Revealing the anti-senescence effects and related mechanisms of flavonoid extracts from the buds of Wikstroemia chamaedaphne Meisn on D-galactose-induced PC12 cells based on network pharmacology and transcriptomics

Future Journal of Pharmaceutical Sciences, Год журнала: 2025, Номер 11(1)

Опубликована: Март 10, 2025

Abstract Background Natural products are an important source of drugs or lead compounds for the treatment senescence. The buds Wikstroemia chamaedaphne Meisn a traditional Chinese medicine to cure edema, schizophrenia and epilepsy. A flavonoid extract W. (FEW) was prepared from methanolic by our group previously, which including eight flavonoids with content (55.19 ± 0.32) %. In this study, anti-senescence effects related mechanisms FEW on D-galactose-induced PC12 cells were investigated first time. Results High doses D-galactose could induce cell senescence, whereas delay senescence decreasing SA-β-gal positivity, increasing SOD activity, reducing MDA levels, improving morphology, inhibiting cycle arrest down-regulating expression senescence-related proteins P16, P21 P53. Subsequently, potential underlying elucidated through integration network pharmacology transcriptomics. main signaling pathways involved found be cancer pathway, FOXO PI3k–Akt AGE–RAGE protein digestion uptake, etc. may pathway as revealed western blot experiments. Conclusion Our study that has effects. This suggest acts agent age-related neurological diseases.

Язык: Английский

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Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(19), С. 11220 - 11220

Опубликована: Сен. 23, 2022

Polysaccharide from Polygonatum sibiricum (PSP) possesses antioxidant, antiaging, and neuroprotective activities. However, whether how the steaming process influences biological activities of PSP, especially against aging-related memory impairment, is not yet known. In this study, rhizome was subjected to a "nine nine drying" process, then PSPs with different times were abstracted. Thereafter, physicochemical properties qualified; antioxidant evaluated in D-gal-induced HT-22 cell model, effects (PSP0, PSP5 PSP9) on using D-gal-injured mice. Our results showed that while steamed had low pH value large negative charge, they shared similar main chains substituents. Cellular experiments activity increased. PSP0, PSP5, PSP9 could significantly ameliorate impairment mice, showing optimal effect. Meanwhile, demonstrated best effect terms preventing death synaptic injury Additionally, increased anti-oxidative stress-related protein expression decreased inflammation-related Collectively, improves likely by increasing PSPs.

Язык: Английский

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Bioactive Peptides from Edible Mushrooms—The Preparation, Mechanisms, Structure—Activity Relationships and Prospects

Foods, Год журнала: 2023, Номер 12(15), С. 2935 - 2935

Опубликована: Авг. 2, 2023

Mushroom bioactive peptides (MBPs) are extracted directly or indirectly from edible mushrooms. MBPs known to have antioxidant, anti-aging, antibacterial, anti-inflammatory and anti-hypertensive properties, facilitate memory cognitive improvement, antitumour anti-diabetes activities, cholesterol reduction. exert antioxidant effects by regulating the MAPK, Keap1-Nrf2-ARE, NF-κB TNF pathways. In addition, anti-tumour stimulating proliferation of macrophages. The bioactivities closely related their molecular weights, charge, amino acid compositions sequences. Compared with animal-derived peptides, ideal raw materials for healthy functional products advantages abundance resources, safety, low price, easy-to-achieve large-scale production valuable nutrients health maintenance disease prevention. this review, preparation, bioactivities, mechanisms structure–activity relationships were described. main challenges prospects application in also discussed. This review aimed provide a comprehensive perspective MBPs.

Язык: Английский

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Methylated urolithin A, mitigates cognitive impairment by inhibiting NLRP3 inflammasome and ameliorating mitochondrial dysfunction in aging mice

Neuropharmacology, Год журнала: 2024, Номер 252, С. 109950 - 109950

Опубликована: Апрель 16, 2024

Язык: Английский

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Bioactive Peptides and Other Immunomodulators of Mushroom Origin

Biomedicines, Год журнала: 2024, Номер 12(7), С. 1483 - 1483

Опубликована: Июль 4, 2024

For centuries, humans have used mushrooms as both food and pro-health supplements. Mushrooms, especially those related to the functions of human immune system, are rich in dietary fiber, minerals, essential amino acids, various bioactive compounds significant health-promoting properties. Immunoregulatory include lectins, terpenes, terpenoids, polysaccharides, fungal immunomodulatory proteins (FIPs). The distribution these varies from one species mushroom another, their activities depend on core structures chemical modifications composition fractions. In this review, we describe active medical mushrooms. We summarize potential mechanisms for vitro vivo detail approaches developing applying Finally, discuss applications peptides highlight areas that require improvement before widespread use therapeutic agents explore status clinical studies products, well prospect application AMPs ‘drug-like’ with great treatment non-healing chronic wounds multiresistant infections.

Язык: Английский

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PTP1B knockdown alleviates BMSCs senescence via activating AMPK-mediated mitophagy and promotes osteogenesis in senile osteoporosis

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2023, Номер 1869(7), С. 166795 - 166795

Опубликована: Июнь 28, 2023

Язык: Английский

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Icariin Targets p53 to Protect Against Ceramide-Induced Neuronal Senescence: implication in Alzheimer’s Disease

Free Radical Biology and Medicine, Год журнала: 2024, Номер 224, С. 204 - 219

Опубликована: Авг. 26, 2024

Язык: Английский

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γ‐glutamylcysteine alleviates insulin resistance and hepatic steatosis by regulating adenylate cyclase and IGF‐1R/IRS1/PI3K/Akt signaling pathways

The Journal of Nutritional Biochemistry, Год журнала: 2023, Номер 119, С. 109404 - 109404

Опубликована: Июнь 11, 2023

Язык: Английский

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γ-Glutamylcysteine ameliorates blood-brain barrier permeability and neutrophil extracellular traps formation after ischemic stroke by modulating Wnt/β-catenin signalling in mice

European Journal of Pharmacology, Год журнала: 2024, Номер 969, С. 176409 - 176409

Опубликована: Фев. 15, 2024

Язык: Английский

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γ-Glutamylcysteine suppresses epithelial-mesenchymal transition in lens epithelial cells to ameliorate diabetic cataract through regulating TGF-β2 and AMPK-related signaling pathways

Journal of Functional Foods, Год журнала: 2025, Номер 127, С. 106725 - 106725

Опубликована: Март 14, 2025

Язык: Английский

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