RNA interference cannot be operated in lepidopteran insect? A nanocarrier breaks bottlenecks at all developmental stages of Spodoptera frugiperda DOI Creative Commons

Zijian Chao,

Zhongzheng Ma,

Yunhui Zhang

и другие.

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Окт. 12, 2022

Abstract RNA interference (RNAi) has become an essential technique in functional analysis of insect genes and pest management. However, insensitivity to RNAi is the main obstacle that limits identification lepidopteran insects. Hereby we explored efficient nanocarrier (star polycation, SPc)-mediated dsRNA delivery system achieve at all developmental stages Spodoptera frugiperda . Double-stranded (dsRNA) was synthesized targeting a consistently expressed gene V-type proton ATPase subunit d ( ATP-d ) as well enhanced green fluorescent protein eGFP for various operation tests. At egg early larval stages, SPc-loaded could penetrate physical obstacles eggshell body wall. Thus, soaking topical applications were suitable 1 st -2 nd instar respectively. The SPc protect from degradation by midgut fluid mid-late larvae. Thereby oral feeding silenced target more efficiently compared alone. Finally, pupal adult injection enough induce high effect. A key gene, Chitin deacetylase CDA1 ), taken example test control efficiency SPc-based system. constructed remarkably knockdown lead growth retardation mortality. Overall, present study nanocarrier-based whole insects, which would provide powerful tool identifying

Язык: Английский

Nano-enabled weed management in agriculture: From strategic design to enhanced herbicidal activity DOI Creative Commons
Mariana Monteiro de Lima Forini, Montcharles da Silva Pontes,

Débora R. Antunes

и другие.

Plant Nano Biology, Год журнала: 2022, Номер 1, С. 100008 - 100008

Опубликована: Июнь 1, 2022

The use of nanotechnology has emerged in the agricultural sector. In recent years, several nano-enabled materials have been reported for weed control. Nano-enabled herbicides (also called nanoherbicides) can be designed from organic, inorganic, or hybrid materials. These unique properties such as small size, specific surface area, and ability to control release metal ions organic molecules field. Some studies nanoherbicides provide better management compared non-nanoformulations. However, regarding environmental risk assessment mechanisms action plants are still incipient. Nevertheless, they essential ensure a safe application human health environment. this review, we outlined current understanding well some strategic design fabricate nanostructures Also, critical discussion fate, behavior effects was addressed order achieve products environmentally friendly sustainable agriculture.

Язык: Английский

Процитировано

46
RNAi turns 25:contributions and challenges in insect science DOI Creative Commons
Subba Reddy Palli

Frontiers in Insect Science, Год журнала: 2023, Номер 3

Опубликована: Окт. 4, 2023

Since its discovery in 1998, RNA interference (RNAi), a Nobel prize-winning technology, made significant contributions to advances biology because of ability mediate the knockdown specific target genes. RNAi applications medicine and agriculture have been explored with mixed success. The past 25 years research on resulted our understanding mechanisms action, specificity, differential efficiency among animals plants. played major role insect biology. Did technology fully meet pest disease vector management expectations? This review will discuss recent science. remaining challenges, including delivery site, efficiency, potential resistance development possible solutions for widespread use this management.

Язык: Английский

Процитировано

35
Advances in nanocarriers to improve the stability of dsRNA in the environment DOI Creative Commons
Wenling Yang, Baitao Wang, Lei Gao

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Авг. 16, 2022

RNAi technology, known as a revolutionary technology in the history of pesticides, has been identified very promising novel approach for crop protection, which is great significance achieving sustainable agricultural development United Nations Food and Agriculture Organization. Although many studies have shown that RNA biopesticides strong application prospects, its stability seriously restricts commercial use. As core component RNAi, double-stranded (dsRNA) unstable natural form. Therefore, how to ensure dsRNA one most significant challenges realizing use biopesticides. Nanomaterials such cationic polymers lipofectamine can improve environment, proved. This paper reviews recent research progress nanomaterials be used environmental dsRNA, discusses advantages limitations different combined with provides reference selection nanoformulations.

Язык: Английский

Процитировано

39
An effective fluorescence sensor for herbicide haloxyfop-P-methyl based on cyclic bicyanostilbene imidazole salt DOI

Yunmei Wu,

Pengpeng Wang,

Linlu Zheng

и другие.

Sensors and Actuators B Chemical, Год журнала: 2023, Номер 380, С. 133330 - 133330

Опубликована: Янв. 10, 2023

Язык: Английский

Процитировано

23
A Star Polyamine-Based Nanocarrier Delivery System for Enhanced Avermectin Contact and Stomach Toxicity against Green Peach Aphids DOI Creative Commons
Yanxiao Yang, Qinhong Jiang,

Min Peng

и другие.

Nanomaterials, Год журнала: 2022, Номер 12(9), С. 1445 - 1445

Опубликована: Апрель 23, 2022

The unscientific application of synthesized/botanical pesticides has not only brought the resistance plant pathogens and pests, but also led to serious environmental pollution. In recent years, various nano-delivery systems have been used for development environmental-friendly with improved efficacy. Herein, current study constructed an efficient avermectin B1a (AVM) system based on a star polyamine (SPc) focused characterization bioactivity SPc-loaded AVM at mass ratios. hydroxyl groups could assemble carbonyl SPc through hydrogen bond van der Waals forces, self-assembly formed nearly spherical particles AVM/SPc complex nanoscale size. contact angle decreased increasing ratio SPc, revealing easier distribution spreading complex. Furthermore, stomach toxicity increased along ratio, which be attributed enhanced systemic transportation in plants, enlarged area insect pests stronger permeability across cuticle. provides wide applications agricultural field.

