Discovery, Structure, and Mechanism of a Class II Sesquiterpene Cyclase DOI
Xingming Pan,

Wenyu Du,

Xiaowei Zhang

и другие.

Journal of the American Chemical Society, Год журнала: 2022, Номер 144(48), С. 22067 - 22074

Опубликована: Ноя. 23, 2022

Terpene cyclases (TCs), extraordinary enzymes that create the structural diversity seen in terpene natural products, are traditionally divided into two classes, class I and II. Although mechanistic features of TCs well-known, corresponding details II counterparts have not been fully characterized. Here, we report genome mining discovery characterization sesquiterpene (STCs) from Streptomyces. These drimenyl diphosphate synthases (DMSs) first STCs shown to possess β,γ-didomain architecture. High-resolution X-ray crystal structures DMS Streptomyces showdoensis (SsDMS) complex with both a farnesyl Mg2+ unveiled an induced-fit mechanism, unprecedented binding mode, finally solving one lingering questions TC enzymology. This study supports continued for novel bacterial provides new insights canonical will lead advances engineering synthetic biology.

Язык: Английский

Charting the Evolution of Chemoenzymatic Strategies in the Syntheses of Complex Natural Products DOI
Carter N. Stout,

Nour Wasfy,

Fang Chen

и другие.

Journal of the American Chemical Society, Год журнала: 2023, Номер 145(33), С. 18161 - 18181

Опубликована: Авг. 8, 2023

Bolstered by recent advances in bioinformatics, genetics, and enzyme engineering, the field of chemoenzymatic synthesis has enjoyed a rapid increase popularity utility. This Perspective explores integration enzymes into multistep chemical syntheses, highlighting unique potential biocatalytic transformations to streamline complex natural products. In particular, we identify four primary conceptual approaches illustrate each with number landmark case studies. Future opportunities challenges are also discussed.

Язык: Английский

Процитировано

24

Expanding the structural diversity of terpenes by synthetic biology approaches DOI
Rong Chen, Ming Wang, Jay D. Keasling

и другие.

Trends in biotechnology, Год журнала: 2024, Номер 42(6), С. 699 - 713

Опубликована: Янв. 16, 2024

Язык: Английский

Процитировано

16

Biofungicides Based on Plant Extracts: On the Road to Organic Farming DOI Open Access
Antonio de Jesús Cenobio-Galindo, Alma Delia Hernández-Fuentes, Uriel González-Lemus

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(13), С. 6879 - 6879

Опубликована: Июнь 22, 2024

Phytopathogenic fungi are responsible for diseases in commercially important crops and cause major supply problems the global food chain. Plants were able to protect themselves from disease before humans played an active role protecting plants. They known synthesize a variety of secondary metabolites (SMs), such as terpenes, alkaloids, phenolic compounds, which can be extracted using conventional unconventional techniques formulate biofungicides; plant extracts have antifungal activity various mechanisms action against these organisms. In addition, they considered non-phytotoxic potentially effective control. sustainable economically viable alternative use agriculture, is why biofungicides increasingly recognized attractive option solve caused by synthetic fungicides. Currently, organic farming continues grow, highlighting importance developing environmentally friendly alternatives crop production. This review provides compilation literature on biosynthesis, phytopathogens, extraction formulation biofungicides, biological phytopathogenic fungi, regulation, advantages, disadvantages overview current agriculture.

Язык: Английский

Процитировано

11

Exploring and expanding the natural chemical space of bacterial diterpenes DOI Creative Commons

Xiuting Wei,

Wenbo Ning, Caitlin A McCadden

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 19, 2025

Terpenoids are the largest family of natural products but relatively rare in bacteria. Genome mining reveals widespread prevalence terpene synthases, enzymes responsible for constructing hydrocarbon skeletons, Here, we show that 125 (37%) 334 synthases from 8 phyla, 17 classes, and 83 genera bacteria active as diterpene synthases. Isolation structural elucidation 28 diterpenes 31 reveal three previously unreported skeletons other organisms with unknown biosynthetic pathways, known to best our knowledge not seen bacteria, or new stereochemical isomers diterpenes. We also identify type I myxobacteria cyanobacteria. This study will help discover products, advance studies terpenoid biosynthesis enzymology, provide model systems probe ecological roles terpenes.

Язык: Английский

Процитировано

2

Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis DOI
Benjamin R. Lichman, Mohamed O. Kamileen, Gabriel R. Titchiner

и другие.

