What Can an Organ-on-a-Chip Teach Us About Human Lung Pathophysiology? DOI
Haiqing Bai, Donald E. Ingber

Physiology, Год журнала: 2022, Номер 37(5), С. 242 - 252

Опубликована: Июнь 6, 2022

The intertwined relationship between structure and function has been key to understanding human organ physiology disease pathogenesis. An organ-on-a-chip (organ chip) is a bioengineered microfluidic cell culture device lined by living cells tissues that recapitulates organ-level functions in vitro. This accomplished recreating organ-specific tissue-tissue interfaces microenvironmental biochemical mechanical cues while providing dynamic perfusion through endothelium-lined vascular channels. In this review, we discuss how emerging technology contributed the of lung structure-function relationships at cell, tissue, levels.

Язык: Английский

Microfluidic Tissue Engineering and Bio‐Actuation DOI Creative Commons
Miriam Filippi, Thomas Büchner, Öncay Yaşa

и другие.

Advanced Materials, Год журнала: 2022, Номер 34(23)

Опубликована: Фев. 23, 2022

Abstract Bio‐hybrid technologies aim to replicate the unique capabilities of biological systems that could surpass advanced artificial technologies. Soft bio‐hybrid robots consist synthetic and living materials have potential self‐assemble, regenerate, work autonomously, interact safely with other species environment. Cells require a sufficient exchange nutrients gases, which is guaranteed by convection diffusive transport through liquid media. The functional development long‐term survival tissues in vitro can be improved dynamic flow culture, but only microfluidic control develop tissue fine structuring regulation at microscale. Full growth microscale will eventually lead macroscale constructs, are needed as component soft This review summarizes recent progress techniques engineer tissues, focusing on use muscle cells for robotic bio‐actuation. Moreover, instances bio‐actuation greatly benefit from fusion microfluidics highlighted, include: microfabrication matrices, biomimicry cell microenvironments, maturation, perfusion, vascularization.

Язык: Английский

Процитировано

67
Implementing organ-on-chip in a next-generation risk assessment of chemicals: a review DOI Creative Commons
Katharina S. Nitsche, Iris Müller,

Sophie Malcomber

и другие.

Archives of Toxicology, Год журнала: 2022, Номер 96(3), С. 711 - 741

Опубликована: Фев. 1, 2022

Organ-on-chip (OoC) technology is full of engineering and biological challenges, but it has the potential to revolutionize Next-Generation Risk Assessment novel ingredients for consumer products chemicals. A successful incorporation OoC into toolbox depends on robustness microfluidic devices organ tissue models used. Recent advances in standardized device manufacturing, cultivation growth protocols offer ability bridge gaps towards implementation organ-on-chip technology. an exposure-led hypothesis-driven tiered approach risk assessment using detailed human exposure information application appropriate new (non-animal) toxicological testing approaches. presents a promising vitro by combining cell culturing with dynamic microfluidics improve physiological emulation. Here, we critically review commercial devices, as well recent culture model studies skin, intestinal barrier liver main metabolic be used on-chip Assessment. Finally, microfluidically linked combinations such skin-liver intestine-liver are reviewed they form relevant aspect advancing toxicokinetic toxicodynamic studies. We point achievements challenges overcome, advance non-animal, human-relevant safety

Язык: Английский

Процитировано

46
Vat photopolymerization 3D printed microfluidic devices for organ-on-a-chip applications DOI
Laura A. Milton, Matthew Viglione, Louis Jun Ye Ong

и другие.

Lab on a Chip, Год журнала: 2023, Номер 23(16), С. 3537 - 3560

Опубликована: Янв. 1, 2023

Organs-on-a-chip, or OoCs, are microfluidic tissue culture devices with micro-scaled architectures that repeatedly achieve biomimicry of biological phenomena. They well positioned to become the primary pre-clinical testing modality as they possess high translational value. Current methods fabrication have facilitated development many custom OoCs generated promising results. However, reliance on microfabrication and soft lithographic techniques has limited their prototyping turnover rate scalability. Additive manufacturing, known commonly 3D printing, shows promise expedite this process, while also making easier more reproducible. We briefly introduce common printing modalities before identifying two sub-types vat photopolymerization - stereolithography (SLA) digital light processing (DLP) most advantageous for future OoC development. then outline motivations shifting requirements printed be competitive current state art, several considerations achieving successful touching design techniques, including a survey commercial printers resins. In all, we aim form guide end-user facilitate in-house generation along translation these important devices.

