell-and
tissue-level
in
intact
animals
poses
numerous
challenges
if
the
organism
opaque
and/or
motile.
Explant
cultures
of
tissues
circumvent
some
these
challenges,
but
sample
drift
remains
a
considerable
obstacle.
We
employed
simple
yet
effective
technique
immobilize
medium-bathed
agarose.
applied
this
multiple
Drosophila
first-instar
larvae
adult
stages
various
orientations
and
with
no
evidence
anisotropic
pressure
or
stress
damage.
Using
method,
we
were
able
image
fine
features
for
up
18
hours
make
novel
observations.
Specifically,
report
fibers
characteristic
quiescent
neuroblasts
are
inherited
by
their
basal
daughters
during
reactivation;
lamina
developing
visual
system
assembled
roughly
2-3
columns
at
time;
glia
positions
development;
nuclear
envelopes
testis
cyst
stem
cells
do
not
break
down
completely
mitosis.
In
all,
demonstrate
our
protocol
wellsuited
tissue
immobilization
long-term
live
imaging,
enabling
new
insights
into
cell
dynamics
Drosophila.
Quantitative
analysis
of
the
dynamic
cellular
mechanisms
shaping
Drosophila
wing
during
its
larval
growth
phase
has
been
limited,
impeding
our
ability
to
understand
how
morphogen
patterns
regulate
tissue
shape.
Such
requires
imaging
explants
under
conditions
that
maintain
both
and
patterning,
as
well
methods
quantify
much
behaviors
change
Here,
we
demonstrate
a
key
requirement
for
steroid
hormone
20-hydroxyecdysone
(20E)
in
maintenance
numerous
patterning
systems
vivo
explant
culture.
We
find
low
concentrations
20E
support
prolonged
proliferation
explanted
discs
absence
insulin,
incidentally
providing
novel
insight
into
hormonal
regulation
imaginal
growth.
use
20E-containing
media
directly
observe
apply
recently
developed
quantitatively
decomposing
shape
changes
contributions.
discover
while
cell
divisions
drive
expansion
along
one
axis,
their
contribution
orthogonal
axis
is
cancelled
by
rearrangements
changes.
This
finding
raises
possibility
anisotropic
mechanical
constraints
contribute
orientation
disc.
Annual Review of Cell and Developmental Biology,
Год журнала:
2022,
Номер
38(1), С. 321 - 347
Опубликована: Май 14, 2022
Patterns
are
ubiquitous
in
living
systems
and
underlie
the
dynamic
organization
of
cells,
tissues,
embryos.
Mathematical
frameworks
have
been
devised
to
account
for
self-organization
biological
patterns,
most
famously
Turing
framework.
can
be
defined
space,
example,
form
stripes;
time,
such
as
during
oscillations;
or
both,
traveling
waves.
The
formation
these
patterns
different
origins:
purely
chemical,
mechanical,
a
combination
two.
Beyond
variety
molecular
implementations
we
emphasize
unitary
principles
associated
with
them,
across
scales
space
within
general
mechanochemical
We
illustrate
where
mechanisms
pattern
arise
from
cellular
tissue
scales,
an
emphasis
on
morphogenesis.
Our
goal
is
convey
picture
that
draws
attention
rather
than
solely
specific
mechanisms.
The Journal of Cell Biology,
Год журнала:
2022,
Номер
221(4)
Опубликована: Март 7, 2022
Epithelial
cell–cell
junctions
remodel
in
response
to
mechanical
stimuli
maintain
barrier
function.
Previously,
we
found
that
local
leaks
tight
(TJs)
are
rapidly
repaired
by
local,
transient
RhoA
activation,
termed
“Rho
flares,”
but
how
Rho
flares
regulated
is
unknown.
Here,
discovered
intracellular
calcium
flashes
and
junction
elongation
early
events
the
flare
pathway.
Both
laser-induced
naturally
occurring
TJ
breaks
lead
at
site
of
leaks.
Additionally,
induced
optogenetics
increases
frequency,
suggesting
mechanically
triggered.
Depletion
or
inhibition
mechanosensitive
channels
(MSCs)
reduces
amplitude
diminishes
sustained
activation
flares.
MSC-dependent
influx
necessary
global
function
regulating
reinforcement
proteins
via
contraction.
In
all,
uncovered
a
novel
role
for
remodeling,
allowing
epithelial
cells
repair
stimuli.
Nature Communications,
Год журнала:
2019,
Номер
10(1)
Опубликована: Фев. 13, 2019
Abstract
In
plants
mechanical
signals
pattern
morphogenesis
through
the
polar
transport
of
hormone
auxin
and
regulation
interphase
microtubule
(MT)
orientation.
To
date,
mechanisms
by
which
such
induce
changes
in
cell
polarity
remain
unknown.
Through
a
combination
time-lapse
imaging,
chemical
perturbations,
we
show
that
stimulation
SAM
causes
transient
cytoplasmic
calcium
ion
concentration
(Ca
2+
)
Ca
response
is
required
for
downstream
PIN-FORMED
1
(PIN1)
polarity.
We
also
find
dynamic
occur
during
development
this
PIN1
polarity,
though
not
sufficient.
contrast,
necessary
MTs
to
perturbations
revealing
specifically
acts
mechanics
regulate
response.
