EES Catalysis, Год журнала: 2023, Номер 2(1), С. 14 - 48
Опубликована: Окт. 28, 2023
This article reviews the integration of multidisciplinary approaches, including protein engineering, computational biology, and nanoarchitectonics, to advance pharmaceutical enzyme biocatalysis.
Язык: Английский
Natural Products and Bioprospecting, Год журнала: 2024, Номер 14(1)
Опубликована: Янв. 11, 2024
Abstract Metagenomics has opened new avenues for exploring the genetic potential of uncultured microorganisms, which may serve as promising sources enzymes and natural products industrial applications. Identifying with improved catalytic properties from vast amount available metagenomic data poses a significant challenge that demands development novel computational functional screening tools. The all are primarily dictated by their structures, predominantly determined amino acid sequences. However, this aspect not been fully considered in enzyme bioprospecting processes. With accumulating number sequences increasing demand discovering biocatalysts, structural modeling can be employed to identify properties. Recent efforts discover polysaccharide-degrading rumen metagenome using homology-based searches machine learning-based models have shown promise. Here, we will explore various approaches screen shortlist metagenome-derived biocatalyst candidates, conjunction wet lab analytical methods traditionally used characterization.
Язык: Английский
Процитировано
15
Process Biochemistry, Год журнала: 2024, Номер 143, С. 323 - 336
Опубликована: Май 3, 2024
Язык: Английский
Процитировано
9
Advanced Drug Delivery Reviews, Год журнала: 2022, Номер 183, С. 114143 - 114143
Опубликована: Фев. 12, 2022
Язык: Английский
Процитировано
35
Current Opinion in Microbiology, Год журнала: 2022, Номер 67, С. 102142 - 102142
Опубликована: Март 5, 2022
Язык: Английский
Процитировано
35
The Journal of Microbiology, Год журнала: 2022, Номер 60(10), С. 969 - 976
Опубликована: Сен. 27, 2022
Язык: Английский
Процитировано
34
Nature Chemical Biology, Год журнала: 2022, Номер 18(10), С. 1096 - 1103
Опубликована: Июль 7, 2022
Язык: Английский
Процитировано
30
International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(8), С. 7334 - 7334
Опубликована: Апрель 15, 2023
Epoxide hydrolases are attractive and industrially important biocatalysts. They can catalyze the enantioselective hydrolysis of epoxides to corresponding diols as chiral building blocks for bioactive compounds drugs. In this review article, we discuss state art development potential epoxide biocatalysts based on most recent approaches techniques. The covers new discover using genome mining enzyme metagenomics, well improving activity, enantioselectivity, enantioconvergence, thermostability by directed evolution a rational design. Further improvements in operational storage stabilization, reusability, pH thermal stabilization immobilization techniques discussed study. New possibilities expanding synthetic capabilities their involvement non-natural cascade reactions described.
Язык: Английский
Процитировано
21
Catalysts, Год журнала: 2023, Номер 13(6), С. 961 - 961
Опубликована: Июнь 1, 2023
Biocatalysis is currently a workhorse used to produce wide array of compounds, from bulk fine chemicals, in green and sustainable manner. The success biocatalysis largely thanks an enlargement the feasible chemical reaction toolbox. This materialized due major advances enzyme screening tools methods, together with high-throughput laboratory techniques for biocatalyst optimization through engineering. Therefore, enzyme-related knowledge has significantly increased. To handle large number data now available, computational approaches have been gaining relevance biocatalysis, among them machine learning methods (MLMs). MLMs use algorithms learn improve experience automatically. review intends briefly highlight contribution within biochemical engineering bioprocesses present key aspects scope related fields, mostly readers non-skilled mind. Accordingly, brief overview basic concepts underlying are presented. complemented steps build model followed by insights into types intelligently analyse data, identify patterns develop realistic applications bioprocesses. Notwithstanding, given this review, some recent illustrative examples protein engineering, production, formulation provided, future developments suggested. Overall, it envisaged that will provide how these assets more efficient biocatalysis.
Язык: Английский
Процитировано
17
Biocatalysis and Agricultural Biotechnology, Год журнала: 2024, Номер 56, С. 103042 - 103042
Опубликована: Янв. 26, 2024
Today, almost all human health problems are related to malnutrition. Algae (macro- and micro-algae) potent provide the necessary nutrients for our bodies (i.e., starch, lipids, protein, dietary fiber, minerals, vitamins). Nevertheless, there limitations due extraction efficiency, size, compatibility, hydrocolloid nature of some algal compounds interest food industry. As a result, enzyme-assisted biomass under optimal conditions will be safer more sustainable approach than using hazardous organic solvents. Also, enzymes can used modify molecular structures introduce new biomolecules that exhibit higher stability industries. Throughout this review, we explore most recent research on process use as food.
Язык: Английский
Процитировано
6
Environmental Pollution, Год журнала: 2024, Номер 346, С. 123572 - 123572
Опубликована: Фев. 16, 2024
Язык: Английский
Процитировано
6