A Serum Metabolomics Study Based on LC-MS: Chemosensitization Effects of Rauvolfia Vomitoria Afzel. Combined with 5-Fluorouracil on Colorectal Cancer Mice DOI
Zhifan Wang,

Wen-Ru Kong,

Ning Wang

и другие.

SSRN Electronic Journal, Год журнала: 2022, Номер unknown

Опубликована: Янв. 1, 2022

Colorectal cancer (CRC) is one of the malignant tumors with high incidence, and mainly treated by chemotherapy at present. However, during CRC treatment, long-term use traditional chemotherapeutic drugs will reduce sensitivity chemotherapy. Our previous studies have shown that Rauvolfia vomitoria total alkaloids (RVA) played an important role in 5-fluorouracil (5-FU)chemosensitization therapy, but its intervention mechanism has not been clarified completely metabolic level. Therefore, this study, LC-MS based metabolomics was employed to explore 5-FU chemosensitization induced combination RVA conventional 5-FUTo evaluate effects RVA, serum samples were collected from control group, treatment low-dose high-dose combined group respectively. Serum analyzed HPLC-Q-Exactive Orbitrap/MS, metabolites identified HMDB database. After multivariate statistical analysis identification, 33 differential between among which glycerophospholipid pathway pentose glucuronate interconversions mostly affected. These results suggest may sensitize drug exert anti-tumor activity through influencing glycerophosphplipid metabolism cell energy metabolism. Metabolomics provided a new insight into estimate therapeutic effect dissection potential mechanisms Chinese medicine treating colorectal cancer.

Язык: Английский

A Serum Metabolomics Study Based on LC-MS: Chemosensitization Effects of Rauvolfia Vomitoria Afzel. Combined with 5-Fluorouracil on Colorectal Cancer Mice DOI
Zhifan Wang,

Wen-Ru Kong,

Ning Wang

и другие.

SSRN Electronic Journal, Год журнала: 2022, Номер unknown

Опубликована: Янв. 1, 2022

Colorectal cancer (CRC) is one of the malignant tumors with high incidence, and mainly treated by chemotherapy at present. However, during CRC treatment, long-term use traditional chemotherapeutic drugs will reduce sensitivity chemotherapy. Our previous studies have shown that Rauvolfia vomitoria total alkaloids (RVA) played an important role in 5-fluorouracil (5-FU)chemosensitization therapy, but its intervention mechanism has not been clarified completely metabolic level. Therefore, this study, LC-MS based metabolomics was employed to explore 5-FU chemosensitization induced combination RVA conventional 5-FUTo evaluate effects RVA, serum samples were collected from control group, treatment low-dose high-dose combined group respectively. Serum analyzed HPLC-Q-Exactive Orbitrap/MS, metabolites identified HMDB database. After multivariate statistical analysis identification, 33 differential between among which glycerophospholipid pathway pentose glucuronate interconversions mostly affected. These results suggest may sensitize drug exert anti-tumor activity through influencing glycerophosphplipid metabolism cell energy metabolism. Metabolomics provided a new insight into estimate therapeutic effect dissection potential mechanisms Chinese medicine treating colorectal cancer.

Язык: Английский

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