The influence of biophysical niche on tumor-associated macrophages in liver cancer DOI Creative Commons
Ying Zhang,

Ying Rao,

Jiahuan Lu

и другие.

Hepatology Communications, Год журнала: 2024, Номер 8(11)

Опубликована: Окт. 29, 2024

HCC, the most common type of primary liver cancer, is a leading cause cancer-related mortality worldwide. Although advancement immunotherapies by immune checkpoint inhibitors (ICIs) that target programmed cell death 1 or 1-ligand has revolutionized treatment for majority still not beneficial. Accumulating evidence pointed out potent immunosuppressive tumor microenvironment in HCC poses great challenge to ICI therapeutic efficacy. As key component microenvironment, tumor-associated macrophages (TAMs) play vital roles development, progression, and low responsiveness. Mechanistically, TAM can promote cancer invasion metastasis, angiogenesis, epithelial-mesenchymal transition, maintenance stemness, importantly, immunosuppression. Targeting TAMs, therefore, represents an opportunity enhance efficacy patients with HCC. While previous research primarily focused on biochemical cues influencing macrophages, emerging highlights critical role biophysical signals, such as substrate stiffness, topography, external forces. In this review, we summarize influence characteristics within regulate phenotype function TAMs pathogenesis progression. We also explore possible mechanisms discuss potential manipulating regulating therapy. By gaining deeper understanding how sense respond mechanical forces, may potentially usher path toward curative approach combinatory immunotherapies.

Язык: Английский

Substrate curvature influences cytoskeletal rearrangement and modulates macrophage phenotype DOI Creative Commons

Austin Sovar,

Matthew D. Patrick, Ramkumar T. Annamalai

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 6, 2025

Inflammation is a vital immune response, tightly orchestrated through both biochemical and biophysical cues. Dysregulated inflammation contributes to chronic diseases, highlighting the need for novel therapies that modulate responses with minimal side effects. While several pathways of are well understood, influence physical properties such as substrate curvature on cell behavior remains underexplored. This study investigates how impacts macrophage cytoskeletal dynamics, gene expression, immunophenotype mechanosensitive pathways. Gelatin-based microgels tunable surface curvatures were fabricated via water-in-oil emulsification crosslinked genipin. Microgels sorted into three size ranges, yielding high (40-50 µm), intermediate (150-250 low (350-400 µm) profiles. Macrophages seeded onto these microgels, dynamics examined using confocal microscopy, SEM, actin-specific staining. Gene expression pro- anti-inflammatory markers was quantified qPCR. The role actin polymerization assessed Latrunculin-A (Lat-A) treatment. adhered effectively high- low-curvature displaying curvature-dependent morphological changes. Confocal imaging revealed macrophages exhibited significantly higher F-actin density than those high-curvature microgels. Correspondingly, qPCR analysis showed upregulation pro-inflammatory (e.g., Tnf, Nos2) in conditions, while Arg1) elevated conditions. Lat-A treatment reduced modulated patterns, confirming regulation phenotype. These findings demonstrate influences by modulating associated immunophenotypic actin-mediated transcriptional By controlling curvature, therapeutic biomaterials may direct responses, offering new avenue treating inflammatory diseases. mechanobiological approach presents promising strategy precision immunomodulation regenerative medicine.

Язык: Английский

Процитировано

1
Tailoring surface stiffness to modulate senescent macrophage immunomodulation: Implications for osteo-/angio-genesis in aged bone regeneration DOI
Yi Zhang,

Jinjun Dai,

Ruiyue Hang

и другие.

Biomaterials Advances, Год журнала: 2024, Номер 165, С. 214010 - 214010

Опубликована: Авг. 28, 2024

Язык: Английский

Процитировано

6
A crosstalk between adhesion and phagocytosis integrates macrophage functions into their microenvironment DOI Creative Commons
Manon Depierre, Anna Mularski, Artur Ruppel

и другие.

iScience, Год журнала: 2025, Номер unknown, С. 112067 - 112067

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Matrix Viscoelasticity Orchestrates Osteogenesis via Mechanotransduction Mediated Metabolic Switch in Macrophages DOI Creative Commons

Dihao Tao,

Hanzhe Wang,

Shiping Chang

и другие.

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Understanding the interplay between extracellular matrix (ECM) mechanics and macrophage cellular processes is crucial for bone regeneration. While ECM stiffness has been extensively studied, role of viscoelasticity (e.g., stress relaxation) in marrow niche its effects on function remain unclear. Here, this study reveals how orchestrates osteogenesis by modulating metabolism through vasodilator-stimulated phosphoprotein (VASP) / hypoxia-inducible factor 1 alpha (HIF1α) signaling. In rapid maxillary expansion (RME) model, significant relaxation occurs regenerated during initial 17 days, coinciding with increased transforming growth factor-beta (TGF-β1+) F4/80+ macrophages. Fast enhances recruitment mesenchymal stem cells (MSCs) upregulating TGF-β1. Using a hydrogel-macrophage system mimicking viscoelasticity, cranial defect regeneration significantly improved. Moreover, fast shifts from glycolysis to oxidative phosphorylation (OXPHOS) via VASP/HIF1α signaling, facilitating reparative phenotype. These findings elucidate relationship metabolism, suggesting new therapeutic avenues mechanomedicine.

