Molecules,
Год журнала:
2021,
Номер
26(11), С. 3311 - 3311
Опубликована: Май 31, 2021
In
the
natural
environment,
interactions
between
species
are
a
common
phenomena.
The
mechanisms
of
interaction
different
mainly
studied
using
genomic,
transcriptomic,
proteomic,
and
metabolomic
techniques.
Metabolomics
is
crucial
part
system
biology
based
on
precision
instrument
analysis.
last
decade,
emerging
field
metabolomics
has
received
extensive
attention.
not
only
provides
qualitative
quantitative
method
for
studying
species,
but
also
helps
clarify
defense
host
pathogen,
to
explore
new
metabolites
with
various
biological
activities.
This
review
focuses
methods
progress
interspecies
metabolomics.
Additionally,
prospects
challenges
discussed.
Critical Reviews in Analytical Chemistry,
Год журнала:
2020,
Номер
52(4), С. 712 - 734
Опубликована: Окт. 7, 2020
Metabolomics
is
a
young
field
of
knowledge
that
arises
linked
to
other
omics
such
as
genomics,
transcriptomics,
and
proteomics.
This
discipline
seeks
understand
the
performance
metabolites,
identifying,
quantifying
them,
thus
understanding
its
mechanism
action.
new
branch
science
shows
high
potential,
due
noninvasive
character
close
relation
with
phenotype.
Several
techniques
have
been
developed
study
metabolome
biological
samples,
fundamentally
nuclear
magnetic
resonance
(NMR),
mass
spectrometry
(MS)
vibrational
(VS)
or
combination
several
techniques.
These
are
focused
separate,
detect,
characterize,
quantify
well
elucidate
their
structures
function
on
metabolic
pathways
they
involved.
However,
complexity
metabolome,
in
most
cases
it
necessary
apply
these
completely
whole
scenery.
review
aimed
offer
summary
current
analytical
for
metabolomics
application
different
fields
environmental,
food
health
sciences.
Each
technique
advantages
drawbacks
depending
technical
characteristics
limitations,
some
factors,
aim
nature
sample
will
condition
choice.
Regarding
applications,
NMR
has
employed
specially
identify
compounds
structures.
The
use
MS
gained
popularity
because
versatility,
easiness
be
coupled
separation
sensitivity.
Whereas
VS
widely
situ
studies,
nondestructive
character.
applications
growing
each
year,
advances
allow
increase
comprehension
processes.
Further
development
tools
continue
exploiting
all
possibilities
metabolomics.
HighlightsMetabolomics
metabolites
actionDifferent
improved
last
yearsMetabolomics
cover
clinical,
pharmaceuticals
environmental
sciencesThis
Analytical Chemistry,
Год журнала:
2020,
Номер
92(22), С. 14867 - 14871
Опубликована: Ноя. 2, 2020
Metabolomics
plays
a
pivotal
role
in
systems
biology,
and
NMR
is
central
tool
with
high
precision
exceptional
resolution
of
chemical
information.
Most
metabolomic
studies
are
based
on
1H
1D
spectroscopy,
severely
limited
by
peak
overlap.
13C
benefits
from
larger
signal
dispersion
but
barely
used
metabolomics
due
to
ca.
6000-fold
lower
sensitivity.
We
introduce
new
approach,
hyperpolarized
at
natural
abundance,
that
circumvents
this
limitation.
A
untargeted
NMR-based
workflow
dissolution
dynamic
nuclear
polarization
(d-DNP)
for
the
first
time
enabled
abundance
metabolomics.
Statistical
analysis
resulting
data
distinguishes
two
groups
plant
(tomato)
extracts
highlights
biomarkers,
full
agreement
previous
results
same
biological
model.
also
optimize
parameters
semiautomated
d-DNP
system
suitable
high-throughput
studies.
Analytical Chemistry,
Год журнала:
2021,
Номер
93(7), С. 3542 - 3550
Опубликована: Фев. 8, 2021
The
combination
of
ion-mobility
(IM)
separation
with
mass
spectrometry
(MS)
has
impacted
global
measurement
efforts
in
areas
ranging
from
food
analysis
to
drug
discovery.
Reasons
for
the
broad
adoption
IM-MS
include
its
significantly
increased
peak
capacity,
duty-cycle,
and
ability
reconstruct
fragmentation
data
parallel,
all
which
greatly
enable
analyses
complex
mixtures.
More
fundamentally,
however,
measurements
ion-gas
molecule
collision
cross
sections
(CCSs)
are
used
support
compound
identification
quantitation
as
well
study
structures
large
biomolecules.
As
first
commercialized
form
IM-MS,
Traveling
Wave
Ion
Mobility
(TWIM)
devices
operated
at
low
pressures
(∼3
mbar)
voltages,
relatively
short
(∼25
cm),
separate
ions
on
a
timescale
tens
milliseconds.
These
qualities
make
TWIM
ideally
suited
hybridization
MS.
Owing
complicated
motion
devices,
IM
transit
times
must
be
calibrated
CCS
measurements.
Applicability
these
calibrations
hitherto
been
restricted
primarily
singly
charged
small
molecules
some
classes
large,
multiply
under
narrower
range
instrument
conditions.
Here,
we
introduce
extensively
characterize
dramatically
improved
calibration
methodology.
Using
over
2500
experimental
sets,
covering
that
span
3.5
orders
magnitude
molecular
mass,
demonstrate
robust
expanded
conditions,
thereby
opening
up
new
analytical
application
enabling
expansion
high-precision
both
existing
next-generation
instrumentation.
Current Opinion in Food Science,
Год журнала:
2021,
Номер
40, С. 168 - 178
Опубликована: Апрель 19, 2021
Milk
is
a
foodstuff
widely
consumed
around
the
world
originating
from
variety
of
different
species,
animal
management
and
production
systems.
In
recent
years,
consumers
have
placed
much
greater
emphasis
on
authenticity
origin
some
food
products
often
willing
to
pay
premium
price
for
such
that
is,
example
'Grass-Fed
Dairy'.
Therefore,
it
important
establish
methods
assess
both
quality
authentication
milk
dairy
increased
security
consumer
protection.
Accordingly,
NMR-based,
GC–MS-based,
LC–MS-based
metabolomics
been
established
as
useful
tools
in
analysis
products,
raw
processed
milk.
This
short-review
provides
an
updated
critical
overview
most
metabolomics-based
platforms
multivariate
statistical
available
metabolomic
data
interpretation.
Molecules,
Год журнала:
2021,
Номер
26(11), С. 3311 - 3311
Опубликована: Май 31, 2021
In
the
natural
environment,
interactions
between
species
are
a
common
phenomena.
The
mechanisms
of
interaction
different
mainly
studied
using
genomic,
transcriptomic,
proteomic,
and
metabolomic
techniques.
Metabolomics
is
crucial
part
system
biology
based
on
precision
instrument
analysis.
last
decade,
emerging
field
metabolomics
has
received
extensive
attention.
not
only
provides
qualitative
quantitative
method
for
studying
species,
but
also
helps
clarify
defense
host
pathogen,
to
explore
new
metabolites
with
various
biological
activities.
This
review
focuses
methods
progress
interspecies
metabolomics.
Additionally,
prospects
challenges
discussed.
