Red/near-infrared light triggered photorelease via sensitized photolysis

Coordination Chemistry Reviews, Год журнала: 2024, Номер 518, С. 216117 - 216117

Опубликована: Июль 27, 2024

Язык: Английский

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Combined Mutational and Spectroscopic Study on the Calcium-Related Kinetic Effects on the VirChR1 Photocycle

The Journal of Physical Chemistry B, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

The viral rhodopsin 1 subfamily consists of microbial rhodopsins, such as VirChR1, with a light-gated cation channeling functionality, which is inhibited by calcium. For S14, E54, and N225 have been proposed key residues for calcium binding. They form highly conserved SEN-triad in channelrhodopsins near the functionally important central gate. Here, we present time-resolved UV/vis spectroscopic study on VirChR1 variants S14A, E54A, N225A calcium-dependent manner. Comparison calcium-associated effects observed wild type shed light role respective interaction. While S14A shows less pronounced, yet similar, signals, indicative reduced affinity, E54A exhibits nearly calcium-independent photocycle kinetics, highlighting its crucial variant altered both absence presence calcium, demonstrating critical formation gate VirChR1.

Язык: Английский

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Dual-key cooperatively activated DNA regulator for controlling mitochondria-lysosome interactions

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 13, 2025

Mitochondria-lysosome interactions are critical for maintaining cellular homeostasis. Although genetically encoded protein based optogenetic technique has been developed to regulate such interactions, it still suffers from shortcomings including complicated operation and potential interference organelle functions. Here, we present a fast, simple, biocompatible programmable platform via activable DNA regulators achieve spatiotemporal regulation of mitochondria-lysosome in living cells. In our system, two locked regulators, OK-MLIR DK-MLIR, that could be respectively activated with UV light (One Key) as well endogenous glutathione (Dual Keys), were modularly designed modulating contacts. We have shown these can used facilitating mitochondrial fission autophagy. Moreover, the DK-MLIR enables selective efficient manipulation target cell migration proliferation highly temporal spatial controllability. This modular design principle provides new interaction study, precision therapy.

Язык: Английский

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Elucidation of Expression Patterns and Functional Properties of Archaerhodopsin Derived from Halorubrum sp. Ejinoor

Biology, Год журнала: 2025, Номер 14(4), С. 360 - 360

Опубликована: Март 31, 2025

This study elucidates the structural determinants and optogenetic potential of Archaerhodopsin HeAR, a proton pump from Halorubrum sp. Ejinoor isolated Inner Mongolian salt lakes. Through heterologous expression in E. coli BL21 (DE3) integrative biophysical analyses, we demonstrate that HeAR adopts stable trimeric architecture (129 kDa) with detergent-binding characteristics mirroring bacteriorhodopsin (BR); however, it exhibits 10 nm bathochromic spectral shift (λmax = 550 nm) elevated affinity (Asp-95 pKa 3.5 vs. BR Asp-85 2.6), indicative evolutionary optimization its retinal-binding electrostatic microenvironment. Kinetic profiling reveals HeAR’s prolonged photocycle (100 ms BR’s 11 ms), marked by rapid M-state decay (3.3 ms) extended dark-adaptation half-life (160 min), bistable behavior attributed to enhanced hydrogen bond persistence (80%) reduced conformational entropy (RMSD 2.0 Å). Functional assays confirm light-driven extrusion (0.1 ng H⁺/mg·s) DCCD-amplified flux (0.3 ATP synthesis nmol/mg·s), underscoring synergy H⁺-ATPase. Phylogenetic analyses reveal 95% homology AR4 conservation proton-wire residues, despite divergent Trp/Tyr/Ser networks redefine chromophore stabilization. AlphaFold-predicted models (TM-score > 0.92) molecular docking identify superior retinoid-binding (ΔG −12.27 kcal/mol), while specificity (550–560 acid-stable photoresponse highlight adaptability for low-irradiance neuromodulation. These findings position as precision tool, circumventing overlap excitatory opsins enabling sustained hyperpolarization minimized phototoxicity. By bridging microbial energetics optobioengineering, this work expands archaeal rhodopsin toolkit provides blueprint designing wavelength-optimized photoregulatory systems.

Язык: Английский

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Production of eukaryotic heliorhodopsins for structural analysis utilizing the LEXSY expression system

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 283, С. 137324 - 137324

Опубликована: Ноя. 6, 2024

Язык: Английский

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