Язык: Английский

Процитировано

0
CaMKK2 Regulates Macrophage Polarization Induced by Matrix Stiffness: Implications for Shaping the Immune Response in Stiffened Tissues DOI Creative Commons
Ya Guan, Min Zhang, Jiyeon Song

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Macrophages are essential for immune responses and maintaining tissue homeostasis, exhibiting a wide range of phenotypes depending on their microenvironment. The extracellular matrix (ECM) is vital component that provides structural support organization to tissues, with stiffness acting as key regulator macrophage behavior. Using physiologically relevant 3D stiffening hydrogel models, it found increased alone promoted polarization toward pro-regenerative phenotype, mimicking the effect interleukin-4(IL-4) in softer matrices. Blocking Calcium/calmodulin-dependent kinase 2 (CaMKK2) selectively inhibited stiffness-induced without affecting IL-4-driven pathways. In functional studies, CaMKK2 deletion prevented M2-like/pro-tumoral caused by stiffening, which turn hindered tumor growth. murine wound healing model, loss impaired stiffness-mediated accumulation, ultimately disrupting vascularization. These findings highlight critical role mechanosensitive fate determination gene expression program, positioning this promising therapeutic target modulate pathologically stiff tissues.

Язык: Английский

Процитировано

0
Advances in Biomaterial Mechanical Properties to Regulate Macrophage Polarization DOI Open Access

恒基 贾

Journal of Clinical Personalized Medicine, Год журнала: 2025, Номер 04(02), С. 792 - 799

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
The Molecular Mechanism of Macrophages in Response to Mechanical Stress DOI

Yuntong Su,

Xing Yin

Annals of Biomedical Engineering, Год журнала: 2024, Номер 53(2), С. 318 - 330

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

2
Potential effects of biomaterials on macrophage function and their signalling pathways DOI
Fujun Zhu,

Shaolian Wang,

Xianglian Zhu

и другие.

Biomaterials Science, Год журнала: 2023, Номер 11(21), С. 6977 - 7002

Опубликована: Янв. 1, 2023

The polarization of macrophage mediated by the physicochemical properties biomaterials.

Язык: Английский

Процитировано

6
Dental-derived stem cells in tissue engineering: the role of biomaterials and host response DOI Creative Commons
Weihao Yuan, Luiza de Almeida Queiroz Ferreira, Bo Yu

и другие.

Regenerative Biomaterials, Год журнала: 2023, Номер 11

Опубликована: Ноя. 10, 2023

Abstract Dental-derived stem cells (DSCs) are attractive cell sources due to their easy access, superior growth capacity and low immunogenicity. They can respond multiple extracellular matrix signals, which provide biophysical biochemical cues regulate the fate of residing cells. However, direct transplantation DSCs suffers from poor proliferation differentiation toward functional survival rates local inflammation. Recently, elegant advances in design novel biomaterials have been made give promise use biomimetic various behaviors, including proliferation, migration. Biomaterials could be tailored with functionalities, e.g., stimuli-responsiveness. There is an emerging need summarize recent engineered biomaterials-mediated delivery therapy potential applications. Herein, we outlined for supporting host response transplantation.

Язык: Английский

Процитировано

6
Understanding Macrophage‐Tumor Interactions: Insights from Single‐Cell Behavior Monitoring in a Sessile Microdroplet System DOI
Jiaxu Lin, Qiang Zhang, Tianze Xie

и другие.

Small Methods, Год журнала: 2024, Номер unknown

Опубликована: Апрель 16, 2024

Abstract Interaction between tumor‐associated macrophages and tumor cells is crucial for development, metastasis, the related immune process. However, are highly heterogeneous spanning from anti‐tumorigenic to pro‐tumorigenic, which needs be understood at single‐cell level. Herein, a sessile microdroplet system designed monitoring cellular behavior analyzing intercellular interaction, demonstrated with macrophage‐tumor cell pairs presented. An automatic procedure based on inkjet printing method utilized precise pairing co‐encapsulation of heterotypic within picoliter droplets. The nature microdroplets ensures controlled fusion provides stable environments conducive adherent culture. nitric oxide generation morphological changes over incubation explored reveal complicated interactions perspective. response under distinct microenvironments recorded. results demonstrate that microenvironment displays modulating role in polarizing into pro‐tumorigenic phenotype. approach versatile compatible platform investigate interaction level, showing promising potential advancing studies.

Язык: Английский

Процитировано

1