Язык: Английский

Процитировано

28
Synthetic Nanoscale RNAi Constructs as Pesticides for the Control of Locust Migratoria DOI
Qiong Lu, Hongyan Cui, Wenda Li

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2022, Номер 70(35), С. 10762 - 10770

Опубликована: Авг. 24, 2022

The low efficiency of RNA interference (RNAi) in insects via the oral administration double-stranded (dsRNA) is a considerable obstacle preventing its application insect pest control. instability dsRNA and insufficient uptake are known to limit RNAi efficiency. To overcome these limitations, block copolymer poly(ethylene glycol)-polylysine(thiol) [PEG-PLys(SH)] was designed this study form well-defined, core–shell nanoparticles protect from premature degradation facilitate movement through various physiological barriers. developed material had excellent structural stability dsRNA-protecting capacity, thereby enabling prolonged survival digestive tract for endocytosis into midgut cells migratory locust, Locusta migratoria. After encapsulation dsLmCHS2 payload (a gene), 60% down-regulation LmCHS2, accompanied with observations amorphous discontinuous linings peritrophic matrix abnormal phenotypes, observed. In addition, elaborated nanoscale condensates appeared readily extravasate narrow fenestrations epithelial hemolymph be distributed throughout body. dsLmCHS1 cuticle distinctive lethal phenotype molting failure observed as result 50% LmCHS1. persistent leaf adherence constructs also capable resisting continuous rinsing. Therefore, represent robust type pesticide, which has potential versatile pesticide against variety molecular targets control destructive resistant conventional pesticides.

Язык: Английский

Процитировано

27
Chronic exposure to the star polycation (SPc) nanocarrier in the larval stage adversely impairs life history traits in Drosophila melanogaster DOI Creative Commons
Shuo Yan, Na Li,

Yuankang Guo

и другие.

Journal of Nanobiotechnology, Год журнала: 2022, Номер 20(1)

Опубликована: Дек. 8, 2022

Abstract Background Nanomaterials are widely used as pesticide adjuvants to increase efficiency and minimize environmental pollution. But it is increasingly recognized that nanocarrier a double-edged sword, nanoparticles emerging new pollutants. This study aimed determine the biotoxicity of applied star polycation (SPc) using Drosophila melanogaster , fruit fly, an in vivo model. Results The lethal concentration 50 (LC ) value SPc was identified 2.14 g/L toward third-instar larvae 26.33 for adults. Chronic exposure sub (1 g/L) larval stage showed long-lasting adverse effects on key life history traits. Exposure at adversely impacted lifespan, fertility, climbing ability well stresses resistance emerged RNA-sequencing analysis found resulted aberrant expression genes involved metabolism, innate immunity, stress response hormone production larvae. Orally administrated were mainly accumulated intestine cells, while systemic responses observed. Conclusions These findings indicate hazardous flies multiple levels, which could help us develop guidelines further large-scale application.

Язык: Английский

Процитировано

26
A spray-induced gene silencing strategy for Spodoptera frugiperda oviposition inhibition using nanomaterial-encapsulated dsEcR DOI
Ningning Li,

Xiaona Xu,

Jiwen Li

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 281, С. 136503 - 136503

Опубликована: Окт. 11, 2024

Язык: Английский

Процитировано

4
Complicated gene network for regulating feeding behavior: novel efficient target for pest management DOI Open Access

Jiajia Zhao,

Jiaming Yin,

Wang Zeng

и другие.

Pest Management Science, Год журнала: 2024, Номер unknown

Опубликована: Окт. 10, 2024

Abstract Feeding behavior is a fundamental activity for insects, which essential their growth, development and reproduction. The regulation of feeding complicated process influenced by variety factors, including external stimuli internal physiological signals. current review introduces the signaling pathways in brain, gut fat body involved insect behavior, provides series target genes developing RNA pesticides. Additionally, this summaries challenges identification application functional finally proposes future research direction. © 2024 Society Chemical Industry.

Язык: Английский

Процитировано

3
Notch Signaling in Insect Development: A Simple Pathway with Diverse Functions DOI Open Access
Yao Chen, Haomiao Li, T. Yi

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(18), С. 14028 - 14028

Опубликована: Сен. 13, 2023

Notch signaling is an evolutionarily conserved pathway which functions between adjacent cells to establish their distinct identities. Despite operating in a simple mechanism, plays remarkably diverse roles development regulate cell fate determination, organ growth and tissue patterning. While initially discovered characterized the model insect Drosophila melanogaster, recent studies across various species have revealed broad involvement of shaping tissues. This review focuses on providing comprehensive picture regarding development. The formation patterning embryo, wing, leg, ovary several specific structures, as well physiological responses, are summarized. These results discussed within developmental context, aiming deepen our understanding diversified different species.

Язык: Английский

Процитировано

8