Natural products in drug discovery: advances and opportunities

Nature Reviews Drug Discovery, Год журнала: 2021, Номер 20(3), С. 200 - 216

Опубликована: Янв. 28, 2021

Язык: Английский

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Role of Insect Gut Microbiota in Pesticide Degradation: A Review

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Май 3, 2022

Insect pests cause significant agricultural and economic losses to crops worldwide due their destructive activities. Pesticides are designed be poisonous intentionally released into the environment combat menace caused by these noxious pests. To survive, insects can resist toxic substances introduced humans in form of pesticides. According recent findings, microbes that live insect as symbionts have recently been found protect hosts against toxins. Symbioses formed between various microbes, a defensive mechanism pathogens Insects’ guts provide unique conditions for microbial colonization, resident bacteria deliver numerous benefits hosts. Insects vary significantly reliance on gut basic functions. digestive tracts very different shape chemical properties, which big impact structure composition community. microbiota has contribute feeding, parasite pathogen protection, immune response modulation, pesticide breakdown. The current review will examine roles detoxification mechanisms behind development resistance better understand detoxifying agriculturally pest insects, we provided comprehensive information regarding role

Язык: Английский

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Origin and function of beneficial bacterial symbioses in insects

Nature Reviews Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

Язык: Английский

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Stingless Bee Larvae Require Fungal Steroid to Pupate

Scientific Reports, Год журнала: 2018, Номер 8(1)

Опубликована: Янв. 12, 2018

The larval stage of the stingless bee Scaptotrigona depilis must consume a specific brood cell fungus in order to continue development. Here we show that this is member genus Zygosaccharomyces and provides essential steroid precursors developing bee. Insect pupation requires ecdysteroid hormones, as insects cannot synthesize sterols de novo, they obtain steroids their diet. Larval vitro culturing assays demonstrated consuming ergosterol recapitulates developmental effects on S. ingestion sp. cells. Thus, determined molecular underpinning intimate mutualistic symbiosis. Phylogenetic analyses showed similar cases bee-Zygosaccharomyces symbiosis may exist. This unprecedented case bee-fungus driven by requirement brings new perspectives regarding pollinator-microbiota interaction preservation.

Язык: Английский

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Nutrient-Dependent Impact of Microbes onDrosophila suzukiiDevelopment

mBio, Год журнала: 2018, Номер 9(2)

Опубликована: Март 19, 2018

ABSTRACT Drosophila suzukii Matsumura is an invasive species of vinegar fly that has become a prominent pest berries and other soft-skinned fruits. Unlike most species, female D. flies lay their eggs in ripening ripe fruits larvae develop within the fruit. To understand how utilize fruits, which usually have low levels protein, we investigated microbiota field-captured laboratory-reared further examined combined influence diet microbes on host fitness. Field-captured were associated with diverse microbiota, varied significantly sampling location season. In contrast, possessed strikingly lower bacterial abundance diversity. A comparison conventionally reared (CR) germ-free (GF) revealed does not alter its development but decreases life span under conditions nutrient-sufficient diet. However, essential for strawberry-based or blueberry-based fruit diets. This developmental failure could be rescued by reassociation single fungal addition high quantity heat-killed microbes. addition, found proteins are limiting respect to fruit-based diets GF show signs protein starvation. Taken together, our study results demonstrate provides key required fresh Our work shows impact fitness depends strongly nutritional conditions. IMPORTANCE Animals commonly specific microbes, play important roles little information about function been available , also known as Spotted wing drosophila. structure affected environment, where variable depending situation. For instance, presence deleterious growing protein-rich yet beneficial protein-poor Additionally, must feed obtain necessary larval strawberries blueberries. report validates complexity seen host-microbe interactions may provide useful control.

Язык: Английский

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Biosynthetic gene clusters and the evolution of fungal chemodiversity

Natural Product Reports, Год журнала: 2020, Номер 37(7), С. 868 - 878

Опубликована: Янв. 1, 2020

This highlight synthesizes knowledge of the molecular evolutionary processes – functional divergence, horizontal transfer, andde novoassembly that govern biosynthetic gene cluster diversification and generation chemodiversity in fungi.

