Lab on a Chip, Год журнала: 2023, Номер 23(24), С. 5165 - 5172
Опубликована: Янв. 1, 2023
The utilization of microfluidic analysis technology has resulted in the advancement fast pathogenic bacteria detection, which can accurately provide information on biochemical reactions a single cell.
Язык: Английский
Small Methods, Год журнала: 2025, Номер unknown
Опубликована: Янв. 2, 2025
Abstract The rapid, precise, and automated diagnosis of infectious diseases is crucial for effective disease management control. Herein, the integrated portable automatic digital detection system (IPADS), a novel diagnostic platform nucleic acid introduced. device employs hybrid magnetic (HMS), which uses an electromagnet movable permanent magnet to modulate field control bead movement, increasing extraction efficiency over 80%, while simplifying traditional labor‐intensive process enabling quick, low‐risk sample processing. Additionally, disposable cartridge designed HMS based preprocessing, with performed using RPA‐Cas12a, enclosed, automation‐friendly across dynamic range spanning five orders magnitude, sensitivity as low 100 copies mL −1 in serum samples. An further optimizes workflow. As proof concept, IPADS applied detect hepatitis B virus (HBV) DNA 20 clinical samples, demonstrating high concordance gold‐standard quantitative PCR (qPCR) methods. These results validate potential reliable point‐of‐care testing solution.
Язык: Английский
Процитировано
1
Chinese Chemical Letters, Год журнала: 2025, Номер unknown, С. 111109 - 111109
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
1
Micromachines, Год журнала: 2024, Номер 15(3), С. 378 - 378
Опубликована: Март 12, 2024
β-cyclodextrin (β-CD) is a water-soluble, non-toxic, biocompatible, and cage compound that contains six, seven, or eight α-(1–4)-attached D-glucopyranose residues. The hydroxyl group in the β-CD responsible for reduction of metal ions as well stabilizing nanoparticles. In this study, we developed colorimetric assay identifying contagious pathogens such SARS-CoV-2 Enterococcus faecium (E. faecium) via situ development β-CD-stabilized silver nanoparticles (AgNPs). process, LAMP amplicons produced complex with nitrate (LAMP amplicon–Ag+) which was reduced when heated at 65 °C 5 min presence brown color. limit detection determined to be approximately 101 CFU mL−1 10 fg µL−1 E. SARS-CoV-2, respectively. Significantly, examination diseases completed less than 50 min, including process. Owing high sensitivity rapid readout mechanism AgNP-based assay, it anticipated introduced method can efficiently utilized versatile point-of-care testing (POCT) platform molecular diagnostics resource-limited areas.
Язык: Английский
Процитировано
4
Biosensors, Год журнала: 2024, Номер 14(8), С. 379 - 379
Опубликована: Авг. 6, 2024
In the rapid development of molecular biology, nucleic acid amplification detection technology has received more and attention. The traditional polymerase chain reaction (PCR) instrument poor refrigeration performance during its transition from a high temperature to low in cycle, resulting longer PCR cycle. Peltier element equipped with both heating cooling functions was used, while robust adaptive fuzzy proportional integral derivative (PID) algorithm also utilized as fundamental control mechanism. were switched through state machine mode, module designed achieve change. Cycle test results showed that PID used accurately rise fall (average rising speed = 11 °C/s, average falling 8 °C/s) preventing overcharging, maintaining stability, achieving ultra-fast processes (45 cycle time < 19 min). quantitative show different amounts fluorescence signals can be observed according concentrations added viral particles, an analytical limit (LoD) 10 copies per μL achieved no false positive negative control. TEC rate, sensitivity, stability. This study intended solve problem where existing thermal finds it difficult meet various new requirements, such rapid, efficient, miniaturization PCR.
Язык: Английский
Процитировано
3
TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118299 - 118299
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0
Опубликована: Янв. 10, 2025
Язык: Английский
Процитировано
0
Micromachines, Год журнала: 2024, Номер 15(12), С. 1524 - 1524
Опубликована: Дек. 21, 2024
Lab-on-a-chip (LOC) devices have been developed for nucleic acid analysis by integrating complex laboratory functions onto a miniaturized chip, enabling rapid, cost-effective, and highly sensitive on-site testing. This review examines the application of LOC technology in food safety, specifically context acid-based analyses detecting pathogens contaminants. We focus on microfluidic-based that optimize extraction purification chip or amplification detection processes based isothermal polymerase chain reaction. also explore advancements integrated combine extraction, amplification, within single to minimize sample preparation time enhance testing accuracy. The concludes with insights into future trends, particularly development portable technologies rapid efficient safety.
Язык: Английский
Процитировано
2
Critical Reviews in Analytical Chemistry, Год журнала: 2023, Номер unknown, С. 1 - 23
Опубликована: Дек. 1, 2023
AbstractSaliva is one of the most critical human body fluids that can reflect state body. The detection saliva great significance for disease diagnosis and health monitoring. Microfluidics, characterized by microscale size high integration, an ideal platform development rapid low-cost diagnostic techniques devices. Microfluidic-based testing methods have aroused considerable interest due to increasing need noninvasive frequent or long-term testing. This review briefly described analysis generally classified targets in into pathogenic microorganisms, inorganic substances, organic substances. By using this classification as a benchmark, state-of-the-art research results on microfluidic various substances were summarized. work also put forward challenges future directions saliva.Keywords: Analysismicrofluidicssaliva Disclosure statementThe authors declare they no known competing financial interests personal relationships could appeared influence reported paper.Data availability statementNo data was used article.Author contributionsX.L., XT.S. HZ.Y. conceptualized idea review. X.L. wrote initial drafts manuscript. XY.Z., XJ.L., YX.W. TL.W. contributed collection graph making. S.L. writing manuscript improvement. All reviewing gave approval final version manuscript.Additional informationFundingThis funded project from China Post-doctoral Science Foundation (No. 2021MD703911), Natural Liaoning Province 2020-MS-166), Education Department QN2019035), National 81500897).
Язык: Английский
Процитировано
1
The Journal of Physical Chemistry C, Год журнала: 2024, Номер 128(27), С. 11298 - 11305
Опубликована: Июнь 29, 2024
Narrow plasmonic resonances in an asymmetric refractive index (RI) environment under normal incidence are desired for miniaturized sensors clinical evaluation and lab-on-a-chip devices. We demonstrate that a thin alumina layer between Au nanoparticle (NP) lattices quartz substrate can induce total internal reflections nonvanishing out-of-plane electric fields, resulting ultranarrow quadrupole surface lattice (SLRs). The SLRs show line width below 1 nm with high-quality factors up to 1865 simulations 640 experiments the visible range. High-performance RI sensing based on is demonstrated glucose aqueous solutions DMSO/water mixtures, which one magnitude improvement figure of merit compared NP bare substrate. This modification opens new avenue design generate light-matter interactions applications.
Язык: Английский
Процитировано
0
Journal of Advances in Physical Chemistry, Год журнала: 2024, Номер 13(03), С. 375 - 384
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0