Analytical Methods,
Год журнала:
2023,
Номер
15(34), С. 4236 - 4242
Опубликована: Янв. 1, 2023
Accurate
analysis
of
miRNA
is
valuable
for
the
diagnosis
various
diseases.
Herein,
a
sensitive
and
accurate
fluorescence
method
was
developed
detection
based
on
catalytic
hairpin
assembly
(CHA)
split-G-quadruplex
(split-G4)
signal
reactions.
The
presence
target
activated
CHA
process
through
unfolding
H1
probe,
which
could
continuously
induce
proximity
split-G4.
formed
intact
G4
can
be
specifically
recognized
by
commercial
fluorescent
dye
ThT
(thioflavin
T),
allowing
highly
sensitive,
label-free
miRNAs.
By
utilizing
split-G4
to
generate
signal,
exhibited
low
background
high
reliability.
In
addition,
demonstrated
applied
clinical
sample
detection,
implying
its
promising
prospect
disease
diagnosis.
Chemical Communications,
Год журнала:
2024,
Номер
60(86), С. 12569 - 12572
Опубликована: Янв. 1, 2024
A
programmable
CRISPR-Cas12a
system
for
selective
detection
of
various
DNA
glycosylases
is
described.
By
temporarily
inactivating
Cas12a
through
the
introduction
specific
modifications
in
complementary
strand
Cas12a's
crRNA,
able
to
detect
target
glycosylases.
This
approach
addresses
critical
gaps
current
diagnostics
non-nucleic
acid
beyond
limitations
aptamers.
The Analyst,
Год журнала:
2023,
Номер
149(3), С. 729 - 734
Опубликована: Дек. 4, 2023
Nowadays,
easy,
convenient,
and
sensitive
sensing
strategies
are
still
critical
for
organophosphorus
pesticides
in
environmental
water
samples.
Herein,
a
novel
pesticide
(OP)
assay
based
on
acetylcholinesterase
(AChE)
MnO
Journal of Analytical Science & Technology,
Год журнала:
2024,
Номер
15(1)
Опубликована: Март 21, 2024
Abstract
MicroRNAs
(miRNAs),
a
category
of
small
molecules
that
possess
significant
regulatory
capabilities,
have
been
extensively
employed
as
biomarkers
in
the
domain
biosensing
to
facilitate
early
detection
diverse
ailments.
However,
sensitive
and
accurate
miRNA
remains
huge
challenge
due
high
similarity
between
homologous
sequences
low
abundance.
Therefore,
it
is
essential
develop
methods
with
sensitivity
specificity
for
detection.
In
this
study,
we
present
development
signal
cycle-based
platform
utilizes
cyclic
reverse
transcription
(CRT)
CRISPR-Cas12a
enable
precise
microRNAs.
The
CRT
mechanism
facilitates
target
recognition
presence
miRNA,
thereby
converting
signals
DNA
signals.
trans
-cleavage
activity
Cas12a
protein
triggered
by
formation
complete
hairpin-shaped
products;
results
cleavage
section
contained
H
probe,
while
RNA
(“4”@MBs)
loaded
onto
surface
magnetic
beads
(MB).
By
binding
“reporter”
sensor,
“4”
create
an
RNA/DNA
duplex
duplex-specific
nuclease
(DSN)
can
recognize.
probe
thus
metabolized,
leading
reappearance
fluorescence
signal.
capitalizing
on
exceptional
fidelity
selectivity
CRISPR/Cas12a,
well
substantial
impact
enzymatic
cycle
amplification,
approach
demonstrated
remarkable
detection,
even
complex
environment
containing
10%
fetal
bovine
serum
(FBS)
sample.
contrast,
limit
3.2
fM
conceivable.
Furthermore,
maintained
notable
degree
stability,
which
was
anticipated
result
miRNAs
effective
manner.
