Опубликована: Янв. 1, 2024
Язык: Английский
Chem & Bio Engineering, Год журнала: 2024, Номер 1(11), С. 916 - 933
Опубликована: Окт. 8, 2024
The extracellular matrix (ECM) performs both as a static scaffold and dynamic, viscoelastic milieu that actively participates in cell signaling mechanical feedback loops. Recently, biomaterials with tunable properties have been utilized to mimic the native ECM fields of tissue engineering regenerative medicines. These materials can be designed support attachment, proliferation, differentiation, facilitating repair or replacement damaged tissues. Moreover, viscoelasticity modulation mimicry helps develop therapeutic strategies for diseases involving altered tissues such fibrosis cancer. study biomaterial thus intersects broad spectrum biological medical disciplines, offering insights into fundamental biology practical solutions improving human health. This review delves design fabrication hydrogels, focusing particularly on two major parameters, strength stress relaxation, how hydrogel mechanics influence interactions between living cells surrounding microenvironments. Meanwhile, this discusses current bottlenecks hydrogel-cell studies, highlighting challenges parameter decoupling, long-term stable maintenance microenvironment, general applicability testing standards conversion protocols.
Язык: Английский
Процитировано
1
Frontiers in Materials, Год журнала: 2024, Номер 11
Опубликована: Окт. 15, 2024
Regeneration of soft tissues, especially those requiring complex vascularization, is a major challenge in the field tissue engineering. The current types engineering scaffolds include sponges, electric spinning silk, hydrogels, and 3D printed biomaterials. Among them, hydrogels have unique property mimicking extracellular matrix (ECM), which can provide relatively stable microenvironment for cellular activities facilitate cell adhesion, proliferation, differentiation; thus, become promising scaffold. In this paper, we present review commonly used natural their applications as vascularization. First, enumerate importance advantages several origin terms fabricating vascularized tissues or organs. Second, discuss two different formation modalities blood vessels, well hydrogel-based vascularization strategies, including carrying growth factors, stem delivery, special scaffold structures pharmaceutical-enhanced strategy. addition, describe crosslinking strategies regeneration factors affecting it. Finally, new insights are provided development research.
Язык: Английский
Процитировано
1
PLoS ONE, Год журнала: 2023, Номер 18(9), С. e0291942 - e0291942
Опубликована: Сен. 26, 2023
Complex evolutionary dynamics governing the drug resistance is one of major challenges in cancer treatment. Understanding these mechanisms requires a sequencing technology with higher resolution to delineate whether pre-existing or de novo are behind resistance. Combining this clinically very relevant model system, namely 3D spheroids, better mimicking tumorigenesis and have so far been lacking. Thus, we sought establish dabrafenib irinotecan resistant derivatives barcoded spheroids ultimate aim quantify selection-induced clonal identify genomic determinants system. We found that induced 3D-HT-29 3D-HCT-116 mediated by barcodes, indicating presence polyclonal Moreover, whole-exome analysis chromosomal gains mutations associated spheroids. Last, show also multiple detection upregulation efflux pumps, ABCB1 ABCG2, our spheroid Overall, present quantification for first time using cellular barcoding underlying
Язык: Английский
Процитировано
3
Chemical Communications, Год журнала: 2023, Номер 60(3), С. 316 - 319
Опубликована: Ноя. 29, 2023
A physically crosslinked protein-based hydrogel was developed based on silk acid. It can be produced under physiological conditions with a modulus close to the extracellular matrix, supporting 3D cell culture and cell-laden injection.
Язык: Английский
Процитировано
2
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0