Nature Chemical Biology, Год журнала: 2018, Номер 15(1), С. 71 - 79

Опубликована: Ноя. 30, 2018

Язык: Английский

Процитировано

82

Chemistry of fungal meroterpenoid cyclases DOI
Lena Barra, Ikuro Abe

Natural Product Reports, Год журнала: 2020, Номер 38(3), С. 566 - 585

Опубликована: Окт. 1, 2020

Covering: up to July 2020Fungal meroterpenoid cyclases are a recently discovered emerging family of membrane-integrated, non-canonical terpene cyclases. They catalyze the conversion hybrid isoprenic precursors towards complex scaffolds and therefore great importance in structure diversification biosynthesis. The products these pathways exhibit intriguing molecular highly potent bioactivities, making them privileged structures from Nature attractive candidates for drug development or industrial applications. This review will provide comprehensive comparative view on fungal cyclases, their chemistries scaffold formation step polycyclic natural products.

Язык: Английский

Процитировано

57

Systematic mining of fungal chimeric terpene synthases using an efficient precursor-providing yeast chassis DOI Creative Commons
Rong Chen, Qidong Jia, Xin Mu

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2021, Номер 118(29)

Опубликована: Июль 13, 2021

Significance Chimeric terpene synthases, termed PTTSs, are a unique family of enzymes occurring only in fungi. Characterizing PTTSs is challenging due to the complex reactions they catalyze and structural complexity their products. Here, by devising an efficient precursor-providing yeast chassis incorporating high-throughput automated platform, we identified 34 active which was considerably more than number known functional PTTSs. This effective rapid pipeline can be employed for characterization other or related terpenoid biosynthetic enzymes. By systematically analyzing presence/absence PTTS genes together with phylogenetic analysis, ancestral gene inferred have undergone duplication divergence, led development two distinct cyclization mechanisms.

Язык: Английский

Процитировано

43

First trans-eunicellane terpene synthase in bacteria DOI Creative Commons
Zining Li, Baofu Xu, Volga Kojasoy

и другие.

Chem, Год журнала: 2023, Номер 9(3), С. 698 - 708

Опубликована: Янв. 2, 2023

Язык: Английский

Процитировано

24

Four billion years of microbial terpenome evolution DOI
Yosuke Hoshino, Laura Villanueva

FEMS Microbiology Reviews, Год журнала: 2023, Номер 47(2)

Опубликована: Март 1, 2023

Abstract Terpenoids, also known as isoprenoids, are the largest and most diverse class of organic compounds in nature involved many membrane-associated cellular processes, including membrane organization, electron transport chain, cell signaling, phototrophy. Terpenoids ancient with their origin presumably before last universal common ancestor. However, Bacteria Archaea to possess two distinct terpenoid repertoires utilize terpenoids differently. Most notably, archaea constitute solely made terpenoid-based phospholipids, contrary bacterial that consists fatty acid-based phospholipids. Thus, composition ancestral membranes at beginning life diversification early remain enigmatic. This review addresses these key issues through comprehensive phylogenomic analyses extant biosynthesis enzymes Archaea. We aim infer basal components machinery have an divergence domains shed light on deep evolutionary connection between biochemistry life.

Язык: Английский

Процитировано

22

Fungal BGCs for Production of Secondary Metabolites: Main Types, Central Roles in Strain Improvement, and Regulation According to the Piano Principle DOI Open Access
Alexander A. Zhgun

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(13), С. 11184 - 11184

Опубликована: Июль 6, 2023

Filamentous fungi are one of the most important producers secondary metabolites. Some them can have a toxic effect on human body, leading to diseases. On other hand, they widely used as pharmaceutically significant drugs, such antibiotics, statins, and immunosuppressants. A single fungus species in response various signals produce 100 or more Such signaling is possible due coordinated regulation several dozen biosynthetic gene clusters (BGCs), which mosaically localized different regions fungal chromosomes. Their includes levels, from pathway-specific regulators, whose genes inside BGCs, global regulators cell (taking into account changes pH, carbon consumption, etc.) metabolism (affecting epigenetic driven by velvet family proteins, LaeA, etc.). In addition, low-molecular-weight substances mediating regulatory processes. This review devoted critical analysis available data "turning on" "off" biosynthesis metabolites filamentous fungi. To describe ongoing processes, model "piano regulation" proposed, whereby pressing certain key (signal) leads extraction sound "musical instrument cell", expressed production specific metabolite.

Язык: Английский

Процитировано

20