Язык: Английский

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An antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in Lagria villosa beetles

Nature Communications, Год журнала: 2018, Номер 9(1)

Опубликована: Июнь 20, 2018

Microbial symbionts are often a source of chemical novelty and can contribute to host defense against antagonists. However, the ecological relevance mediators remains unclear for most systems. Lagria beetles live in symbiosis with multiple strains Burkholderia bacteria that protect their offspring pathogens. Here, we describe antifungal polyketide lagriamide, provide evidence supporting it is produced by an uncultured symbiont, gladioli Lv-StB, which dominant field-collected villosa. Interestingly, lagriamide structurally similar bistramides, defensive compounds found marine tunicates. We identify gene cluster probably involved biosynthesis, horizontal acquisition these genes, show naturally occurring symbiont on egg protective soil environment. Our findings highlight potential microbial transfer as influential sources innovation.

Язык: Английский

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Charting an Unexplored Streptococcal Biosynthetic Landscape Reveals a Unique Peptide Cyclization Motif

Journal of the American Chemical Society, Год журнала: 2018, Номер 140(50), С. 17674 - 17684

Опубликована: Ноя. 6, 2018

Peptide natural products are often used as signals or antibiotics and contain unusual structural modifications, thus providing opportunities for expanding our understanding of Nature's therapeutic biosynthetic repertoires. Herein, we have investigated the under-explored potential Streptococci, prevalent bacteria in mammalian microbiomes that include mutualistic, commensal, pathogenic members. Using a new bioinformatic search strategy, which linked versatile radical S-adenosylmethionine (RaS) enzyme superfamily to an emerging class context quorum sensing control, identified numerous, uncharted loci. Focusing on one such locus, unprecedented post-translational modification, consisting tetrahydro[5,6]benzindole cyclization motif four unactivated positions by two C–C bonds regio- stereospecific manner single RaS enzyme. Our results expand scope reactions microbes at their disposal concocting complex ribosomal peptides.

Язык: Английский

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Convergent evolution of complex structures for ant–bacterial defensive symbiosis in fungus-farming ants

Proceedings of the National Academy of Sciences, Год журнала: 2018, Номер 115(42), С. 10720 - 10725

Опубликована: Окт. 3, 2018

Evolutionary adaptations for maintaining beneficial microbes are hallmarks of mutualistic evolution. Fungus-farming “attine” ant species have complex cuticular modifications and specialized glands that house nourish antibiotic-producing Actinobacteria symbionts, which in turn protect their hosts’ fungus gardens from pathogens. Here we reconstruct ant–Actinobacteria evolutionary history across the full range variation within subtribe Attina by combining dated phylogenomic ultramorphological analyses. Ancestral-state analyses indicate symbiosis arose early attine-ant evolution, a conclusion consistent with direct observations on fossil ants Oligo-Miocene amber. qPCR indicates dominant ant-associated belong to genus Pseudonocardia . Tracing trajectories -maintaining mechanisms attine reveals continuum adaptations. In Myrmicocrypta species, retain many ancestral morphological behavioral traits, occur specific locations legs antennae, unassociated any structures. contrast, structures, including crypts tubercles, evolved at least three times derived lineages. Conspicuous caste differences structures present worker queens but reduced or lost males, vertical transmission. Although majority associated , there been multiple losses bacterial symbionts bacteria-maintaining different lineages over time. The origin ant– mutualism convergences strikingly similar anatomical played critical role evolution fungiculture.

Язык: Английский

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Gut bacteria of field-collected larvae of Spodoptera frugiperda undergo selection and are more diverse and active in metabolizing multiple insecticides than laboratory-selected resistant strains

Journal of Pest Science, Год журнала: 2020, Номер 93(2), С. 833 - 851

Опубликована: Янв. 30, 2020

Язык: Английский

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