Critical Reviews in Biotechnology,
Год журнала:
2021,
Номер
41(7), С. 1023 - 1045
Опубликована: Март 17, 2021
Enzymes
and
cell
factories
play
essential
roles
in
industrial
biotechnology
for
the
production
of
chemicals
fuels.
The
properties
natural
enzymes
cells
often
cannot
meet
requirements
different
processes
terms
cost-effectiveness
high
durability.
To
rapidly
improve
their
performances,
laboratory
evolution
equipped
with
high-throughput
screening
methods
facilities
is
commonly
used
to
tailor
desired
factories,
addressing
challenges
achieving
titer
yield
target
products
at
high/low
temperatures
or
extreme
pH,
unnatural
environments
presence
unconventional
media.
Droplet
microfluidic
(DMFS)
systems
have
demonstrated
great
potential
exploring
vast
genetic
diversity
a
manner
(>106/h)
been
increasingly
recent
years,
contributing
identification
extraordinary
mutants.
This
review
highlights
advances
concepts
DMFS
library
screening,
including
key
factors
droplet
generation
manipulation,
signal
sources
sensitive
detection
sorting,
comprehensive
summary
success
stories
implementation
engineering
during
past
decade.
Frontiers in Chemistry,
Год журнала:
2021,
Номер
9
Опубликована: Апрель 23, 2021
Droplet-based
microfluidics
has
been
widely
applied
in
enzyme
directed
evolution
(DE),
either
cell
or
cell-free
system,
due
to
its
low
cost
and
high
throughput.
As
the
isolation
principles
are
based
on
labeled
label-free
characteristics
droplets,
sorting
method
contributes
mostly
efficiency
of
whole
system.
Fluorescence-activated
droplet
(FADS)
is
but
faces
challenges
target
scope.
Label-free
methods
show
potential
greatly
broaden
microfluidic
application
range.
Here,
we
review
developments
through
a
comprehensive
literature
survey,
including
detections
[FADS
absorbance-activated
(AADS)]
[electrochemical-based
(ECDS),
mass-activated
(MADS),
Raman-activated
(RADS),
nuclear
magnetic
resonance-based
(NMR-DS)].
We
highlight
recent
cases
last
5
years
which
novel
enzymes
highly
efficient
variants
generated
by
DE.
In
addition,
advantages
different
briefly
discussed
provide
an
outlook
for
future
applications
Journal of Energetic Materials,
Год журнала:
2021,
Номер
41(3), С. 411 - 428
Опубликована: Сен. 22, 2021
An
effective
method
is
proposed
for
preparing
energetic
microspheres
by
fluid-focusing
microchannels.
This
combines
the
advantages
of
low
reagent
consumption,
instrument
miniaturization,
accurate
control,
and
efficient
heat
mass
transfer.
The
effects
two-phase
liquid
flow
rate
ratio
water
to
oil
on
droplet
formation
was
fully
studied.
To
verify
applicability
method,
nitroamine
explosives
HMX
(cyclotetramethylene
tetranitramine)
employed
with
fluorine
rubber
(F2602)
as
binder
prepare
HMX/F2602
microsphere,
which
were
compared
samples
prepared
traditional
water-suspension
method.
SEM
data
showed
that
morphology,
particle
size
uniformity,
monodispersity
microfluidic
are
better
than
those
DSC
suspension
apparent
activation
energy
(Ea)
values
increased
25.47
kJ/mol
coated
sample.
Meanwhile,
raw
material
HMX,
H50,
fluidity
bulk
density
significantly
improved
(HMX/F2602
microsphere
HMX:
H50
=
86.9
25.2
cm,
repose
angle
33.1
51.3°,
0.714
0.604
g
cm−3).
study
demonstrates
feasibility
an
efficient,
strategy
explosive
microspheres.
Critical Reviews in Biotechnology,
Год журнала:
2021,
Номер
41(7), С. 1023 - 1045
Опубликована: Март 17, 2021
Enzymes
and
cell
factories
play
essential
roles
in
industrial
biotechnology
for
the
production
of
chemicals
fuels.
The
properties
natural
enzymes
cells
often
cannot
meet
requirements
different
processes
terms
cost-effectiveness
high
durability.
To
rapidly
improve
their
performances,
laboratory
evolution
equipped
with
high-throughput
screening
methods
facilities
is
commonly
used
to
tailor
desired
factories,
addressing
challenges
achieving
titer
yield
target
products
at
high/low
temperatures
or
extreme
pH,
unnatural
environments
presence
unconventional
media.
Droplet
microfluidic
(DMFS)
systems
have
demonstrated
great
potential
exploring
vast
genetic
diversity
a
manner
(>106/h)
been
increasingly
recent
years,
contributing
identification
extraordinary
mutants.
This
review
highlights
advances
concepts
DMFS
library
screening,
including
key
factors
droplet
generation
manipulation,
signal
sources
sensitive
detection
sorting,
comprehensive
summary
success
stories
implementation
engineering
during
past
decade.
