Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Март 25, 2025
Perforator
flap
transplantation
is
an
important
technique
in
reconstructive
surgery,
but
necrosis
limits
its
clinical
effectiveness.
Thymoquinone
(TQ),
a
natural
bioactive
plant
quinone
found
black
seed,
exhibits
anti-inflammatory,
angiogenic,
and
antimicrobial
properties.
This
study
investigates
the
therapeutic
effects
of
TQ
perforator
model
through
vivo
vitro
experiments.
Human
umbilical
vein
endothelial
cells
(HUVECs)
were
treated
with
Tert-butyl
Hydroperoxide
(TBHP)
to
simulate
then
TQ.
In
experiments
used
rat
model,
vascularization
was
assessed
using
Doppler
ultrasound
on
days
3
7
after
creation.
On
day
post-surgery,
samples
collected
evaluate
vascularity,
reactive
oxygen
species,
apoptosis
pyroptosis.
Network
pharmacology
analysis
conducted
identify
relevant
signaling
pathways,
molecular
docking
techniques
predict
potential
target
binding
sites.
results
showed
that
both
treatment
NLRP3
inhibitors
reduced
expression
pyroptosis-related
proteins.
indicated
TQ-treated
group
had
increased
survival
area,
blood
flow
intensity,
microvascular
density,
while
oxidative
stress,
apoptosis,
pyroptosis
levels
reduced.
Angiogenesis
enhanced,
SIRT1
protein
increased,
p-P65/NF-κB/NLRP3
pathway
downregulated.
After
inhibitor,
rate
angiogenesis
These
findings
suggest
improves
by
inhibiting
NF-κB/NLRP3
promoting
angiogenesis.
BMC Medical Genomics,
Год журнала:
2025,
Номер
18(1)
Опубликована: Фев. 11, 2025
Ischemic
stroke
(IS)
represents
a
harmful
neurological
disorder
with
limited
treatment
options.
Ferroptosis
accounts
for
the
iron-dependent,
nonapoptotic
cell
death
pattern,
which
shows
feature
of
fatal
lipid
ROS
accumulation.
Nonetheless,
ferroptosis-related
biomarkers
identifying
IS
early
are
currently
lacking.
The
present
study
focused
on
investigating
possible
and
analyzing
their
effects
immune
infiltration.
Altogether
five
hub
differentially
expressed
genes
(DEFRGs)
were
identified
from
relevant
databases.
Additionally,
single-cell
RNA-sequencing
(seq)
analysis
was
conducted
comprehensive
mapping
populations
based
database.
These
DEFRGs
analyzed
using
gene
set
enrichment
analysis,
miRNA
prediction,
RNA-seq
analysis.
A
transient
middle
cerebral
artery
occlusion
mouse
model
constructed.
We
also
adopted
bioinformatics
methods
combined
western
blot,
changes
to
mitochondria,
hematoxylin
&
eosin
staining,
Nissl
fluorescence
immunohistochemistry,
quantitative
real-time
polymerase
chain
reaction
(qRT-PCR)
show
involvement
ferroptosis
in
progression.
results
revealed
that
nuclear
factor
erythroid-derived
2-like
2
(Nfe2l2)
potential
candidate
biomarker
diagnosis,
may
be
suppressed
via
Nfe2l2/HO-1
pathway.
Thus,
drug
targeting
Nfe2l2
can
shed
novel
lights
treatment.
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Март 14, 2025
Ischemic
stroke,
the
most
prevalent
form
of
is
responsible
for
highest
disability
rates
globally
and
ranks
as
primary
cause
mortality
worldwide.
Sirt1,
extensively
investigated
in
neurodegenerative
disorders,
well-known
earliest
member
sirtuins
family.
However,
its
mechanism
action
during
ischemic
stroke
remains
ambiguous.
The
literature
examination
revealed
intricate
involvement
Sirt1
regulating
both
physiological
pathological
mechanisms
stroke.
demonstrates
deacetylation
effects
on
PGC-1α,
HMGB1,
FOXOs,
p53.
It
hinders
activation
NLRP3
inflammasome
NF-κB
while
also
engaging
with
AMPK.
regulates
inflammatory
response,
oxidative
stress,
mitochondrial
dysfunction,
autophagy,
pro-death,
necrotic
apoptosis.
Therefore,
potential
a
therapeutic
target
management
promising.
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Март 25, 2025
Perforator
flap
transplantation
is
an
important
technique
in
reconstructive
surgery,
but
necrosis
limits
its
clinical
effectiveness.
Thymoquinone
(TQ),
a
natural
bioactive
plant
quinone
found
black
seed,
exhibits
anti-inflammatory,
angiogenic,
and
antimicrobial
properties.
This
study
investigates
the
therapeutic
effects
of
TQ
perforator
model
through
vivo
vitro
experiments.
Human
umbilical
vein
endothelial
cells
(HUVECs)
were
treated
with
Tert-butyl
Hydroperoxide
(TBHP)
to
simulate
then
TQ.
In
experiments
used
rat
model,
vascularization
was
assessed
using
Doppler
ultrasound
on
days
3
7
after
creation.
On
day
post-surgery,
samples
collected
evaluate
vascularity,
reactive
oxygen
species,
apoptosis
pyroptosis.
Network
pharmacology
analysis
conducted
identify
relevant
signaling
pathways,
molecular
docking
techniques
predict
potential
target
binding
sites.
results
showed
that
both
treatment
NLRP3
inhibitors
reduced
expression
pyroptosis-related
proteins.
indicated
TQ-treated
group
had
increased
survival
area,
blood
flow
intensity,
microvascular
density,
while
oxidative
stress,
apoptosis,
pyroptosis
levels
reduced.
Angiogenesis
enhanced,
SIRT1
protein
increased,
p-P65/NF-κB/NLRP3
pathway
downregulated.
After
inhibitor,
rate
angiogenesis
These
findings
suggest
improves
by
inhibiting
NF-κB/NLRP3
promoting
angiogenesis.