Язык: Английский

Процитировано

38
In vitro models to study human gut-microbiota interactions: Applications, advances, and limitations DOI Creative Commons

Yuli Qi,

Leilei Yu, Fengwei Tian

и другие.

Microbiological Research, Год журнала: 2023, Номер 270, С. 127336 - 127336

Опубликована: Фев. 16, 2023

Язык: Английский

Процитировано

33
Mechanopathology of biofilm-like Mycobacterium tuberculosis cords DOI Creative Commons
Richa Mishra, Mélanie T. M. Hannebelle, Vishal P. Patil

и другие.

Cell, Год журнала: 2023, Номер 186(23), С. 5135 - 5150.e28

Опубликована: Окт. 20, 2023

Mycobacterium tuberculosis (Mtb) cultured axenically without detergent forms biofilm-like cords, a clinical identifier of virulence. In lung-on-chip (LoC) and mouse models, cords in alveolar cells contribute to suppression innate immune signaling via nuclear compression. Thereafter, extracellular cause contact-dependent phagocyte death but grow intercellularly between epithelial cells. The absence these mechanopathological mechanisms explains the greater proportion lesions with increased infiltration dissemination defects cording-deficient Mtb infections. Compression lipid monolayers induces phase transition that enables mechanical energy storage. Agent-based simulations demonstrate storage capacity is sufficient for formation maintain structural integrity despite perturbation. Bacteria remain translationally active antibiotic exposure regrow rapidly upon cessation treatment. This study provides conceptual framework biophysics function infection therapy cord architectures independent ascribed single bacteria.

Язык: Английский

Процитировано

27
Recent Advances of Biosensor-Integrated Organ-on-a-Chip Technologies for Diagnostics and Therapeutics DOI
Ashwini Shinde, Kavitha Illath, Uvanesh Kasiviswanathan

и другие.

Analytical Chemistry, Год журнала: 2023, Номер 95(6), С. 3121 - 3146

Опубликована: Янв. 30, 2023

ADVERTISEMENT RETURN TO ISSUEReviewNEXTRecent Advances of Biosensor-Integrated Organ-on-a-Chip Technologies for Diagnostics and TherapeuticsAshwini ShindeAshwini ShindeDepartment Engineering Design, Indian Institute Technology Madras, Chennai 600036, IndiaMore by Ashwini ShindeView Biography, Kavitha IllathKavitha IllathDepartment IllathView Uvanesh KasiviswanathanUvanesh KasiviswanathanDepartment KasiviswanathanView Biographyhttps://orcid.org/0000-0003-3535-2244, Shalini NagabooshanamShalini NagabooshanamDepartment NagabooshanamView Biographyhttps://orcid.org/0000-0003-4856-6815, Pallavi GuptaPallavi GuptaDepartment GuptaView Koyel DeyKoyel DeyDepartment DeyView Pulasta ChakrabartyPulasta ChakrabartyDepartment ChakrabartyView Moeto NagaiMoeto NagaiDepartment Mechanical Engineering, Toyohashi University Technology, 441-8580, JapanMore NagaiView Suresh RaoSuresh RaoDepartment RaoView Srabani Kar*Srabani KarDepartment Physics, Science Education Research (IISER), Tirupati, Andhra Pradesh 517507, India*[email protected]More KarView Tuhin Subhra Santra*Tuhin SantraDepartment protected]; [email SantraView Biographyhttps://orcid.org/0000-0002-9403-2155Cite this: Anal. Chem. 2023, 95, 6, 3121–3146Publication Date (Web):January 30, 2023Publication History Received12 November 2022Published online30 January 2023Published inissue 14 February 2023https://pubs.acs.org/doi/10.1021/acs.analchem.2c05036https://doi.org/10.1021/acs.analchem.2c05036review-articleACS PublicationsCopyright © 2023 American Chemical SocietyRequest reuse permissionsArticle Views3446Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biotechnology,Cells,Electrical properties,Electrodes,Sensors Get e-Alerts

Язык: Английский

Процитировано

25
Gut-on-a-Chip Models: Current and Future Perspectives for Host–Microbial Interactions Research DOI Creative Commons
Moran Morelli, Dorota Kurek, Chee Ping Ng

и другие.

