Journal of the American Chemical Society,
Год журнала:
2022,
Номер
144(48), С. 22067 - 22074
Опубликована: Ноя. 23, 2022
Terpene
cyclases
(TCs),
extraordinary
enzymes
that
create
the
structural
diversity
seen
in
terpene
natural
products,
are
traditionally
divided
into
two
classes,
class
I
and
II.
Although
mechanistic
features
of
TCs
well-known,
corresponding
details
II
counterparts
have
not
been
fully
characterized.
Here,
we
report
genome
mining
discovery
characterization
sesquiterpene
(STCs)
from
Streptomyces.
These
drimenyl
diphosphate
synthases
(DMSs)
first
STCs
shown
to
possess
β,γ-didomain
architecture.
High-resolution
X-ray
crystal
structures
DMS
Streptomyces
showdoensis
(SsDMS)
complex
with
both
a
farnesyl
Mg2+
unveiled
an
induced-fit
mechanism,
unprecedented
binding
mode,
finally
solving
one
lingering
questions
TC
enzymology.
This
study
supports
continued
for
novel
bacterial
provides
new
insights
canonical
will
lead
advances
engineering
synthetic
biology.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(33), С. 18161 - 18181
Опубликована: Авг. 8, 2023
Bolstered
by
recent
advances
in
bioinformatics,
genetics,
and
enzyme
engineering,
the
field
of
chemoenzymatic
synthesis
has
enjoyed
a
rapid
increase
popularity
utility.
This
Perspective
explores
integration
enzymes
into
multistep
chemical
syntheses,
highlighting
unique
potential
biocatalytic
transformations
to
streamline
complex
natural
products.
In
particular,
we
identify
four
primary
conceptual
approaches
illustrate
each
with
number
landmark
case
studies.
Future
opportunities
challenges
are
also
discussed.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(13), С. 6879 - 6879
Опубликована: Июнь 22, 2024
Phytopathogenic
fungi
are
responsible
for
diseases
in
commercially
important
crops
and
cause
major
supply
problems
the
global
food
chain.
Plants
were
able
to
protect
themselves
from
disease
before
humans
played
an
active
role
protecting
plants.
They
known
synthesize
a
variety
of
secondary
metabolites
(SMs),
such
as
terpenes,
alkaloids,
phenolic
compounds,
which
can
be
extracted
using
conventional
unconventional
techniques
formulate
biofungicides;
plant
extracts
have
antifungal
activity
various
mechanisms
action
against
these
organisms.
In
addition,
they
considered
non-phytotoxic
potentially
effective
control.
sustainable
economically
viable
alternative
use
agriculture,
is
why
biofungicides
increasingly
recognized
attractive
option
solve
caused
by
synthetic
fungicides.
Currently,
organic
farming
continues
grow,
highlighting
importance
developing
environmentally
friendly
alternatives
crop
production.
This
review
provides
compilation
literature
on
biosynthesis,
phytopathogens,
extraction
formulation
biofungicides,
biological
phytopathogenic
fungi,
regulation,
advantages,
disadvantages
overview
current
agriculture.
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Апрель 19, 2025
Terpenoids
are
the
largest
family
of
natural
products
but
relatively
rare
in
bacteria.
Genome
mining
reveals
widespread
prevalence
terpene
synthases,
enzymes
responsible
for
constructing
hydrocarbon
skeletons,
Here,
we
show
that
125
(37%)
334
synthases
from
8
phyla,
17
classes,
and
83
genera
bacteria
active
as
diterpene
synthases.
Isolation
structural
elucidation
28
diterpenes
31
reveal
three
previously
unreported
skeletons
other
organisms
with
unknown
biosynthetic
pathways,
known
to
best
our
knowledge
not
seen
bacteria,
or
new
stereochemical
isomers
diterpenes.
We
also
identify
type
I
myxobacteria
cyanobacteria.
This
study
will
help
discover
products,
advance
studies
terpenoid
biosynthesis
enzymology,
provide
model
systems
probe
ecological
roles
terpenes.
Natural Product Reports,
Год журнала:
2020,
Номер
38(3), С. 566 - 585
Опубликована: Окт. 1, 2020
Covering:
up
to
July
2020Fungal
meroterpenoid
cyclases
are
a
recently
discovered
emerging
family
of
membrane-integrated,
non-canonical
terpene
cyclases.
