Advanced NanoBiomed Research,
Год журнала:
2022,
Номер
3(2)
Опубликована: Дек. 23, 2022
Diabetic
wound,
also
known
as
diabetic
foot
ulcer
(DFU),
is
a
form
of
chronic
lesion
that
disrupts
the
patient's
lifestyle
and,
in
severe
cases,
may
be
fatal.
Recent
endeavors
utilizing
enzymes,
particularly
nanozymes
conjunction
with
other
materials,
have
revealed
potential
to
treat
wounds.
Herein,
rational
structural
design
enzyme‐based
biomaterials
summarized
after
presenting
types
and
processes
enzymes
employed
wound
healing,
goal
better
understanding
interaction
between
biological
materials.
The
importance
enzyme
catalytic
activity,
coupled
new
therapeutic
treatment
strategies,
emphasized
use
for
healing.
This
review
represents
most
recent
development
innovation
healing
applications,
it
can
used
reference
guide
regenerative
biomedicines
from
nanoenzyme
biomaterials.
ACS Applied Materials & Interfaces,
Год журнала:
2022,
Номер
14(18), С. 20641 - 20651
Опубликована: Апрель 28, 2022
Although
capsule-like
materials
as
host
carriers
for
enzyme
encapsulation
have
been
a
hot
topic
in
recent
years,
creating
an
ideal
microenvironment
enhanced
enzymatic
performance
is
still
formidable
challenge.
Herein,
we
created
template-free
method
to
situ
encapsulate
natural
enzymes
hollow
covalent
organic
framework
(COF)
capsules
at
room
temperature.
The
COF
crystallites
migrated
from
the
inner
core
and
self-assembled
outside
walls
during
inside-out
Ostwald
ripening
process,
retaining
cavity.
adjustable
structure
of
enzyme@COF
capsule
allowed
basic
vibration
maintain
certain
degree
freedom,
thus
significantly
enhancing
bioactivity.
has
large
mesoporous
tunnels
allowing
efficient
transport.
In
addition,
encapsulated
showed
superior
activity
ultrahigh
stability
under
various
extreme
conditions
that
may
lead
inactivation,
such
high
temperature,
solvents,
chelates,
denaturing
agent.
Finally,
prepared
GOx@COF
was
used
electrochemical
sensing
glucose
human
serum,
sensor
exhibited
selectivity
satisfactory
test
results.
This
research
not
only
provides
new
way
COFs
but
also
potential
applications
biocatalysis
biosensing,
making
artificial
organelles
possible.
Biosensors,
Год журнала:
2023,
Номер
13(6), С. 622 - 622
Опубликована: Июнь 5, 2023
Electrochemistry
is
a
genuinely
interdisciplinary
science
that
may
be
used
in
various
physical,
chemical,
and
biological
domains.
Moreover,
using
biosensors
to
quantify
or
biochemical
processes
critical
medical,
biological,
biotechnological
applications.
Nowadays,
there
are
several
electrochemical
for
healthcare
applications,
such
as
the
determination
of
glucose,
lactate,
catecholamines,
nucleic
acid,
uric
so
on.
Enzyme-based
analytical
techniques
rely
on
detecting
co-substrate
or,
more
precisely,
products
catalyzed
reaction.
The
glucose
oxidase
enzyme
generally
enzyme-based
measure
tears,
blood,
etc.
among
all
nanomaterials,
carbon-based
nanomaterials
have
been
utilized
thanks
unique
properties
carbon.
sensitivity
can
up
pM
levels
nanobiosensor,
these
sensors
very
selective,
enzymes
specific
their
substrates.
Furthermore,
frequently
fast
reaction
times,
allowing
real-time
monitoring
analyses.
These
biosensors,
however,
drawbacks.
Changes
temperature,
pH,
other
environmental
factors
influence
stability
activity
enzymes,
affecting
reliability
repeatability
readings.
Additionally,
cost
immobilization
onto
appropriate
transducer
surfaces
might
prohibitively
expensive,
impeding
large-scale
commercialization
widespread
use
biosensors.
This
review
discusses
design,
detection,
nanobiosensors,
recent
applications
studies
evaluated
tabulated.
Green Chemistry,
Год журнала:
2023,
Номер
25(12), С. 4591 - 4624
Опубликована: Янв. 1, 2023
This
review
details
the
potential
of
BDFMs
as
promising
carriers
for
enzymes
design
sustainable
and
robust
biocatalysts
with
improved
activity,
stability,
reusability.
Journal of Hazardous Materials,
Год журнала:
2023,
Номер
459, С. 132261 - 132261
Опубликована: Авг. 9, 2023
Efficient
enzyme
immobilization
is
crucial
for
the
successful
commercialization
of
large-scale
enzymatic
water
treatment.
However,
issues
such
as
lack
high
loading
coupled
with
leaching
present
challenges
widespread
adoption
immobilized
systems.
The
study
describes
development
and
bioremediation
application
an
biocomposite
employing
a
cationic
macrocycle-based
covalent
organic
framework
(COF)
hierarchical
porosity
horseradish
peroxidase
(HRP).
intrinsic
porous
features
azacalix[4]arene-based
COF
(ACA-COF)
allowed
maximum
HRP
capacity
0.76
mg/mg
low
(<5.0
%).
biocomposite,
HRP@ACA-COF,
exhibited
exceptional
thermal
stability
(∼200
%
higher
relative
activity
than
free
enzyme),
maintained
∼60
after
five
cycles.
LCMSMS
analyses
confirmed
that
HRP@ACA-COF
system
was
able
to
achieve
>
99
degradation
seven
diverse
types
emerging
pollutants
(2-mercaptobenzothiazole,
paracetamol,
caffeic
acid,
methylparaben,
furosemide,
sulfamethoxazole,
salicylic
acid)in
under
hour.
described
enzyme-COF
offers
promise
efficient
wastewater
applications.