Biomedicines, Год журнала: 2023, Номер 11(2), С. 619 - 619

Опубликована: Фев. 18, 2023

The intestine contains the largest microbial community in human body, gut microbiome. Increasing evidence suggests that it plays a crucial role maintaining overall health. However, while many studies have found correlation between certain diseases and changes microbiome, impact of different compositions on mechanisms by which they contribute to disease are not well understood. Traditional pre-clinical models, such as cell culture or animal limited their ability mimic complexity physiology. New mechanistic organ-on-a-chip, being developed address this issue. These models provide more accurate representation physiology could help bridge gap clinical studies. Gut-on-chip allow researchers better understand underlying effect gut. They can move field from causation accelerate development new treatments for associated with This review will discuss current future perspectives gut-on-chip study host-microbial interactions.

Язык: Английский

Процитировано

25
Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery DOI Creative Commons

Qiaoxia Zhou,

Qiongliang Liu,

Yan Wang

и другие.

Advanced Science, Год журнала: 2024, Номер 11(14)

Опубликована: Янв. 28, 2024

Abstract Intracellular delivery of nano‐drug‐carriers (NDC) to specific cells, diseased regions, or solid tumors has entered the era precision medicine that requires systematic knowledge nano‐biological interactions from multidisciplinary perspectives. To this end, review first provides an overview membrane‐disruption methods such as electroporation, sonoporation, photoporation, microfluidic delivery, and microinjection with merits high‐throughput enhanced efficiency for in vitro NDC delivery. The impact characteristics including particle size, shape, charge, hydrophobicity, elasticity on cellular uptake are elaborated several types systems aiming hierarchical targeting vivo reviewed. Emerging ex human/animal‐derived pathophysiological models further explored highly recommended use studies since they might mimic features fill translational gaps animals humans. exploration modern microscopy techniques precise nanoparticle (NP) tracking at cellular, organ, organismal levels informs tailored development NDCs application clinical translation. Overall, integrates latest insights into smart nanosystem engineering, physiological models, imaging‐based validation tools, all directed towards enhancing efficient intracellular NDCs.

Язык: Английский

Процитировано

16
AI-Powered Microfluidics: Shaping the Future of Phenotypic Drug Discovery DOI
Junchi Liu, Hanze Du, Lei Huang

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(30), С. 38832 - 38851

Опубликована: Июль 17, 2024

Phenotypic drug discovery (PDD), which involves harnessing biological systems directly to uncover effective drugs, has undergone a resurgence in recent years. The rapid advancement of artificial intelligence (AI) over the past few years presents numerous opportunities for augmenting phenotypic screening on microfluidic platforms, leveraging its predictive capabilities, data analysis, efficient processing, etc. Microfluidics coupled with AI is poised revolutionize landscape discovery. By integrating advanced platforms algorithms, researchers can rapidly screen large libraries compounds, identify novel candidates, and elucidate complex pathways unprecedented speed efficiency. This review provides an overview advances challenges AI-based microfluidics their applications We discuss synergistic combination high-throughput AI-driven analysis phenotype characterization, drug-target interactions, modeling. In addition, we highlight potential AI-powered achieve automated system. Overall, represents promising approach shaping future by enabling rapid, cost-effective, accurate identification therapeutically relevant compounds.

Язык: Английский

Процитировано

13
An Organ‐on‐Chip Platform for Simulating Drug Metabolism Along the Gut–Liver Axis DOI Creative Commons
Mara Lucchetti, Kehinde Aina, Léa Grandmougin

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(20)

Опубликована: Март 7, 2024

The human microbiome significantly influences drug metabolism through the gut-liver axis, leading to modified responses and potential toxicity. Due complex nature of gut environment, understanding microbiome-driven impacts on these processes is limited. To address this, a multiorgan-on-a-chip (MOoC) platform that combines microbial-crosstalk (HuMiX) gut-on-chip (GoC) Dynamic42 liver-on-chip (LoC), mimicking bidirectional interconnection between liver known as introduced. This supports viability functionality intestinal cells. In proof-of-concept study, irinotecan, widely used colorectal cancer drug, imitated within MOoC. Utilizing liquid chromatography coupled tandem mass spectrometry (LC-MS/MS), irinotecan metabolites are tracked, confirming platform's ability represent along axis. Further, using authors' platform, it shown cancer-associated bacterium, Escherichia coli, modifies transformation its inactive metabolite SN-38G into toxic SN-38. serves robust tool for investigating intricate interplay microbes pharmaceuticals, offering representative alternative animal models providing novel development strategies.

Язык: Английский

Процитировано

11