Nanomechanical mapping of soft materials with the atomic force microscope: methods, theory and applications

Chemical Society Reviews, Год журнала: 2020, Номер 49(16), С. 5850 - 5884

Опубликована: Янв. 1, 2020

This review provides an introduction to the state-of-the-art force microscope methods map at high-spatial resolution elastic and viscoelastic properties of proteins, polymers cells.

Язык: Английский

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The extracellular matrix viscoelasticity as a regulator of cell and tissue dynamics

Current Opinion in Cell Biology, Год журнала: 2021, Номер 72, С. 10 - 18

Опубликована: Май 13, 2021

Язык: Английский

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Mechanical properties of human tumour tissues and their implications for cancer development

Nature Reviews Physics, Год журнала: 2024, Номер 6(4), С. 269 - 282

Опубликована: Март 19, 2024

Язык: Английский

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Measuring the elastic modulus of soft culture surfaces and three-dimensional hydrogels using atomic force microscopy

Nature Protocols, Год журнала: 2021, Номер 16(5), С. 2418 - 2449

Опубликована: Апрель 14, 2021

Язык: Английский

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Best practices and recommendations for accurate nanomechanical characterization of heterogeneous polymer systems with atomic force microscopy

Progress in Polymer Science, Год журнала: 2021, Номер 119, С. 101420 - 101420

Опубликована: Июнь 9, 2021

Язык: Английский

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Intrinsic Mechanical Cues and Their Impact on Stem Cells and Embryogenesis

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 9

Опубликована: Ноя. 8, 2021

Although understanding how soluble cues direct cellular processes revolutionised the study of cell biology in second half 20th century, over last two decades, new insights into mechanical similarly impact fate decisions has gained momentum. During development, extrinsic such as fluid flow, shear stress and compressive forces are essential for normal embryogenesis to proceed. Indeed, both adult embryonic stem cells can respond applied forces, but they also detect intrinsic from their surrounding environment, stiffness extracellular matrix, which impacts differentiation morphogenesis. Cells changes environment using surface receptors integrins focal adhesions. Moreover, dynamic rearrangements cytoskeleton have been identified a key means by transmitted matrix vice versa. we some downstream mechanisms whereby translated behaviour, many signalling pathways remain be defined. This review discusses importance on decisions, emerging roles mechano-sensors enabling sense its microenvironment, role intracellular translating transcriptional outputs. In addition, contribution fundamental during apical constriction convergent extension is discussed. The continued development tools measure biomechanical properties soft tissues vivo likely uncover currently underestimated contributions these embryogenesis, may inform regenerative strategies tissue repair.

Язык: Английский

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Ultrasound-assisted covalent reaction of myofibrillar protein: The improvement of functional properties and its potential mechanism

Ultrasonics Sonochemistry, Год журнала: 2021, Номер 76, С. 105652 - 105652

Опубликована: Июнь 24, 2021

The effects of the different combined manner ultrasound and covalent reaction between polyphenol myofibrillar protein (MP) from chicken were studied. More so, antioxidant activities, digestive properties, potential mechanism ultrasound-assisted oxidation system hydrophilic ((−)-Epicatechin gallate, ECG) hydrophobic (Baicalein, BN) polyphenols was also analyzed in this study. Among all treatments, surface hydrophobicity (SUH), active sulfhydryl contents (ASC), specific area (SSA) ultrasonic assisted ECG group (T6) relatively apparent, indicating that a more unfolding MP structure obtained. Furthermore, showed highest activities compared with other treatments on basis results DPPH free radical scavenging metal ion chelating hydroxyl radicals (OH·) activities. simulated digestion kinetic analysis verified had higher bio-accessibility than control group. In contrast, lower digestibility displayed BN summary, might be desirable approach for industrial production better properties.

Язык: Английский

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Mechanical properties of cell sheets and spheroids: the link between single cells and complex tissues

Biophysical Reviews, Год журнала: 2021, Номер 13(4), С. 541 - 561

Опубликована: Июль 13, 2021

Язык: Английский

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Multiscale biomechanics and mechanotransduction from liver fibrosis to cancer

Advanced Drug Delivery Reviews, Год журнала: 2022, Номер 188, С. 114448 - 114448

Опубликована: Июль 9, 2022

Язык: Английский

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Techniques for characterizing mechanical properties of soft tissues

Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials, Год журнала: 2022, Номер 138, С. 105575 - 105575

Опубликована: Ноя. 19, 2022

The characterization of soft tissues remains a vital need for various bioengineering and medical fields. Developing areas such as regenerative medicine, robot-aided surgery, surgical simulations all require accurate knowledge about the mechanical properties to replicate their mechanics. Mechanical can be characterized through several different techniques atomic force microscopy, compression testing, tensile testing. However, many these methods contain considerable differences in ability accurately characterize tissues. As result variations, there are often discrepancies reported values numerous studies. This paper reviews common that have been applied obtain highlights advantages well disadvantages. limitations, accuracies, repeatability, in-vivo testing capability, types measurable each method also discussed.

Язык: Английский

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