CRISPR-Cas12a-mediated label-free electrochemical aptamer-based sensor for SARS-CoV-2 antigen detection

Bioelectrochemistry, Год журнала: 2022, Номер 146, С. 108105 - 108105

Опубликована: Март 20, 2022

Язык: Английский

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Multiplexed detection of foodborne pathogens using one-pot CRISPR/Cas12a combined with recombinase aided amplification on a finger-actuated microfluidic biosensor

Biosensors and Bioelectronics, Год журнала: 2022, Номер 220, С. 114885 - 114885

Опубликована: Ноя. 5, 2022

Язык: Английский

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An electrochemical biosensor for PD-L1 positive exosomes based on ultra-thin two-dimensional covalent organic framework nanosheets coupled with CRISPR-Cas12a mediated signal amplification

Sensors and Actuators B Chemical, Год журнала: 2022, Номер 362, С. 131813 - 131813

Опубликована: Апрель 6, 2022

Язык: Английский

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A Label-Free Photoelectrochemical Biosensor Based on CRISPR/Cas12a System Responsive Deoxyribonucleic Acid Hydrogel and “Click” Chemistry

ACS Sensors, Год журнала: 2022, Номер 7(10), С. 3153 - 3160

Опубликована: Окт. 11, 2022

A novel label-free photoelectrochemical (PEC) biosensor is presented in this work. As a barrier, the DNA hydrogel could block coupling between g-C3N4 and CdS quantum dots (QDs). Therefore, extremely low photocurrent signals were obtained. The presence of target microRNA-21 can initiate rolling circle amplification (RCA) reaction, which turn produces many repeated sequences to activate CRISPR/Cas12a system. trans-cleavage activity system led degradation hydrogels efficiently. result, g-C3N4/CdS QDs heterojunction was formed through "click" chemistry. Through RCA system, sensitivity PEC improved significantly with detection limit 3.2 aM. proposed sensor also showed excellent selectivity be used detect actual samples. In addition, modular design facilitate different objects. Thus, responsive provides simple, sensitive, flexible way for analysis.

Язык: Английский

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Cas12a/blocker DNA-based multiplex nucleic acid detection system for diagnosis of high-risk human papillomavirus infection

Biosensors and Bioelectronics, Год журнала: 2023, Номер 232, С. 115323 - 115323

Опубликована: Апрель 14, 2023

Язык: Английский

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A Collection of RPA-Based Photoelectrochemical Assays for the Portable Detection of Multiple Pathogens

Analytical Chemistry, Год журнала: 2023, Номер 95(18), С. 7379 - 7386

Опубликована: Апрель 29, 2023

Portable, ultrasensitive, and simultaneously quantitative detection of the nucleic acids multiple foodborne pathogens is critical to public health. However, current testing methods depend on costly equipment tedious amplification steps. In this study, we propose a photoelectrochemical (PEC) biosensor combined with recombinase polymerase (RPA) technology (RPA-PEC) for rapid under irradiation 980 nm light. particular, two working surfaces were designed homemade three-dimensional screen-printed paper-based electrodes. The genomic DNAs Escherichia coli O157:H7 Staphylococcus aureus was initiated by RPA corresponding electrode surfaces, thus forming lab-on-paper platform. Using formed DNA-PEC signaler, photocurrents achieved at 37 °C after only 20 min RPA. performance superior that conventional agarose gel electrophoresis, limits 3.0 7.0 copies/μL E. S. aureus, respectively. Our study pioneers new RPA-PEC method provides directions construction platforms portable acids.

Язык: Английский

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Regulation of Target-activated CRISPR/Cas12a on Surface Binding of Polymer Dots for Sensitive Electrochemiluminescence DNA Analysis

Analytical Chemistry, Год журнала: 2023, Номер 95(18), С. 7396 - 7402

Опубликована: Апрель 29, 2023

Polymer dots (Pdots) have emerged as a type of attractive electrochemiluminescence (ECL) emitter. However, the low ECL efficiency severely limits their practicability. In this work, we develop sensitive biosensing strategy for detection human papilloma virus subtype (HPV-16) DNA by using target-activated CRISPR/Cas12a to regulate binding Pdots-DNA biosensor and local surface plasmon resonance (LSPR) effect electrochemically deposited Au nanoparticles (depAuNPs) enhance emission Pdots bound on biosensor. The is prepared simply assembling hairpin depAuNPs modified electrode. presence target DNA, designed specific can be activated digest single-stranded assistant which decreases amount opened bind surface, thus reduces emission. integration DNA-triggered catalysis LSPR greatly improves sensitivity analysis. Using HPV-16 model, proposed method shows limit (LOD) 3.2 fM at signal-to-noise ratio 3 detectable concentration range 5.0 50 pM. high sensitivity, excellent selectivity, good testing stability, acceptable fabrication reproducibility demonstrate its potential application in bioanalysis.

Язык: Английский

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Recent advances on portable photoelectrochemical biosensors for diagnostics

Electroanalysis, Год журнала: 2023, Номер 35(12)

Опубликована: Окт. 14, 2023

Abstract Efficient and accurate diagnostic methods are important for human health monitoring prevention of major diseases, so the establishment efficient convenient testing clinical diagnosis is urgent. Photoelectrochemical (PEC) biosensors have attracted much attention because their high sensitivity, low detection background, especially advantages combining photoexcitation electrochemical detection. With rapid development integrated circuit technology many wireless transmissions highly aggregated portable micro devices manufactured, its small size, easy to carry, ability visualize results test shown be potential application such devices. This review aims cover recent advances in PEC biosensing within field detection, including principles biosensors, common photovoltaic materials, scalable fabrication techniques biosensing. Future prospects also discussed.

Язык: Английский

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Advances in DNA walking nanomachine‐based biosensors

Deleted Journal, Год журнала: 2024, Номер 2(1)

Опубликована: Янв. 1, 2024

Abstract The field of DNA nanotechnology has evolved beyond the realm controllable movements and randomly shaped nanostructures, now encompassing a diverse array nanomachines, each with unique nanostructures biofunctional attributes. These boast exceptional characteristics such as programmability, integrability, biocompatibility, universality. Among this variety, walking nanomachines have emerged one most prominent nanomotors, distinguished by their ingenious design comprehensive functionality. In recent times, these walkers witnessed remarkable advancements in areas ranging from nanostructural designs to biological applications, including creation sophisticated biosensors capable efficiently detecting tumor‐related biomarkers bioactive substances. This review delves into operational mechanisms which are driven processes protease DNAzyme action well strand displacement photoactivated reactions. It further provides overview different dimensional (1D, 2D, 3D) tracks. A subsequent section introduces biosensing applications electrochemical, optical, other biosensors. concludes forward‐looking perspective on novel challenges developing nanomachine‐based

Язык: Английский

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Split activator of CRISPR/Cas12a for direct and sensitive detection of microRNA

Analytica Chimica Acta, Год журнала: 2024, Номер 1303, С. 342477 - 342477

Опубликована: Март 22, 2024

Язык: Английский

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