Piezo1 Response to Shear Stress Is Controlled by the Components of the Extracellular Matrix

ACS Applied Materials & Interfaces, Год журнала: 2022, Номер 14(36), С. 40559 - 40568

Опубликована: Сен. 1, 2022

Piezo1 is a recently discovered Ca2+ permeable ion channel that has emerged as an integral sensor of hemodynamic forces within the cardiovascular system, contributing to vascular development and blood pressure regulation. However, how composition extracellular matrix (ECM) affects mechanosensitivity in response remains poorly understood. Using combination microfluidics calcium imaging techniques, we probe shear stress sensitivity single HEK293T cells engineered stably express presence different ECM proteins. Our experiments show not dependent on proteins regulate depending level. Under high stress, fibronectin sensitizes shear, while under low improved collagen types I IV laminin. Moreover, report α5β1 αvβ3 integrins are involved at αvβ5 regulating stress. These results demonstrate ECM/integrin interactions influence could represent mechanism whereby transmitted channels, providing new insights into by which senses

Язык: Английский

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Micropumps and Microvalves for Biomedical Applications

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118236 - 118236

Опубликована: Март 1, 2025

Язык: Английский

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Emerging microfluidics for bioreactor

Materials Today, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Biophysical assays to test cellular mechanosensing: moving towards high throughput

Biophysical Reviews, Год журнала: 2024, Номер 16(6), С. 875 - 882

Опубликована: Дек. 1, 2024

Mechanosensitivity is the ability of cells to sense and respond mechanical stimuli. In order do this, are endowed with different components that allow them react a broad range stimuli, such as compression or shear forces, pressure, vibrations. This sensing process, mechanosensing, involved in fundamental physiological mechanisms, stem cell differentiation migration, but it also central development pathogenic states. Here, we review approaches have been proposed quantify mechanosensation living cells, specific focus on methodologies enable higher experimental throughput. aspect crucial fully understand nuances how impacts physiology pathology systems. We will discuss traditional methods for studying mechanosensing at level single particular attention activation mechanosensitive ion channel piezo1. Moreover, present recent attempts push analysis towards

Язык: Английский

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Quantitative mechanical stimulation of GPR68 using a novel 96 well flow plugin

Lab on a Chip, Год журнала: 2024, Номер 24(6), С. 1616 - 1625

Опубликована: Янв. 1, 2024

Mechanosensitive proteins play a crucial role in range of physiological processes, including hearing and regulating blood flow. This work presents novel microfluidic approach compatible with 96-well plates to quantify their mechanosensitivity.

Язык: Английский

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Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Abstract Mechanotransduction is the process whereby cells convert mechanical signals into electrochemical responses, where mechanosensitive proteins mediate this interaction. To characterize these critical proteins, numerous techniques have been developed that apply forces and measure subsequent cellular responses. While approaches given insight specific aspects of many such validation cross‐comparison between remain difficult significant variations in reported activation thresholds responses for same protein across different studies. Accurately determining mechanosensitivity various however, essential understanding mechanotransduction potential physiological implications, including therapeutics. This review provides an assessment current emerging used ion channel G‐Coupled Receptors (GPCRs) stimulation measurement, with a focus on ability to quantitatively

Язык: Английский

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Functional Role of Piezo1 in the Human Eosinophil Cell Line AML14.3D10: Implications for the Immune and Sensory Nervous Systems

Biomolecules, Год журнала: 2024, Номер 14(9), С. 1157 - 1157

Опубликована: Сен. 14, 2024

Mechanosensitive ion channels, particularly Piezo are widely expressed in various tissues. However, their role immune cells remains underexplored. Therefore, this study aimed to investigate the functional of Piezo1 human eosinophil cell line AML14.3D10. We detected

Язык: Английский

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