They
catalyze
the
conversion
hybrid
isoprenic
precursors
towards
complex
scaffolds
and
therefore
great
importance
in
structure
diversification
biosynthesis.
The
products
these
pathways
exhibit
intriguing
molecular
highly
potent
bioactivities,
making
them
privileged
structures
from
Nature
attractive
candidates
for
drug
development
or
industrial
applications.
This
review
will
provide
comprehensive
comparative
view
on
fungal
cyclases,
their
chemistries
scaffold
formation
step
polycyclic
natural
products.
Proceedings of the National Academy of Sciences,
Год журнала:
2021,
Номер
118(29)
Опубликована: Июль 13, 2021
Significance
Chimeric
terpene
synthases,
termed
PTTSs,
are
a
unique
family
of
enzymes
occurring
only
in
fungi.
Characterizing
PTTSs
is
challenging
due
to
the
complex
reactions
they
catalyze
and
structural
complexity
their
products.
Here,
by
devising
an
efficient
precursor-providing
yeast
chassis
incorporating
high-throughput
automated
platform,
we
identified
34
active
which
was
considerably
more
than
number
known
functional
PTTSs.
This
effective
rapid
pipeline
can
be
employed
for
characterization
other
or
related
terpenoid
biosynthetic
enzymes.
By
systematically
analyzing
presence/absence
PTTS
genes
together
with
phylogenetic
analysis,
ancestral
gene
inferred
have
undergone
duplication
divergence,
led
development
two
distinct
cyclization
mechanisms.
FEMS Microbiology Reviews,
Год журнала:
2023,
Номер
47(2)
Опубликована: Март 1, 2023
Abstract
Terpenoids,
also
known
as
isoprenoids,
are
the
largest
and
most
diverse
class
of
organic
compounds
in
nature
involved
many
membrane-associated
cellular
processes,
including
membrane
organization,
electron
transport
chain,
cell
signaling,
phototrophy.
Terpenoids
ancient
with
their
origin
presumably
before
last
universal
common
ancestor.
However,
Bacteria
Archaea
to
possess
two
distinct
terpenoid
repertoires
utilize
terpenoids
differently.
Most
notably,
archaea
constitute
solely
made
terpenoid-based
phospholipids,
contrary
bacterial
that
consists
fatty
acid-based
phospholipids.
Thus,
composition
ancestral
membranes
at
beginning
life
diversification
early
remain
enigmatic.
This
review
addresses
these
key
issues
through
comprehensive
phylogenomic
analyses
extant
biosynthesis
enzymes
Archaea.
We
aim
infer
basal
components
machinery
have
an
divergence
domains
shed
light
on
deep
evolutionary
connection
between
biochemistry
life.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(13), С. 11184 - 11184
Опубликована: Июль 6, 2023
Filamentous
fungi
are
one
of
the
most
important
producers
secondary
metabolites.
Some
them
can
have
a
toxic
effect
on
human
body,
leading
to
diseases.
On
other
hand,
they
widely
used
as
pharmaceutically
significant
drugs,
such
antibiotics,
statins,
and
immunosuppressants.
A
single
fungus
species
in
response
various
signals
produce
100
or
more
Such
signaling
is
possible
due
coordinated
regulation
several
dozen
biosynthetic
gene
clusters
(BGCs),
which
mosaically
localized
different
regions
fungal
chromosomes.
Their
includes
levels,
from
pathway-specific
regulators,
whose
genes
inside
BGCs,
global
regulators
cell
(taking
into
account
changes
pH,
carbon
consumption,
etc.)
metabolism
(affecting
epigenetic
driven
by
velvet
family
proteins,
LaeA,
etc.).
In
addition,
low-molecular-weight
substances
mediating
regulatory
processes.
This
review
devoted
critical
analysis
available
data
"turning
on"
"off"
biosynthesis
metabolites
filamentous
fungi.
To
describe
ongoing
processes,
model
"piano
regulation"
proposed,
whereby
pressing
certain
key
(signal)
leads
extraction
sound
"musical
instrument
cell",
expressed
production
specific
metabolite.
