Authorea (Authorea),
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 30, 2024
As
an
important
endogenous
gasotransmitter,
hydrogen
sulfide
(H2S)
plays
a
critical
role
in
various
physiological
functions,
and
has
been
regarded
as
biomarker
of
cancer
due
to
its
overexpression
cells.
In
addition,
the
early
stages
are
often
accompanied
by
abnormalities
intracellular
microenvironments.
However,
deep
insights
into
simultaneous
detection
H2S
viscosity/polarity
variations
cells/tissues
rarely
reported.
this
work,
we
developed
near-infrared
(NIR)
mitochondria-targeting
fluorescent
probe
PDQHS,
which
exhibits
high
selectivity
for
with
emission
peak
around
632
nm,
excellent
response
through
NIR
beyond
706
nm.
Meanwhile,
PDQHS
shows
good
biocompatibility,
can
specifically
accumulate
mitochondria.
Using
visual
distinguishing
cells
from
normal
was
achieved
via
dual-channel
viscosity/polarity.
More
importantly,
successfully
applied
diagnosis
tumor
tissue.
Obviously,
monitoring
multi-markers
is
conducive
amplify
signal
compared
de-tection
single
biomarker,
thus
providing
more
sensitive
reliable
imaging
tool
diagnosis.
Abstract
Phototheranostics
has
garnered
sustained
attention
due
to
its
significant
potential
for
revolutionizing
conventional
cancer
treatment
strategies.
While
being
one
of
the
most
commonly
employed
strategies
constructing
phototheranostic
systems
by
engineering
integration
photosensitizers
(PSs)
into
nanosystems,
nano‐PSs
face
challenges
including
complexity
in
preparation
process,
low
delivery
efficiency,
and
toxicity
issues.
Contrastingly,
burgeoning
popularity
small
molecule
PSs
characterized
aggregation‐induced
emission
(AIE)
become
evident
arena
phototheranostics.
This
preference
is
underscored
their
well‐defined
structures,
adjustable
photophysical
properties,
toxicity.
Therefore,
acquiring
profound
insights
pioneering
strides
achievable
through
a
solitary
PS
with
AIE
tumor
phototheranostics
paramount
scientific
significance.
In
this
review,
we
will
discuss
recent
progress
properties
diagnosis
phototherapies
representative
examples,
guided
ethos
“Complexity
made
easy”.
We
also
look
forward
future
development
direction
molecules,
central
objective
advancing
research
focal
emphasis
on
simplicity,
expeditiousness,
safety.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Янв. 16, 2024
Abstract
Early
diagnosis
and
intervention
of
Alzheimer’s
disease
(AD)
are
particularly
important
to
delay
the
pathological
progression.
Although
fluorescent
probes
have
been
widely
employed
for
investigating
diagnosing
AD,
their
biological
applications
significantly
restricted
due
low
penetration
ability
blood–brain
barrier
(BBB)
in
vivo.
In
this
study,
we
reported
first
Golgi-targeted
two-photon
(TP)
probe,
DCM-DH,
detecting
viscosity
Golgi
apparatus.
The
probe
was
rationally
designed
exhibit
superior
analytical
performance
including
high
sensitivity,
specific
Golgi-targeting,
efficient
BBB
ability,
deep
tissue
(247
μm)
brains
AD
model
mice.
Using
demonstrated
that
fluorescence
intensity
human
liver
cancer
cell
(HepG2
cells)
higher
than
normal
(LO2
cells),
brain
mice
increased
significantly.
We
anticipate
competent
tool
could
be
easily
extended
other
biomarkers
fundamental
research
on
detrimental
disease.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 16, 2025
Cancer
is
a
global
health
challenge
that
urgently
requires
more
sensitive
and
effective
cancer
detection
methods.
Fluorescence
imaging
with
small
molecule
fluorescent
probes
has
shown
great
promise
for
but
most
of
the
developed
lack
active
tumor
cell
targeting,
which
makes
them
unable
to
selectively
target
tumors,
thereby
reducing
accuracy
in
vivo
detection.
