Dalton Transactions, Год журнала: 2024, Номер unknown
Опубликована: Дек. 28, 2024
Schematic diagram of the Ce-doped MOF nanozymes that mimic peroxidase enzyme. The corresponding mechanistic cycle is also shown.
Язык: Английский
Journal of the American Chemical Society, Год журнала: 2024, Номер 146(40), С. 27486 - 27498
Опубликована: Авг. 28, 2024
The improvement of hydrogen evolution reaction kinetics can be largely accelerated by introducing a well-designed spillover pathway into the catalysts. However, driving force and mechanism migration on surface catalysts are poorly understood rarely explored in depth. Here, inspired specific ferroelectric property HfO
Язык: Английский
Процитировано
15
Chemical Engineering Journal, Год журнала: 2024, Номер 486, С. 150268 - 150268
Опубликована: Март 8, 2024
Язык: Английский
Процитировано
14
Advanced Functional Materials, Год журнала: 2025, Номер unknown
Опубликована: Апрель 3, 2025
Abstract Non‐thermal bioelectricity cancer therapy (BECT), utilizing high‐voltage and high‐frequency pulsed electric fields, represents a novel approach in oncological treatment. Herein, an pulse‐regulated MXene‐based nanozyme (MXenzyme)‐catalyzed cell eradication is presented while activating immunogenic responses for integrative BECT immunotherapy. Ti₃C₂ MXenzyme, the most conductive member of MXene family, synthesized characterized superior electrical properties aqueous environment. Incorporating MXenzyme into significantly enhances irreversible death expands ablation area compared to alone. Computational modeling reveals that on membranes generates localized hotspots current density field (E‐field) concentration during application. catalytic effect induced three primary cytotoxic mechanisms: 1) direct membrane depolarization poration, 2) disruption voltage‐gated ion channels, 3) generation reactive oxygen species. These combined mechanisms contributed death. Immunological profiling tumor microenvironment modulated by MXenzyme‐BECT confirmed its profound impact activation potent anti‐tumor immune response. Translational potential evaluated using computational modeling‐based pre‐treatment planning combination with checkpoint inhibitor therapy. Findings underscore MXenzyme's pivotal role as highly electric‐regulated enzymatic catalyst, enhancing efficacy advancing BECT‐immunotherapy strategies.
Язык: Английский
Процитировано
1
Biosensors and Bioelectronics, Год журнала: 2024, Номер 261, С. 116468 - 116468
Опубликована: Июнь 5, 2024
Язык: Английский
Процитировано
8
Organic & Biomolecular Chemistry, Год журнала: 2024, Номер 22(18), С. 3559 - 3583
Опубликована: Янв. 1, 2024
This review highlights the recent advancements in enzymatic modifications of steroid scaffolds, emphasizing hydroxylation, ketoreduction, dehydrogenation, cascade reactions, and other modifications.
Язык: Английский
Процитировано
6
Biomedicines, Год журнала: 2024, Номер 12(1), С. 152 - 152
Опубликована: Янв. 11, 2024
We describe a bielectrode system for evaluation of the electrocatalytic activity cytochrome P450 2E1 (CYP2E1) towards chlorzoxazone. One electrode was employed to immobilize Bactosomes with human CYP2E1, reductase (CPR), and b5 (cyt b5). The second used quantify CYP2E1-produced 6-hydroxychlorzoxazone by its direct electrochemical oxidation, registered using square-wave voltammetry. Using this system, we determined steady-state kinetic parameters chlorzoxazone hydroxylation CYP2E1 immobilized on electrode: maximal reaction rate (Vmax) 1.64 ± 0.08 min−1, Michaelis constant (KM) 78 9 μM. studied characteristics have revealed that electron transfer from occurs both flavin prosthetic groups CPR heme iron ions cyt b5. Additionally, it has been demonstrated capacity activate chlorzoxazone, likely through intermolecular electrochemically reduced form ion.
Язык: Английский
Процитировано
5
Polymer, Год журнала: 2024, Номер 307, С. 127230 - 127230
Опубликована: Июнь 3, 2024
Язык: Английский
Процитировано
5
Bioelectrochemistry, Год журнала: 2025, Номер 163, С. 108913 - 108913
Опубликована: Янв. 22, 2025
The ability of the living world to flourish in face constant exposure dangerous chemicals depends on management a widespread group enzymes known as heme-thiolate monooxygenases or cytochrome P450 superfamily. About three-quarters all reactions determining metabolism endogenous compounds, those carried foods, taken drugs, even synthetic discarded into environment depend their catalytic performance. chromatographic and (photo)luminometric methods routinely used predictive analytical tools laboratories have significant drawbacks ranging from limited shelf-life reagents, use substrates, laborious tedious procedures for highly sensitive detection. In this review, alternative electrochemical biosensors using bio-element are emphasized main aspects well regarding implementation usefulness. Despite various schemes proposed implementation, reports real applications scant several reasons, including low reaction rates, broad substrate specificity, uncoupling occurrence, need expensive electron transfer partners promote transfer. Finally, prospect future developments is introduced, focusing integrating miniaturized systems with techniques, alongside optimizing enzyme immobilization electrode modifications improve enzymatic stability enhance sensor reliability. This progress represents crucial step towards creation portable that mimic human physiological responses, supporting precision medicine approach.
Язык: Английский
Процитировано
0
Metabolites, Год журнала: 2025, Номер 15(3), С. 151 - 151
Опубликована: Фев. 23, 2025
Background: Heme is an important cofactor and plays crucial roles in the correct folding of hemoproteins. The synthesis heme can be enhanced by plasmid-based expression biosynthetic genes. However, genetically unstable requires utilization antibiotics to maintain high copy numbers plasmids. Methods: rate-limiting steps biosynthesis were first analyzed based on previous studies accumulation intermediates was achieved adding precursor (5-aminolevulinic acid, ALA). Next, intracellular porphyrin increased deleting transporter TolC. Finally, synthetic genes modified integrating hemA hemL into cheW yciQ locus, assembling enzymes HemC HemD with RIAD-RIDD tags, replacing promoters hemE/hemH constitutive promoter PJ23100, degradation gene yfeX. Results: An supply HEME2 strain obtained a titer 0.14 mg/L, which 4.60-fold higher than that C41(DE3) strain. applied produce human hemoglobin leghemoglobin. peroxidase activity 1.29-fold 42.4% HEME2-hHb values control C41-hHb. In addition, content leghemoglobin 39.2% 53.4% HEME2-sHb compared C41-sHb. Conclusions: A plasmid-free Escherichia coli capable efficient stable constructed used for production high-active hemoglobins.
Язык: Английский
Процитировано
0
Journal of Flow Chemistry, Год журнала: 2023, Номер 14(1), С. 357 - 366
Опубликована: Ноя. 28, 2023
Abstract We are currently experiencing a resurgence in the realm of electrochemical organic synthesis, driven by transformative potential conducting redox chemistry under mild conditions through simple use electrons, thereby circumventing harmful reductants and oxidants. This renaissance is further bolstered fusion electrochemistry with flow chemistry, which not only grants precise control over reaction parameters but also promotes sustainability heightened reproducibility. Despite these promising advancements, application to steer asymmetric processes remains its nascent stage. perspective delves into limited contributions date, shedding light on critical challenges presenting prospective solutions that essential for fully unleashing untapped this field. Graphical abstract
Язык: Английский
Процитировано
4