Frontiers in Bioengineering and Biotechnology,
Год журнала:
2022,
Номер
10
Опубликована: Июль 22, 2022
Biocatalytic
cascades
play
a
fundamental
role
in
sustainable
chemical
synthesis.
Fusion
enzymes
are
one
of
the
powerful
toolboxes
to
enable
tailored
combination
multiple
for
efficient
cooperative
cascades.
Especially,
this
approach
offers
substantial
potential
practical
application
cofactor-dependent
oxidoreductases
by
forming
cofactor
self-sufficient
Adequate
recycling
while
keeping
oxidized/reduced
confined
microenvironment
benefits
from
fusion
fashion
and
makes
use
harsh
non-aqueous
media
practical.
In
mini-review,
we
have
summarized
various
aqueous
with
focus
on
discussion
linker
design
within
oxidoreductases.
The
properties
reported
linkers
been
reviewed
detail.
Besides,
substrate
loadings
these
studies
listed
showcase
key
limitations
(low
solubility
hydrophobic
substrates)
biocatalysis
when
it
comes
efficiency
economic
feasibility.
Therefore,
straightforward
strategy
applying
has
briefly
discussed
using
oxidoreductase
interest
organic
was
highlighted.
Applied and Environmental Microbiology,
Год журнала:
2024,
Номер
90(2)
Опубликована: Янв. 18, 2024
ATP-dependent
energy-consuming
enzymatic
reactions
are
widely
used
in
cell-free
biocatalysis.
However,
the
direct
addition
of
large
amounts
expensive
ATP
can
greatly
increase
cost,
and
production
is
often
difficult
to
achieve
as
a
result.
Although
polyphosphate
kinase
(PPK)-polyphosphate-based
regeneration
system
has
potential
solve
this
challenge,
generally
poor
thermal
stability
PPKs
limits
widespread
use
method.
In
paper,
we
evaluated
PPK
from
Chemical Communications,
Год журнала:
2023,
Номер
59(23), С. 3451 - 3454
Опубликована: Янв. 1, 2023
We
demonstrate
the
feasibility
of
cellulose
photobiocatalytic
conversion
with
>75%
and
gluconic
acid
selectivity
from
converted
glucose.
This
process
is
realized
via
a
one-pot
sequential
cascade
reaction
by
cellulase
enzymes
carbon
nitride
photocatalyst
that
can
realize
selective
glucose
photoreforming
into
acid.
Cellulase
breakdown
glucose,
which
will
subsequently
be
essential
oxidative
species
(˙O2-
˙OH)
photocatalysis
simultaneous
H2O2
formation.
work
demonstrates
good
example
to
direct
photobiorefining
value-added
chemicals
photo-bio
hybrid
system.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(26), С. 14799 - 14808
Опубликована: Июнь 20, 2024
Monolignols
and
their
derivatives
exhibit
various
pharmaceutical
physiological
characteristics,
such
as
antioxidant
anti-inflammatory
properties.
However,
they
remain
difficult
to
synthesize.
In
this
study,
we
engineered
several
whole-cell
bioconversion
systems
with
carboxylate
reductase
(CAR)-mediated
pathways
for
efficient
synthesis
of
Abstract
The
implementation
of
light‐driven
catalytic
processes
in
biocatalysis
opens
a
golden
window
opportunities.
We
hereby
report
the
merging
photocatalytic
C−C
bond
formation
with
enzymatic
asymmetric
reduction
for
direct
conversion
simple
aldehydes
and
acrylates
or
unsaturated
carboxylic
acids
into
chiral
γ‐lactones.
Tetrabutylammonium
decatungstate
(TBADT)
is
employed
as
photocatalyst
to
trigger
hydroacylation
starting
olefins,
yielding
corresponding
keto
esters/acids.
Subsequently,
an
alcohol
dehydrogenase
converts
intermediate
alcohol,
which
undergoes
lactonization
desired
γ‐lactone.
photochemoenzymatic
synthesis
aliphatic
aromatic
γ‐lactones
was
thereby
achieved
up
>99
%
ee
yield.
This
highlights
power
building
molecular
complexity
by
photocatalysis
access
high‐value
added
compounds
from
simple,
cheap
largely
available
materials.
ACS Sustainable Chemistry & Engineering,
Год журнала:
2023,
Номер
11(28), С. 10284 - 10292
Опубликована: Июль 6, 2023
The
acylations
of
furfurylamine
and
5-hydroxymethylfurfurylamine
(HMFA)
have
been
studied
finding
immobilized
Candida
antarctica
lipase
B
(CALB)
as
an
ideal
biocatalyst.
CALB
was
used
on
two
different
supports
(Novozyme
435
EziG-CALB),
with
the
polymer-coated
controlled
porosity
glass
carrier
material
from
EnginZyme
being
excellent
to
yield
active
stable
enzymatic
preparation
for
acylation
primary
amine
group.
amount
acyl
donor
in
reaction
a
key
factor
achieve
mono-
chemoselective
N-protection
HMFA
large
excess
ethyl
acetate
leading
formation
N,O-diacetylated
product.
Thus,
series
16
nonactivated
esters
were
selectively
modify
group
HMFA,
obtaining
9
hydroxy
amides
under
mild
conditions
quantitative
yields
through
chromatography-free
transformations.
influence
substrate
concentration
studied,
resulting
complete
conversions
all
cases
after
22
h
(100-1000
mM).
Excellent
results
observed
at
100
200
mM
while
higher
concentrations
led
longer
times
and,
some
extent,
diacetylated
product
(up
7%
1
M).
After
this
optimization,
metric
analysis
performed
confirm
high
sustainability
presented
process
(E-factor
1.1
excluding
solvents)
upon
intensification
biotransformation
g
concentration.
possibility
orthogonally
protected
HMFA-derived
amido
has
achieved
clean
sequential
one-pot
using
EziG-CALB,
which
involved
use
methoxy
ester
N-acylation
activated
vinyl
O-protection.
Natural Product Reports,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Recent
progress
in
the
DBTL
cycle,
including
machine
learning,
facilitated
enzyme
mining
for
biocatalysis.
Automation
and
standardization
of
library
construction,
coupled
to
high-throughput
screening,
further
accelerates
discovery
process.
Communications Chemistry,
Год журнала:
2025,
Номер
8(1)
Опубликована: Март 13, 2025
Communications
Chemistry
is
pleased
to
introduce
a
Collection
of
research
works
focused
on
recent
developments
within
the
interdisciplinary
field
chemoenzymatic
synthesis.
Here,
Guest
Editors
highlight
key
themes
and
look
towards
future
this
field.