Herein,
we
report
novel
probe
Bio-S
combines
viscosity-sensitive
membrane
targetable
group
biotin
targeted
precise
visualization
cells
tumors.
exhibits
fluorescence
changes
viscosity
at
∼660
nm
excellent
localization
ability
(red
fluorescence,
wash-free,
long-term
imaging).
Moreover,
compared
nonbiotinylated
control
C6-S,
biotinylated
can
specifically
membranes,
achieving
much
higher
selectivity
sensitivity
distinguishing
from
normal
cells.
Mice
experiments
show
tail
vein
injection
tumors
monitor
lung
metastasis
level.
Therefore,
this
work
provides
an
new
strategy
tool
tumor-targeted
diagnosis.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(14), С. 5437 - 5445
Опубликована: Март 26, 2024
The
research
on
fluorescent
rotors
for
viscosity
has
attracted
extensive
interest
to
better
comprehend
the
close
relationships
of
microviscosity
variations
with
related
diseases.
Although
scientists
have
made
great
efforts,
probes
cellular
both
aggregation-induced
emissions
(AIEs)
and
large
Stokes
shifts
improve
sensing
properties
rarely
been
reported.
Herein,
we
first
report
four
new
meso-C═N-substituted
BODIPY-based
shifts,
investigate
their
viscosity/AIE
characteristics,
perform
imaging
in
subcellular
organelles.
Interestingly,
meso-C═N-phenyl
group-substituted
probe
6
showed
an
obvious
594
nm
fluorescence
enhancement
glycerol
a
moderate
650
red
AIE
emission
water.
Further,
attaching
CF3
phenyl
group,
similar
phenomenon
was
observed
7
red-shifted
emissions,
attributed
introduction
which
plays
key
role
shifts.
Comparatively,
phenyl-group-free
probes,
meso-C═N-trifluoroethyl
group
thiazole-substituted
(8
9)
exhibited
good
viscosity-responsive
properties,
while
no
due
absence
groups.
For
experiments,
9
lysosomal
mitochondrial
targeting
respectively,
were
further
successfully
used
through
preincubation
monensin
lipopolysaccharide
(LPS),
indicating
that
C═N
polar
groups
potentially
work
as
rotatable
moieties
organelle-targeting
groups,
difference
might
be
ascribed
increased
charges
thiazole.
Therefore,
this
study,
investigated
structural
meso-C═N
respect
subcellular-targeting
ability,
viscosity,
serving
imaging.
Chemical & Biomedical Imaging,
Год журнала:
2023,
Номер
2(2), С. 126 - 134
Опубликована: Авг. 16, 2023
The
abnormal
microenvironment
parameter,
viscosity,
is
closely
connected
with
various
diffusion
processes,
signal
transduction,
molecule
interactions,
and
diseases.
It
greatly
significant
to
design
viscosity-dependent
near-infrared
(NIR)
small
fluorescence
probes
for
visualizing
biological
processes
or
diagnosing
Herein,
through
the
stepwise
modulating
structure
of
silicon–rhodamine
fluorophore
(SR),
we
report
three
viscosity
allyl
methyl
group
as
rotors,
named
SR-T-Al,
SR-S-Al,
SR-T-Me.
Among
them,
SR-T-Al
demonstrates
better
responsibility
from
1.0
1410.4
cP
viscosity.
Therefore,
probe
successfully
applied
sensitively
monitor
lysosome
microscopic
changes
living
cells
induced
by
oxygen
stress.
What's
more,
based
on
its
advantages
in
NIR
emission
(669
nm)
large
Stokes
shift
(201
nm),
also
use
it
image
variations
an
acute
hepatitis
mouse
carbon
tetrachloride.
Both
time
concentration-dependent
induction
models
display
great
ability
detect
alteration.
All
experimental
results
indicated
that
this
allyl-rotor-based
could
provide
a
general
platform
physiological
diseases
relating
